ISSR and RAPD based evaluation of genetic fidelity and active ingredient analysis of regenerated plants of Picrorhiza kurroa

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• 作者：Janhvi Mishra Rawat (1) (2)
  Balwant Rawat (2) (3)
  Shakti Mehrotra (4)
  Anup Chandra (1)
  Subhash Nautiyal (1)
  
• 关键词：Genetic fidelity ; ISSR ; RAPD ; Plant tissue culture ; West Himalaya
• 刊名：Acta Physiologiae Plantarum
• 出版年：2013
• 出版时间：June 2013
• 年：2013
• 卷：35
• 期：6
• 页码：1797-1805
• 全文大小：441KB
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• 作者单位：Janhvi Mishra Rawat (1) (2)
  Balwant Rawat (2) (3)
  Shakti Mehrotra (4)
  Anup Chandra (1)
  Subhash Nautiyal (1)
  
  1. Botany Division, Forest Research Institute, Dehradun, 248006, India
  2. GB Pant Institute of Himalayan Environment and Development, Kosi-Katarmal, Almora, 263 643, Uttarakhand, India
  3. Society for the Conservation of Nature, Anantapur University Road, Rewa, 486 002, Madhya Pradesh, India
  4. Plant Biotechnology Division, Central Institute of Medicinal and Aromatic Plants, Lucknow, 226 015, India
  

文摘

Genetic stability and phytochemical analysis of in vitro established plants of Picrorhiza kurroa Royle ex Benth, have been carried out. Random amplified polymorphic DNA (RAPD) and inter simple sequence repeats (ISSR) markers were used to assess the genetic fidelity of tissue culture products including three adventitious shoots from three calli and 6?months old tissue culture raised plants growing in green house condition with mother plant. Apparent genetic variation was detected in the five types of plant materials. The percentage of polymorphic bands in the RAPD and ISSR analysis were 16.25 and 14.54?%, respectively. The genetic similarity was calculated on the basis of RAPD and ISSR data among the five types of plant materials and were ranged from 0.5 to 1.0 (mean 0.75) and 0.47 to 1.0 (mean 0.73), respectively. The similarity coefficient by both RAPD and ISSR analysis revealed that differences between tissue culture raised plants and mother plant was not remarkable, but notable differences were observed among three adventitious shoots regenerated from three calli. The phytochemical analysis of tissue culture raised products showed higher secondary metabolite (picrotin and picrotoxinin) content as compare to mother plant. The information gained on genetic stability/variability will be valuable for the large scale propagation and secondary metabolite production of P. kurroa.