Development of 31 polymorphic microsatellite markers for the mole salamander (Ambystoma talpoideum) using Illumina paired-end sequencing

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• 作者：Cara N. Love (1)
  R. Wesley Flynn (1)
  Schyler O. Nunziata (1)
  Kenneth L. Jones (2)
  Stacey L. Lance (1)
  
• 关键词：Ambystoma ; Illumina ; Microsatellite ; PAL_FINDER ; PCR primers ; SSR
• 刊名：Conservation Genetics Resources
• 出版年：2013
• 出版时间：December 2013
• 年：2013
• 卷：5
• 期：4
• 页码：951-954
• 全文大小：
• 作者单位：Cara N. Love (1)
  R. Wesley Flynn (1)
  Schyler O. Nunziata (1)
  Kenneth L. Jones (2)
  Stacey L. Lance (1)
  
  1. Savannah River Ecology Laboratory, University of Georgia, Drawer E, Aiken, SC, 29802, USA
  2. Department of Biochemistry and Molecular Genetics, University of Colorado School of Medicine, Aurora, CO, 80045, USA
  
• ISSN：1877-7260

文摘

We isolated and characterized a total of 31 microsatellite loci from the mole salamander, Ambystoma talpoideum. Loci were screened in 20 individuals from a single location in Aiken, South Carolina. The number of alleles per locus ranged from 3 to 11, observed heterozygosity ranged from 0.000 to 0.700, and the probability of identity values ranged from 0.031 to 0.400. These new loci will provide tools for examining the genetic diversity, structure, mating system, and adult morph determination of A. talpoideum.