PET Imaging of Stroke-Induced Neuroinflammation in Mice Using [18F]PBR06

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• 作者：Frederick M. Lartey (1)
  G-One Ahn (1) (6)
  Bin Shen (3)
  Keith-Travis Cord (1)
  Tenille Smith (4) (9)
  Joshua Y. Chua (4)
  Sahar Rosenblum (4) (9)
  Hongguang Liu (3)
  Michelle L. James (3)
  Sophia Chernikova (1)
  Star W. Lee (1) (4) (8)
  Laura J. Pisani (3)
  Rabindra Tirouvanziam (10) (5)
  John W. Chen (7)
  Theo D. Palmer (11)
  Frederick T. Chin (3)
  Raphael Guzman (4) (9)
  Edward E. Graves (1) (2)
  Billy W. Loo Jr. (1) (2)
  
• 关键词：Stroke ; Neuroinflammation ; Translocator protein 18?kDa (TSPO) ; Peripheral benzodiazepine receptor (PBR) ; Positron emission tomography (PET) ; [18F]PBR06
• 刊名：Molecular Imaging and Biology
• 出版年：2014
• 出版时间：February 2014
• 年：2014
• 卷：16
• 期：1
• 页码：109-117
• 全文大小：515 KB
• 作者单位：Frederick M. Lartey (1)
  G-One Ahn (1) (6)
  Bin Shen (3)
  Keith-Travis Cord (1)
  Tenille Smith (4) (9)
  Joshua Y. Chua (4)
  Sahar Rosenblum (4) (9)
  Hongguang Liu (3)
  Michelle L. James (3)
  Sophia Chernikova (1)
  Star W. Lee (1) (4) (8)
  Laura J. Pisani (3)
  Rabindra Tirouvanziam (10) (5)
  John W. Chen (7)
  Theo D. Palmer (11)
  Frederick T. Chin (3)
  Raphael Guzman (4) (9)
  Edward E. Graves (1) (2)
  Billy W. Loo Jr. (1) (2)
  
  1. Department of Radiation Oncology, Stanford University School of Medicine, Stanford, CA, USA
  6. Integrative Biosciences & Biotechnology, Pohang University of Science & Technology, Kyungbuk, 790-784, Korea
  3. Department of Radiology, Stanford University School of Medicine, Stanford, CA, USA
  4. Department of Neurosurgery, Stanford University School of Medicine, Stanford, CA, USA
  9. Departments of Neurosurgery and Biomedicine, University of Basel, Basel, Switzerland
  8. Laboratory of Genetics, The Salk Institute for Biological Studies, La Jolla, CA, USA
  10. Department of Pediatrics, Emory-?Children’s Center for CF Research, Emory University School of Medicine, Atlanta, GA, USA
  5. Department of Pediatrics, Stanford University School of Medicine, Stanford, CA, USA
  7. Center for Systems Biology and Department of Radiology, Massachusetts General Hospital, Harvard Medical School, Boston, MA, USA
  11. Institute for Stem Cell Biology and Regenerative Medicine, Stanford University School of Medicine, Stanford, CA, USA
  2. Stanford Cancer Institute, Stanford, CA, USA
  
• ISSN：1860-2002

文摘

Purpose The purpose of this study is to evaluate the 18?kDa translocator protein (TSPO) radioligand [18F]N-fluoroacetyl-N-(2,5-dimethoxybenzyl)-2-phenoxyaniline ([18F]PBR06) as a positron emission tomography (PET) imaging biomarker of stroke-induced neuroinflammation in a rodent model. Procedures Stroke was induced by transient middle cerebral artery occlusion in Balb/c mice. Dynamic PET/CT imaging with displacement and preblocking using PK111195 was performed 3?days later. PET data were correlated with immunohistochemistry (IHC) for the activated microglial markers TSPO and CD68 and with autoradiography. Results [18F]PBR06 accumulation peaked within the first 5?min postinjection, then decreased gradually, remaining significantly higher in infarct compared to noninfarct regions. Displacement or preblocking with PK11195 eliminated the difference in [18F]PBR06 uptake between infarct and noninfarct regions. Autoradiography and IHC correlated well spatially with uptake on PET. Conclusions [18F]PBR06 PET specifically images TSPO in microglial neuroinflammation in a mouse model of stroke and shows promise for imaging and monitoring microglial activation/neuroinflammation in other disease models.