The antiproliferative effect of C2-ceramide on lung cancer cells through apoptosis by inhibiting Akt and NFκB

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• 作者：I-Ling Lin (1)
  Han-Lin Chou (2) (6)
  Jin-Ching Lee (2)
  Feng-Wei Chen (2)
  Yao Fong (3)
  Wei-Chiao Chang (4)
  Hurng Wern Huang (5)
  Chang-Yi Wu (6)
  Wen-Tsan Chang (7)
  Hui-Min David Wang (8)
  Chien-Chih Chiu (1) (2)
  
• 关键词：Lung cancer ; Apoptosis ; NSCLC ; Ceramides ; p ; Akt ; p ; NFκB ; survivin ; cyclin A2
• 刊名：Cancer Cell International
• 出版年：2014
• 出版时间：December 2014
• 年：2014
• 卷：14
• 期：1
• 全文大小：500 KB
• 作者单位：I-Ling Lin (1)
  Han-Lin Chou (2) (6)
  Jin-Ching Lee (2)
  Feng-Wei Chen (2)
  Yao Fong (3)
  Wei-Chiao Chang (4)
  Hurng Wern Huang (5)
  Chang-Yi Wu (6)
  Wen-Tsan Chang (7)
  Hui-Min David Wang (8)
  Chien-Chih Chiu (1) (2)
  
  1. Department of Medical Laboratory Science and Biotechnology, Kaohsiung Medical University, Kaohsiung 807, Taiwan
  2. Department of Biotechnology, Kaohsiung Medical University, Kaohsiung 807, Taiwan
  6. Department of Biological Sciences, National Sun Yat-Sen University, 70 Lien Hai Road, Kaohsiung 804, Taiwan
  3. Chest Surgery, Chi-Mei Foundation Medical Center, Yung Kang City, Tainan, 901, Taiwan
  4. Department of Clinical Pharmacy; Master Program for Clinical Pharmacogenomics and Pharmacoproteomics, School of Pharmacy, Taipei Medical University, Taipei, Taiwan
  5. Institute of Biomedical Sciences, National Sun Yat-Sen University, Kaohsiung 804, Taiwan
  7. Division of Hepatobiliary and Pancreatic Surgery, Department of Surgery, Kaohsiung Medical University, Kaohsiung, Taiwan
  8. Department of Fragrance and Cosmetic Science; Graduate Institute of Natural Products, Kaohsiung Medical University, Kaohsiung 807, Taiwan
  
• ISSN：1475-2867

文摘

The anticancer effects of ceramide have been reported in many types of cancers but less in lung cancer. In this study, we used C2-ceramide to further investigate its possible anticancer effects and mechanisms on non-small cell lung cancer (NSCLC) H1299 cells. The result of cell proliferation in terms of trypan blue assay showed high dose of C2-ceramide inhibited cell survival after 24 h treatment. The flow cytometry-based assays indicated the effect of apoptosis, chromatin condensation, and G1 arrest in terms of Annexin V/propidium iodide (PI), DAPI, and PI stainings, respectively. Moreover, the decreased protein level of p-Akt, p-NFκB, survivin and cyclin A2 were detected by Western blot assay. Taken together, these results indicated the antiproliferative effect of C2-ceramide is majorly responsible for cell apoptosis in lung cancer H1299 cells.