Proteomic and molecular investigations revealed that Acidithiobacillus caldus adopts multiple strategies for adaptation to NaCl stress

详细信息    查看全文

• 作者：Xu Guo (1) (3)
  Chengying Jiang (1) (2)
  Yuanming Luo (1)
  Mingjiang Zhang (1) (3)
  Ansgar Poetsch (4)
  Shuangjiang Liu (1) (2)
  
• 关键词：Acidithiobacillus caldus SM ; 1 ; NaCl stress ; Proline ; Single ; stranded DNA ; binding proteins ; Bioleaching
• 刊名：Chinese Science Bulletin
• 出版年：2014
• 出版时间：January 2014
• 年：2014
• 卷：59
• 期：3
• 页码：301-309
• 全文大小：573 KB
• 作者单位：Xu Guo (1) (3)
  Chengying Jiang (1) (2)
  Yuanming Luo (1)
  Mingjiang Zhang (1) (3)
  Ansgar Poetsch (4)
  Shuangjiang Liu (1) (2)
  
  1. State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, China
  3. University of Chinese Academy of Sciences, Beijing, 100049, China
  2. Environmental Microbiology and Biotechnology Research Center, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, China
  4. Lehrstuhl for Biochemie der Pflanzen, Ruhr Universitaet, 44801, Bochum, Germany
  
• ISSN：1861-9541

文摘

Acidithiobacillus caldus plays an important role in commercial bioleaching. To understand how NaCl stress adaptation occurs in A. caldus, we grew A. caldus strain SM-1 in media containing high NaCl concentrations. SM-1 grew at concentrations of up to 1.0-mol?L? NaCl, but growth was severely inhibited at higher concentrations. Proteomic analysis showed that SM-1 used multiple strategies to respond to NaCl stress. In addition to several heat-shock proteins, enzymes involved in proline biosynthesis increased under NaCl stress. In addition, two DNA-binding proteins and a third protein of unknown function (Atc_1291), which was subsequently identified as a putative single-stranded DNA-binding protein, were up-regulated in the presence of NaCl stress. These DNA-binding proteins might play a role in response to osmotic stress. Atc_1291 was cloned and expressed in Escherichia coli. Surprisingly, we found that E. coli BL21/pET28a-atc_1291 grew to higher cell densities than E. coli BL21/pET28a, regardless of NaCl stress. Homologs to Atc_1291 were identified in several groups of Proteobacteria. The role of Atc_1291 in enhancing cell growth needs further investigation.