Overexpression of PtSOS2 Enhances Salt Tolerance in Transgenic Poplars

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• 作者：Jie Zhou (1)
  Jingjing Wang (1)
  Yufang Bi (1)
  Like Wang (1)
  Luozhong Tang (1)
  Xiang Yu (2)
  Misato Ohtani (2)
  Taku Demura (2)
  Qiang Zhuge (1)
  
• 关键词：Populus ; PtSOS2 ; Salt tolerance ; SnRK ; Overexpression
• 刊名：Plant Molecular Biology Reporter
• 出版年：2014
• 出版时间：February 2014
• 年：2014
• 卷：32
• 期：1
• 页码：185-197
• 全文大小：1,151 KB
• 作者单位：Jie Zhou (1)
  Jingjing Wang (1)
  Yufang Bi (1)
  Like Wang (1)
  Luozhong Tang (1)
  Xiang Yu (2)
  Misato Ohtani (2)
  Taku Demura (2)
  Qiang Zhuge (1)
  
  1. Key Laboratory of Forest Genetics & Biotechnology, Ministry of Education, Nanjing Forestry University, Nanjing, 210037, China
  2. RIKEN Plant Science Center, 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama, 230-0045, Japan
  
• ISSN：1572-9818

文摘

Protein kinases are major signal transduction factors that have a central role in mediating acclimation to environmental changes in eukaryotic organisms. In this study, we cloned and identified three salt overly sensitive 2 (SOS2) genes in the woody plant Populus trichocarpa, designated as PtSOS2.1, PtSOS2.2, and PtSOS2.3, which were transformed into hybrid poplar clone T89 (Populus tremula×?Populus tremuloides Michx clone T89) mediated by Agrobacterium tumefaciens. Southern and northern blot analyses verified that the three genes integrated into the plant genome, and were expressed at a stable transcription level. Meanwhile, overexpression of all three PtSOS2 genes did not retard the growth of plants under normal conditions. Instead, it promoted growth in both agar-medium and soil conditions in response to salinity stress. Under salt stress, overexpression of PtSOS2.1, PtSOS2.2, and PtSOS2.3 increased the concentrations of proline and photosynthetic pigments, and the relative water content (RWC), and the activity of antioxidant enzymes, and decreased the malondialdehyde (MDA) concentrations in transgenic lines compared to the control. These results suggest that overexpression of PtSOS2 plays a significant role in improving the salt tolerance of poplars, reducing the damage to membrane structures, and enhancing osmotic adjustment and antioxidative enzyme regulation under salt stress.