Aptasensor for adenosine triphosphate based on electrode–supported lipid bilayer membrane

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• 作者：Peiyi Gao (1)
  Yunfeng Xia (1)
  Lele Yang (1)
  Tengfei Ma (1)
  Ling Yang (1)
  Qianqiong Guo (1)
  Shasheng Huang (1)
  
• 关键词：Electrochemical sensor ; Aptamer ; Lipid bilayer membrane ; Adenosine triphosphate
• 刊名：Microchimica Acta
• 出版年：2014
• 出版时间：January 2014
• 年：2014
• 卷：181
• 期：1-2
• 页码：205-212
• 全文大小：660 KB
• 作者单位：Peiyi Gao (1)
  Yunfeng Xia (1)
  Lele Yang (1)
  Tengfei Ma (1)
  Ling Yang (1)
  Qianqiong Guo (1)
  Shasheng Huang (1)
  
  1. Life and Environmental Science College, Shanghai Normal University, Shanghai, 200234, People’s Republic of China
  
• ISSN：1436-5073

文摘

We have developed an aptasensor for adenosine triphosphate (ATP) based on an electrode-supported lipid bilayer membrane. The assay is based on a conformational change that is induced after binding the target which modulates the electron transfer rate in the conductive path. The method is highly sensitive, stable, and repeatable. The detection limit for ATP is 50?nM, and the dynamic range extends to 3.2?μM, which covers the concentration range of ATP in cell lysates (from 0.1 to 1?μM). The method also holds promise in that it may be transferred to submicro- or nano-scale electrodes so to enable intracellular monitoring of ATP. Figure An aptasensor for adenosine triphosphate based on an electrode-supported lipid bilayer membrane in principle of target-binding induced conformational change to modulate the electron transfer rate in the conductive path.