Potato bacterial wilt in India caused by strains of phylotype I, II and IV of Ralstonia solanacearum

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• 作者：V. Sagar (1)
  A. Jeevalatha (1)
  Sarita Mian (2)
  S. K. Chakrabarti (3)
  M. S. Gurjar (4)
  R. K. Arora (5)
  S. Sharma (1)
  R. R. Bakade (6)
  B. P. Singh (1)
  
• 关键词：Bacterial wilt ; Biovar ; Diversity ; Phylotype ; Potato ; Ralstonia solanacearum ; Sequevar
• 刊名：European Journal of Plant Pathology
• 出版年：2014
• 出版时间：January 2014
• 年：2014
• 卷：138
• 期：1
• 页码：51-65
• 全文大小：534 KB
• 作者单位：V. Sagar (1)
  A. Jeevalatha (1)
  Sarita Mian (2)
  S. K. Chakrabarti (3)
  M. S. Gurjar (4)
  R. K. Arora (5)
  S. Sharma (1)
  R. R. Bakade (6)
  B. P. Singh (1)
  
  1. Central Potato Research Institute (ICAR), Shimla, 171 001, Himachal Pradesh, India
  2. Shri Guru Ram Ria Institute of Technology & Science, Dehradun, 248 001, Uttrakhand, India
  3. Central Tuber Crops Research Institute, Thiruvananthapuram, 695 017, Kerala, India
  4. Central Potato Research Station, Shillong, 793 009, Meghalaya, India
  5. Central Potato Research Station, Jalandhar, 144 003, Punjab, India
  6. Central Potato Research Station, Patna, 801 506, Bihar, India
  
• ISSN：1573-8469

文摘

Bacterial wilt or brown rot is one of the most devastating diseases of potato caused by a bacterium Ralstonia?solanacearum (Smith 1986) Yabuuchi et al. (Microbiol Immunol 39:897-04 1995). Traditionally, R. solanacearum is classified into five races (r) on the basis of differences in host range and six biovars (bvs) on the basis of biochemical properties. Recently using molecular methods, R.?solanacearum has been classified into phylotypes based on the intergenic transcribed sequence of the ribosomal RNA genes 16S and 23S and into sequevars based on the endoglucanase gene (egl) sequence. In the present study, 75 bacterial strains, isolated from wilt infected potatoes from various potato growing regions of India, were classified by traditional and molecular methods. The identity of all the strains was confirmed as R. solanacearum as expected single 280-bp fragment resulted in all the strains following PCR amplification using R. solanacearum specific universal primer pair 759/760. Biovar (bv) analysis, based on utilization of disaccharide sugars and hexose alcohols, categorised the 75 strains into bv2 (78.7?%), 2?T (5.3?%), 3 (5.3?%) and 4 (10.7?%). The phylotype specific multiplex PCR assigned 78.7?% strains to phylotype II, 16.0?% to phylotype I and 5.3?% to phylotype IV. Phylogenetic analysis of egl gene sequences clustered all fifty nine phylotype II (bv2) strains with reference strain IPO1609 (IIB-1), all four phylotype IV (bv2T) strains with reference strain MAFF301558 (IV-8), three phylotype I (bv3) strains with reference strain MAFF211479 (I-30) and all eight phylotype I (bv4) and one phylotype I (bv3) strain with reference strain CIP365 (I-45). The study concluded that the Indian potato strains of R. solanacearum belong to three out of four phylotypes namely: the Asian phylotype I, the American phylotype II, and the Indonesian phylotype IV. This is the first study to address the diversity of R. solanacearum from potato in India using phylotype and sequevar scheme. We also report here for the first time the occurrence of phylotype IV sequevar 8 (bv2T) strain of R. solanacearum causing potato bacterial wilt in mid hills of Meghalaya in India.