Association of functional polymorphisms in the MxA gene with susceptibility to enterovirus 71 infection

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• 作者：Xiaoai Zhang (1)
  Hongmei Xu (2)
  Xiaodan Chen (1) (3)
  Xiujun Li (1) (4)
  Xianjun Wang (5)
  Shujun Ding (5)
  Renli Zhang (6)
  Lijuan Liu (1)
  Cui He (1)
  Lu Zhuang (1)
  Hao Li (1)
  Panhe Zhang (1)
  Hong Yang (1)
  Tingyu Li (2)
  Wei Liu (1)
  Wuchun Cao (1)
  
• 刊名：Human Genetics
• 出版年：2014
• 出版时间：February 2014
• 年：2014
• 卷：133
• 期：2
• 页码：187-197
• 全文大小：925 KB
• 作者单位：Xiaoai Zhang (1)
  Hongmei Xu (2)
  Xiaodan Chen (1) (3)
  Xiujun Li (1) (4)
  Xianjun Wang (5)
  Shujun Ding (5)
  Renli Zhang (6)
  Lijuan Liu (1)
  Cui He (1)
  Lu Zhuang (1)
  Hao Li (1)
  Panhe Zhang (1)
  Hong Yang (1)
  Tingyu Li (2)
  Wei Liu (1)
  Wuchun Cao (1)
  
  1. State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, 20 Dong-Da Street Fengtai District, Beijing, 100071, People’s Republic of China
  2. Children’s Hospital of Chongqing Medical University, Chongqing, 400014, People’s Republic of China
  3. School of Public Health, Central South University, Changsha, 410078, People’s Republic of China
  4. School of Public Health, Shandong University, Jinan, 250012, People’s Republic of China
  5. Shandong Provincial Center for Disease Control and Prevention, Jinan, 250001, People’s Republic of China
  6. Center for Disease Control and Prevention of Shenzhen, Shenzhen, 518020, People’s Republic of China
  
• ISSN：1432-1203

文摘

Myxovirus resistance A (MxA) is an antiviral protein induced by type I interferons α and β (IFN-α and IFN-β) that can inhibit virus replication. We examined whether the MxA polymorphisms were related to the risk and severity of enterovirus 71 (EV71) infection in Chinese populations. The MxA C-123A and G-88T polymorphisms were genotyped in two independent case–control populations in China by polymerase chain reaction-based restriction fragment length polymorphism (PCR-RFLP) analysis. Multivariate logistic regression analysis was used to calculate adjusted odds ratios (ORs) and 95?% confidence intervals (95?% CIs). MxA messenger RNA was quantified by real-time quantitative PCR in peripheral blood mononuclear cells (PBMCs) from 45 healthy children and 19 patients with EV71 infection. Significantly decreased susceptibility to EV71 infection was observed for the -123A allele and -88T allele carriers, with ORs (95?% CIs) estimated as 0.56 (0.39-.81) and 0.64 (0.47-.88), respectively, in the northern population. This association was confirmed in the southern population, with ORs (95?% CIs) estimated as 0.58 (0.38-.89) and 0.67(0.47-.95), respectively. The A- 123T- 88 haplotype was also significantly associated with lower risk of EV71 infection in both the northern (OR?=?0.62; 95?% CI?=?0.44-.85) and the southern population (OR?=?0.63; 95?% CI?=?0.43-.92). Furthermore, we observed higher MxA messenger RNA levels in IFNβ1a-stimulated PBMCs from the -123A or -88T allele carriers compared with that from nocarriers. Our findings suggest that polymorphisms in the MxA promoter may play a role in mediating the susceptibility to EV71 infection in Chinese population.