Influence of hydrophilic amino acids and GC-content on expression of recombinant proteins used in vaccines against foot-and-mouth disease virus in Escherichia coli

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• 作者：Hongfei Wei (1)
  Mingli Fang (1)
  Min Wan (1)
  Hua Wang (1)
  Peiyin Zhang (1)
  Xiaoping Hu (1)
  XiuLi Wu (1)
  Ming Yang (2)
  Yongsheng Zhang (1)
  Lei Zhou (1)
  Chengfeng Jiao (1)
  Li Hua (2)
  Wenzhen Diao (1)
  Yue Xiao (1)
  Yongli Yu (2)
  Liying Wang (1)
  
• 关键词：Epitope ; Escherichia coli ; Foot ; and ; mouth disease virus (FMDV) ; GC ; content ; Hydrophilic amino acids ; Recombinant protein expression
• 刊名：Biotechnology Letters
• 出版年：2014
• 出版时间：April 2014
• 年：2014
• 卷：36
• 期：4
• 页码：723-729
• 全文大小：286 KB
• 参考文献：1. Akashi H, Gojobori T (2002) Metabolic efficiency and amino acid composition in the proteomes of / Escherichia coli and / Bacillus subtilis. Proc Natl Acad Sci USA 99:3695鈥?700 CrossRef
  2. Bonomo J, Gill RT (2005) Amino acid content of recombinant proteins influences the metabolic burden response. Biotechnol Bioeng 90:116鈥?26 CrossRef
  3. Domingo E, Baranowski E, Escarmis C et al (2002) Foot-and-mouth disease virus. Comp Immuno, Microbiol Infect Dis 25:297鈥?08 CrossRef
  4. Fang M, Wang H, Yang M et al (2012) Correlation between efficacy and structure of recombinant epitope vaccines against bovine type O foot and mouth disease virus. Biotechnol Lett 34:839鈥?47 CrossRef
  5. Fowler VL, Bashiruddin JB, Maree FF et al (2011) Foot-and-mouth disease marker vaccine: cattle protection with a partial VP1 G-H loop deleted virus antigen. Vaccine 29:8405鈥?411 CrossRef
  6. Glick BR (1995) Metabolic load and heterologous gene expression. Biotechnol Adv 13:247鈥?61 CrossRef
  7. Han JH, Choi YS, Kim WJ et al (2010) Codon optimization enhances protein expression of human peptide deformylase in / E. coli. Prot Exp Purif 70:224鈥?30 CrossRef
  8. Heizer EM, Raiford DW, Raymer ML et al (2006) Amino acid cost and codon-usage biases in 6 prokaryotic genomes: a whole-genome analysis. Mol Biol Evol 23:1670鈥?680 CrossRef
  9. Ikemura T (1985) Codon usage and tRNA content in unicellular and multicellular organisms. Mol Bid Evol 2:13鈥?4
  10. Jana S, Deb JK (2005) Strategies for efficient production of heterologous proteins in / Escherichia coli. Appl Microbiol Biotechnol 67:289鈥?98 CrossRef
  11. Pedersen S (1984) / Escherichia coli ribosomes translate in vivo with variable rate. EMBO J 12:2895鈥?898
  12. Price WN, Handelman SK, Everett JK et al (2011) Large-scale experimental studies show unexpected amino acid effects on protein expression and solubility in vivo / in E. coli. Microb Inform Exp 1:6. doi:10.1186/2042-5783-1-6
  13. Shao JJ, Wong CK, Lin T et al (2011) Promising multiple-epitope recombinant vaccine against foot-and-mouth disease virus type O in swine. Clin Vaccine Immunol 18:143鈥?49 CrossRef
  14. Sharp PM, Li WH (1987) The codon adaptation index-a measure of directional synonymous codon usage bias, and its potential applications. Nucleic Acid Res 15:1281鈥?295 CrossRef
  15. Sillero A, Ribeiro JM (1989) Isoelectric points of proteins: theoretical determination. Anal Biochem 17:319鈥?25 CrossRef
  16. Sorimachi K (1999) Evolutionary changes reflected by the cellular amino acid composition. Amino Acids 17:207鈥?26 CrossRef
  17. Welch MV, Gustafsson A, Minshull C et al (2011) Designing genes for successful protein expression. Methods Enzymol 498:43鈥?6 CrossRef
  18. Williford A, Demuth JP (2012) Gene expression levels are correlated with synonymous codon usage, amino acid composition, and gene architecture in the Red Flour Beetle, / Tribolium castaneum. Mol Biol Evol 29:3755鈥?766 CrossRef
  
• 作者单位：Hongfei Wei (1)
  Mingli Fang (1)
  Min Wan (1)
  Hua Wang (1)
  Peiyin Zhang (1)
  Xiaoping Hu (1)
  XiuLi Wu (1)
  Ming Yang (2)
  Yongsheng Zhang (1)
  Lei Zhou (1)
  Chengfeng Jiao (1)
  Li Hua (2)
  Wenzhen Diao (1)
  Yue Xiao (1)
  Yongli Yu (2)
  Liying Wang (1)
  
  1. Department of Molecular Biology, Norman Bethune College of Medicine, Jilin University, Changchun, 130021, China
  2. Department of Immunology, Norman Bethune College of Medicine, Jilin University, Changchun, 130021, China
  
• ISSN：1573-6776

文摘

Epitope-based protein expression in Escherichia coli can be improved by adjusting its amino acid composition and encoding genes. To that end, we analyzed 24 recombinant epitope proteins (rEPs) that carry multiple epitopes derived from VP1 protein of foot-and-mouth disease virus. High level expression of the rEPs was attributed to a high content of Arg, Asn, Asp and Thr, a low content of Gln, Pro and Lys, a high content of hydrophilic amino acids and a higher isoelectric point value resulting from abundant Arg. It is also attributed to the appropriate guanine and cytosine content in the encoding genes. The data provide a reference for adjusting the amino acid composition in designing epitope-based proteins used in vaccines and for adjusting the synonymous codons to improve their expressions in E. coli.