Development of a sandwich ELISA for the quantification of enterovirus 71

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• 作者：Shujun Ma (1)
  Qunying Mao (2)
  Zhenglun Liang (2)
  Cuijuan Zhang (1)
  Wenxing Yang (1)
  Zhe Sun (1)
  Haijiang Zhang (1)
  Xinliang Shen (3)
  Shengli Bi (4)
  Le Sun (1)
  
• 关键词：Enterovirus (EV) 71 ; Vaccine ; Sandwich ELISA ; Quantification
• 刊名：Cytotechnology
• 出版年：2014
• 出版时间：May 2014
• 年：2014
• 卷：66
• 期：3
• 页码：413-418
• 全文大小：187 KB
• 参考文献：1. Chua KB, Kasri AR (2011) Hand foot and mouth disease due to Enterovirus 71 in malaysia. Virol Sin 26:221鈥?28 CrossRef
  2. Dong C, Liu L, Zhao H, Wang J, Liao Y, Zhang X, Na R, Liang Y, Wang L, Li Q (2011) Immunoprotection elicited by an Enterovirus type 71 experimental inactivated vaccine in mice and rhesus monkeys. Vaccine 29:6269鈥?275
  3. Infectious Case Report for July 2012 by Ministry of Public Health of China
  4. Kim KH (2009) Enterovirus 71 infection: an experience in Korea. Korean J Pediatr 53(2010):616鈥?22
  5. Li X, Mao C, Ma S, Wang X, Sun Z, Yi Y, Guo M, Shen X, Sun L, Bi S (2009) Generation of neutralizing monoclonal antibodies against Enterovirus 71 using synthetic peptides. Biochem Biophys Res Commun 390:1126鈥?128
  6. Li YP, Liang ZL, Gao Q, Huang LR, Mao QY et al (2012) Safety and immunogenicity of a novel human Enterovirus 71 (EV71) vaccine: a randomized, placebo-controlled, double-blind. Phase I clinical trial. Vaccine 30:3295鈥?303
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  10. Mao Q, Dong C, Li X, Gao Q, Guo Z, Yao X, Wang Y, Gao F, Li F, Xu M, Yin W, Li Q, Shen X, Liang Z, Wang J (2012) Comparative analysis of the immunogenicity and protective effects of inactivated EV71 vaccines in mice. PLoS One 7:e46043 CrossRef
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  12. Tan X, Huang X, Zhu S, Chen H, Yu Q, Wang H, Huo X, Zhou J, Wu Y, Yan D, Zhang Y, Wang D, Cui A, An H, Xu W (2011) The persistent circulation of Enterovirus 71 in People鈥檚 republic of CHINA: causing emerging nationwide epidemics since 2008. PLoS One 6:e25662
  13. Xu WB (2009) Virus Surveillance on HFMD in China. Beijing International Symposium on HFMD, January 13鈥?4, 2009
  
• 作者单位：Shujun Ma (1)
  Qunying Mao (2)
  Zhenglun Liang (2)
  Cuijuan Zhang (1)
  Wenxing Yang (1)
  Zhe Sun (1)
  Haijiang Zhang (1)
  Xinliang Shen (3)
  Shengli Bi (4)
  Le Sun (1)
  
  1. AbMax Biotechnology Co., LTD, 99 Kechuang 14th St,BDA, Beijing, 101111, China
  2. National Institutes for Food and Drug Control, Beijing, 100050, China
  3. Beijing Vigoo Biologicals Co., LTD, 4 South San Jian Fan, Beijing, 100024, China
  4. Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, 100052, China
  
• ISSN：1573-0778

文摘

Since 2008, enterovirus 71 (EV71) has been responsible for high-mortality seasonal epidemics of hand, foot and mouth disease in China. Currently many groups in the world are in the process of developing EV71 vaccines to combat this deadly disease. We have developed three EV71-specific monoclonal antibodies, and in this study we report the establishment of a fast and cost-effective sandwich ELISA kit for measurement of virus concentration in EV71 vaccines using a pair of mouse anti-EV71 monoclonal antibodies. The system is specific for EV71 virus, with no cross-reactivity to coxsackievirus A16, H1N1, rabies, and hepatitis A. Using a reference EV71 vaccine standard, the sensitivity of the assay kit was determined to be 0.82 U/ml, with a linear range between 3.75 and 120 U/ml.