Prediction of peritoneal recurrence by the mRNA level of CEA and MMP-7 in peritoneal lavage of gastric cancer patients

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• 作者：Zhen Li (1)
  Dewei Zhang (1)
  Hao Zhang (2)
  Zhifeng Miao (3)
  Yuanxin Tang (1)
  Gongping Sun (1)
  Dongqiu Dai (1)
  
• 关键词：Gastric cancer ; Tumor marker ; Peritoneal recurrence ; Peritoneal lavage cytologic examination
• 刊名：Tumor Biology
• 出版年：2014
• 出版时间：April 2014
• 年：2014
• 卷：35
• 期：4
• 页码：3463-3470
• 全文大小：193 KB
• 参考文献：1. Marutsuka T, Shimada S, Shiomori K, Hayashi N, Yagi Y, Yamane T, et al. Mechanisms of peritoneal metastasis after operation for non-serosa-invasive gastric carcinoma: an ultrarapid detection system for intraperitoneal free cancer cells and a prophylactic strategy for peritoneal metastasis. Clin Cancer Res. 2003;9:678-5.
  2. Sun Z, Wang ZN, Zhu Z, et al. Evaluation of the seventh edition of American Joint Committee on Cancer TNM staging system for gastric cancer: results from a Chinese monoinstitutional study. Ann Surg Oncol. 2012;19:1918-7. CrossRef
  3. Han TS, Kong SH, Lee HJ, et al. Dissemination of free cancer cells from the gastric lumen and from perigastric lymphovascular pedicles during radical gastric cancer surgery. Ann Surg Oncol. 2011;18:2818-5. CrossRef
  4. Wu B, Wu D, Wang M, Wang G. Recurrence in patients following curative resection of early gastric carcinoma. J Surg Oncol. 2008;98:411-. CrossRef
  5. Glockzin G, Piso P. Current status and future directions in gastric cancer with peritoneal dissemination. Surg Oncol Clin N Am. 2012;21:625-3. CrossRef
  6. Bando E, Yonemura Y, Takeshita Y, Taniguchi K, Yasui T, Yoshimitsu Y, et al. Intraoperative lavage for cytological examination in 1,297 patients with gastric carcinoma. Am J Surg. 1999;178:256-2. CrossRef
  7. Li Z, Miao Z, Jin G, Li X, Li H, Lv Z, et al. βig-h3 supports gastric cancer cell adhesion, migration and proliferation in peritoneal carcinomatosis. Mol Med Rep. 2012;6:558-4.
  8. Katsuragi K, Yashiro M, Sawada T, Osaka H, Ohira M, Hirakawa K. Prognostic impact of PCR-based identification of isolated tumour cells in the peritoneal lavage fluid of gastric cancer patients who underwent a curative R0 resection. Br J Cancer. 2007;97:550-. CrossRef
  9. Ohashi N, Nakanishi H, Kodera Y, et al. Intraoperative quantitative detection of CEA mRNA in the peritoneal lavage of gastric cancer patients with transcription reverse-transcription concerted [TRC] method. A comparative study with real-time quantitative RT-PCR. Anticancer Res. 2007;27:2769-7.
  10. Yonemura Y, Endou Y, Fujimura T, et al. Diagnostic value of preoperative RT-PCR-based screening method to detect carcinoembryonic antigen-expressing free cancer cells in the peritoneal cavity from patients with gastric cancer. ANZ J Surg. 2001;71:521-. CrossRef
  11. Yonemura Y, Fujimura T, Ninomiya I, et al. Prediction of peritoneal micrometastasis by peritoneal lavaged cytology and reverse transcriptase-polymerase chain reaction for matrix metalloproteinase-7 mRNA. Clin Cancer Res. 2001;7:1647-3.
  12. Lv ZD, Na D, Liu FN, et al. Induction of gastric cancer cell adhesion through transforming growth factor-beta-1-mediated peritoneal fibrosis. J Exp Clin Cancer Res. 2010;29:129-9. CrossRef
  13. Yamamoto M, Baba H, Kakeji Y, et al. Prognostic significance of tumor markers in peritoneal lavage in advanced gastric cancer. Oncology. 2004;67:19-6. CrossRef
  14. Miyashiro I, Takachi K, Doki Y, et al. When is curative gastrectomy justified for gastric cancer with positive peritoneal lavage cytology but negative macroscopic peritoneal implant? World J Surg. 2005;29:1131-. CrossRef
  15. Park MR, Lee JH, Park MS, et al. Suppressive effect of 19-nor-1α-25-dihydroxyvitamin D2 on gastric cancer cells and peritoneal metastasis model. J Korean Med Sci. 2012;27:1037-3. CrossRef
  16. Yamauchi H, Suto T, Kigure W, et al. The progression potential of peritoneal dissemination nodules from gastrointestinal tumors. Int Surg. 2011;96:352-. CrossRef
  17. Abe N, Watanabe T, Toda H, et al. Prognostic significance of carcinoembryonic antigen levels in peritoneal washes in patients with gastric cancer. Am J Surg. 2001;181:356-1. CrossRef
  18. Wang JY, Lin SR, Lu CY, et al. Gastric cancer cell detection in peritoneal lavage: RT-PCR for carcinoembryonic antigen transcripts versus the combined cytology with peritoneal carcinoembryonic antigen levels. Cancer Lett. 2005;223:129-5. CrossRef
  19. Nakanishi H, Kodera Y, Yamamura Y, Ito S, Kato T, Ezaki T, et al. Rapid quantitative detection of carcinoembryonic antigen-expressing free tumor cells in the peritoneal cavity of gastric-cancer patients with real-time RT-PCR on the lightcycler. Int J Cancer. 2000;89:411-. CrossRef
  20. Aoyama T, Yoshikawa T, Hayashi T, et al. Risk factors for peritoneal recurrence in stage II/III gastric cancer patients who received S-1 adjuvant chemotherapy after D2 gastrectomy. Ann Surg Oncol. 2012;19:1568-4. CrossRef
  21. Spiliotis J, Halkia E. Slow progress in predicting and preventing fatal gastric cancer peritoneal recurrence. Ann Surg Oncol. 2011;18:S200-. CrossRef
  22. Shu J, Li CG, Liu YC, et al. Comparison of serum tumor associated material (TAM) with conventional biomarkers in cancer patients. Asian Pac J Cancer Prev. 2012;13:2399-03. CrossRef
  23. Pabinger I, Thaler J, Ay C. Biomarkers for prediction of venous thromboembolism in cancer. Blood. 2013;122:2011-. CrossRef
  24. Chen XZ, Zhang WK, Yang K, et al. Correlation between serum CA724 and gastric cancer: multiple analyses based on Chinese population. Mol Biol Rep. 2012;39:9031-. CrossRef
  25. Li GC, Zhang Z, Ma XJ, Gu WL, Wang YN, Li J. Are biomarkers correlated with recurrence patterns in patients with resectable gastric adenocarcinoma. Mol Biol Rep. 2012;39:399-05. CrossRef
  
• 作者单位：Zhen Li (1)
  Dewei Zhang (1)
  Hao Zhang (2)
  Zhifeng Miao (3)
  Yuanxin Tang (1)
  Gongping Sun (1)
  Dongqiu Dai (1)
  
  1. Department of Gastrointestinal Surgery, 4th Affiliated Hospital of China Medical University, Shenyang, Liaoning, 110032, China
  2. Department of Colorectal Surgery, Oncology Hospital of Liaoning Province, Shenyang, China
  3. Department of Surgical Oncology, 1st Affiliated Hospital of China Medical University, Shenyang, China
  
• ISSN：1423-0380

文摘

A number of tumor markers had been reported to be useful in detecting free cancer cells in the peritoneal cavity and predict peritoneal recurrence in gastric cancer patients. The objective of this study was to compare the clinical impact of different tumor markers in peritoneal lavage fluid using the real-time quantitative reverse transcription polymerase chain reaction (RT-PCR) technique and to screen the most effective ones from them. The peritoneal lavage fluid of 116 patients with gastric cancer was sampled at laparotomy. After RNA extraction and reverse transcription, real-time quantitative polymerase chain reaction (PCR) was performed using the primers and probes for carcinoembryonic antigen (CEA), cytokeratin-20, matrix metalloproteinase-7 (MMP-7), carbohydrate antigen 125, and transforming growth factor-beta-1. Among the 116 patients, 45 (38.8?%) were confirmed to have peritoneal recurrence. Any of the PCR-positive results of the five tumor markers could predict peritoneal recurrence in the univariate analysis (P-lt;-.001). In the multivariate analysis, the PCR results of CEA (P--.003) and MMP-7 (P--.028) were found to be independent prognostic factors. A real-time quantitative RT-PCR analysis of the CEA and MMP-7 transcripts in peritoneal lavage fluid could effectively predict peritoneal recurrence in advanced gastric cancer patients who underwent a potentially curative resection.