MiR-495 regulates proliferation and migration in NSCLC by targeting MTA3

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• 作者：Heying Chu (1)
  Xudong Chen (2)
  Huaqi Wang (1)
  Yuwen Du (3)
  Yuanyuan Wang (3)
  Wenqiao Zang (3)
  Ping Li (1)
  Juan Li (1)
  Jingxia Chang (1)
  Guoqiang Zhao (3)
  Guojun Zhang (1)
  
• 关键词：miR ; 495 ; MTA3 ; NSCLC ; Proliferation ; Migration
• 刊名：Tumor Biology
• 出版年：2014
• 出版时间：April 2014
• 年：2014
• 卷：35
• 期：4
• 页码：3487-3494
• 全文大小：2,395 KB
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• 作者单位：Heying Chu (1)
  Xudong Chen (2)
  Huaqi Wang (1)
  Yuwen Du (3)
  Yuanyuan Wang (3)
  Wenqiao Zang (3)
  Ping Li (1)
  Juan Li (1)
  Jingxia Chang (1)
  Guoqiang Zhao (3)
  Guojun Zhang (1)
  
  1. Department of Respiratory Medicine, The First Affiliated Hospital of Zhengzhou University, No.1 Jianshe Road, Zhengzhou, 450052, Henan, China
  2. Department of Histology and Embryology, Luohe Medical College, Luohe, 462002, Henan, China
  3. College of Basic Medical Sciences, Zhengzhou University, No.100 Kexue Road, Zhengzhou, 450001, Henan, China
  
• ISSN：1423-0380

文摘

Our previous studies have showed that metastasis-associated protein 3 (MTA 3) is overexpressed in non-small cell lung cancer (NSCLC) tissue, and increased MTA3 mRNA levels is a risk factor of lymph node metastasis. Using bioinformatics analyses, we found that MTA3 was a potential target of miR-495. However, the pathophysiological role of miR-495 and its relevance to the growth and development of NSCLC have yet to be investigated. The purpose of this study was to elucidate the molecular mechanisms by which miR-495 acts as a tumor suppressor in NSCLC. qRT-PCR data showed significant downregulation of miR-495 in 56 NSCLC tissue samples and 5 lung cancer cell lines, compared with their adjacent normal tissue; furthermore, western blotting analysis revealed MTA3 protein was overexpressed in the tumor samples compared with the matched adjacent normal tissue. MiR-495 was shown to not only inhibit the proliferation of lung cancer cells (A549 and Calu-3) but also to inhibit cell migration in vitro. Using western blotting and luciferase assays, MTA3 was identified as a target of miR-495. These findings suggest the importance of miR-495 targeting of MTA3 in the regulation of lung cancer growth and migration.