SACE_3986, a TetR family transcriptional regulator, negatively controls erythromycin biosynthesis in Saccharopolyspora erythraea

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• 作者：Panpan Wu (1)
  Hui Pan (1)
  Congming Zhang (1)
  Hang Wu (1)
  Li Yuan (1)
  Xunduan Huang (1)
  Ying Zhou (3)
  Bang-ce Ye (3)
  David T. Weaver (1)
  Lixin Zhang (1) (2)
  Buchang Zhang (1)
  
• 关键词：Saccharopolyspora erythraea ; Erythromycin ; TetR family transcriptional regulator ; SACE_3986 ; SACE_3985
• 刊名：Journal of Industrial Microbiology and Biotechnology
• 出版年：2014
• 出版时间：July 2014
• 年：2014
• 卷：41
• 期：7
• 页码：1159-1167
• 全文大小：
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• 作者单位：Panpan Wu (1)
  Hui Pan (1)
  Congming Zhang (1)
  Hang Wu (1)
  Li Yuan (1)
  Xunduan Huang (1)
  Ying Zhou (3)
  Bang-ce Ye (3)
  David T. Weaver (1)
  Lixin Zhang (1) (2)
  Buchang Zhang (1)
  
  1. Institute of Health Sciences, School of Life Sciences, Anhui University, Hefei, 230601, China
  3. State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, 200237, China
  2. CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, China
  
• ISSN：1476-5535

文摘

Erythromycin, a medically important antibiotic, is produced by Saccharopolyspora erythraea. Unusually, the erythromycin biosynthetic gene cluster lacks a regulatory gene, and the regulation of its biosynthesis remains largely unknown. In this study, through gene deletion, complementation and overexpression experiments, we identified a novel TetR family transcriptional regulator SACE_3986 negatively regulating erythromycin biosynthesis in S. erythraea A226. When SACE_3986 was further inactivated in an industrial strain WB, erythromycin A yield of the mutant was increased by 54.2?% in average compared with that of its parent strain, displaying the universality of SACE_3986 as a repressor for erythromycin production in S. erythraea. qRT-PCR analysis indicated that SACE_3986 repressed the transcription of its adjacent gene SACE_3985 (which encodes a short-chain dehydrogenase/reductase), erythromycin biosynthetic gene eryAI and the resistance gene ermE. As determined by EMSA analysis, purified SACE_3986 protein specifically bound to the intergenic region between SACE_3985 and SACE_3986, whereas it did not bind to the promoter regions of eryAI and ermE. Furthermore, overexpression of SACE_3985 in A226 led to enhanced erythromycin A yield by at least 32.6?%. These findings indicate that SACE_3986 is a negative regulator of erythromycin biosynthesis, and the adjacent gene SACE_3985 is one of its target genes. The present study provides a basis to increase erythromycin production by engineering of SACE_3986 and SACE_3985 in S. erythraea.