文摘
Biodegradation of pyridine by a novel bacterial strain, Rhizobium sp. NJUST18, was studied in batch experiments over a wide concentration range (from 100 to 1,000?mg?l?). Pyridine inhibited both growth of Rhizobium sp. NJUST18 and biodegradation of pyridine. The Haldane model could be fitted to the growth kinetics data well with the kinetic constants μ*?=?0.1473?h?, K s?=?793.97?mg?l?, K i?=?268.60?mg?l? and S m?=?461.80?mg?l?. The true μ max, calculated from μ*, was found to be 0.0332?h?. Yield coefficient Y X/S depended on S i and reached a maximum of 0.51?g?g? at S i of 600?mg?l?. V max was calculated by fitting the pyridine consumption data with the Gompertz model. V max increased with initial pyridine concentration up to 14.809?mg?l??h?. The q S values, calculated from \(V_{ \hbox{max} }\) , were fitted with the Haldane equation, yielding q Smax?=?0.1212?g?g??h? and q*?=?0.3874?g?g??h? at S m′??507.83?mg?l?, K s′??558.03?mg?l?, and K i′??462.15?mg?l?. Inhibition constants for growth and degradation rate value were in the same range. Compared with other pyridine degraders, μ max and S m obtained for Rhizobium sp. NJUST18 were relatively high. High K i and K i-values and extremely high K s and K s-values indicated that NJUST18 was able to grow on pyridine within a wide concentration range, especially at relatively high concentrations.