G Protein-Coupled Estrogen Receptor–Protein Kinase A–ERK–CREB Signaling Pathway is Involved in the Regulation of Mouse Gubernaculum Testis Cells by Diethylstilbestrol

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• 作者：Xuan Zhang (1)
  Jian-hong Li (1)
  Shou-xing Duan (1)
  Qing-Jun Lin (1)
  Song Ke (1)
  Lian Ma (2)
  Tian-hua Huang (3)
  Xue-wu Jiang (1)
  
• 刊名：Archives of Environmental Contamination and Toxicology
• 出版年：2014
• 出版时间：July 2014
• 年：2014
• 卷：67
• 期：1
• 页码：97-103
• 全文大小：
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  6. Jiang XW, Li JH, Huang TH, Deng WD (2004) Effect of prenatal exposure to diethylstilbestrol on gubernacular development in fetal male mice. Asian J Androl 6:325-29
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• 作者单位：Xuan Zhang (1)
  Jian-hong Li (1)
  Shou-xing Duan (1)
  Qing-Jun Lin (1)
  Song Ke (1)
  Lian Ma (2)
  Tian-hua Huang (3)
  Xue-wu Jiang (1)
  
  1. Department of Pediatric Surgery, The Second Affiliated Hospital of Shantou University Medical College, Shantou, Guangdong, China
  2. Department of Pediatric, The Second Affiliated Hospital of Shantou University Medical College, Shantou, China
  3. Research Center of Reproductive Medicine, Shantou University Medical College, Shantou, China
  
• ISSN：1432-0703

文摘

The etiology of testicular dysgenesis syndrome is multifactorial and involves environmental factors, such as environmental estrogens. Several studies have shown that hormonal effects on the gubernaculum may affect testicular descent. Diethylstilbestrol (DES) is a nonsteroidal synthetic estrogen that disrupts the morphology and proliferation of gubernacular cells, but the underlying mechanisms remain elusive. In this study, we aimed to determine whether DES may regulate the function of gubernaculum testis cells by way of nongenomic effects mediated by G protein-coupled estrogen receptor (GPER). We used cultured mouse gubernacular testis cells to demonstrate that GPER is expressed in gubernaculum testis cells. Erk1/2 inhibitor PD98059, PKA inhibitor H89, and Src inhibitor PP2 relieved DES-induced inhibition of gubernaculum testis cell proliferation, but ER inhibitor ICI 182780 had no effects on DES-induced inhibition of gubernaculum testis cell proliferation. In addition, we found that DES induced the activation of CREB downstream of PKA, Src, and ERK1/2 in these cells. These data suggest that the effects of DES on mouse gubernaculum testis cells are mediated at least partially by GPER–protein kinase A–ERK–CREB signaling pathway.