Indirect Competitive Enzyme-Linked Immunosorbent Assay for the Detection of Dibutyl Phthalate in White Wine, Compared With GC-MS

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• 作者：Fei Xu (1)
  Wenjun Wang (2)
  Haiyang Jiang (1)
  Zhaopeng Wang (2)
  Zhanhui Wang (1)
  Ping Guo (3)
  Shuangyan Sun (4)
  Shuangyang Ding (1)
  
• 关键词：Dibutyl phthalate ; Polyclonal antibodies ; Enzyme ; linked immunosorbent assay ; White wine ; Gas chromatography–mass spectrometry
• 刊名：Food Analytical Methods
• 出版年：2014
• 出版时间：September 2014
• 年：2014
• 卷：7
• 期：8
• 页码：1619-1626
• 全文大小：218 KB
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• 作者单位：Fei Xu (1)
  Wenjun Wang (2)
  Haiyang Jiang (1)
  Zhaopeng Wang (2)
  Zhanhui Wang (1)
  Ping Guo (3)
  Shuangyan Sun (4)
  Shuangyang Ding (1)
  
  1. Department of Veterinary Pharmacology and Toxicology, College of Veterinary Medicine, China Agricultural University, No.2 Yuanmingyuan West Road, Haidian District, Beijing, 100193, People’s Republic of China
  2. Beijing WDWK Biotechnology Corp., Beijing, 100193, People’s Republic of China
  3. Technology Center of Jiangxi Entry-Exit Inspection and Quarantine Bureau, Nanchang, 330038, People’s Republic of China
  4. Standards and regulation researcher Center of AQSIQ, Beijing, 100028, People’s Republic of China
  
• ISSN：1936-976X

文摘

Plasticizer has attracted more and more attention in China for the past 3?years, especially in Taiwan district. In this study, an indirect competitive enzyme-linked immunosorbent assay (icELISA) has been developed for the determination of a plasticizer dibutyl phthalate (DBP) in white wine. Dibutyl 4-aminophthalate coupled with OVA was synthesized as an immunogen to produce polyclonal antibodies against DBP. The antibody exhibited negligible cross-reactivity with other related compounds. The influence of several physicochemical parameters, such as coating procedure, organic solvent, competitive reaction time, and pH was investigated. The limit of detection was 64.5?ng/mL, which was sensitive enough for a screening assay. The linear range was 64.5-,606.2?ng/mL with a correlation coefficient (R 2) of 0.996. The method was successfully applied to the determination of DBP in white wine. Recoveries were between 83.1 and 101.7?%. This immunoassay was highly specific, sensitive, rapid, simple, and suitable for DBP monitoring. The results obtained were compared with those obtained using gas chromatography–mass spectrometry (GC-MS), and a satisfied correlation coefficient of 0.928 was obtained by real sample detection.