Synergistic anticancer effects of lectin and doxorubicin in breast cancer cells

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• 作者：Chang-Eui Hong (1)
  Ae-Kyung Park (2)
  Su-Yun Lyu (2)
  
• 关键词：Apoptosis ; Breast cancer ; Doxorubicin ; Drug combination ; Mistletoe lectin
• 刊名：Molecular and Cellular Biochemistry
• 出版年：2014
• 出版时间：September 2014
• 年：2014
• 卷：394
• 期：1-2
• 页码：225-235
• 全文大小：1,525 KB
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• 作者单位：Chang-Eui Hong (1)
  Ae-Kyung Park (2)
  Su-Yun Lyu (2)
  
  1. Department of Biology, Chonnam National University, 77 Yongbong-ro, Buk-gu, Gwangju, 500-757, Korea
  2. Department of Pharmacy, Sunchon National University, 255 Jungangno, Suncheon, 540-742, Jeonnam, Korea
  
• ISSN：1573-4919

文摘

We studied the effects, either combined or alone, of lectin from Korean mistletoe (Viscum album var. coloratum agglutinin, VCA) and doxorubicin (DOX) in MCF-7 (estrogen receptor-positive) and MDA-MB231 (estrogen receptor-negative) human breast cancer cells. When VCA and DOX were combined, a strong synergistic effect was shown in cell growth inhibition, compared to VCA or DOX treatment alone. In quantitative apoptosis studies analyzed by flow cytometry, a combination of two agents showed an increase in apoptosis in both cells, compared to agents alone. Also, pro-apoptotic proteins including Bax, Bik, and Puma were increased in both cells, and the survival factor Bcl-2 was inhibited in MCF-7 cells when drugs were combined. Furthermore, VCA combined with DOX mediated S phase arrest, accompanied with a decrease of cell number at G0/G1 phase. This suggests that VCA and DOX combination may possibly lead to a novel strategy for the treatment of breast cancer.