Response of the bacterial diversity and soil enzyme activity in particle-size fractions of Mollisol after different fertilization in a long-term experiment

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• 作者：Ning Ling (1)
  Yuming Sun (1)
  Jinghua Ma (1)
  Junjie Guo (1)
  Ping Zhu (2)
  Chang Peng (2)
  Guanghui Yu (1)
  Wei Ran (1)
  Shiwei Guo (1)
  Qirong Shen (1)
  
• 关键词：Mollisol ; Long ; term experiment ; Soil particle size ; Soil enzyme ; Bacterial community
• 刊名：Biology and Fertility of Soils
• 出版年：2014
• 出版时间：August 2014
• 年：2014
• 卷：50
• 期：6
• 页码：901-911
• 全文大小：1,793 KB
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• 作者单位：Ning Ling (1)
  Yuming Sun (1)
  Jinghua Ma (1)
  Junjie Guo (1)
  Ping Zhu (2)
  Chang Peng (2)
  Guanghui Yu (1)
  Wei Ran (1)
  Shiwei Guo (1)
  Qirong Shen (1)
  
  1. Key Laboratory of Plant Nutrition and Fertilization in Lower-Middle Reaches of the Yangtze River, Ministry of Agriculture, Jiangsu Provincial Coordinated Research Center for Organic Solid Waste Utilization, Nanjing Agricultural University, Nanjing, 210095, China
  2. Agriculture Environment and Resources Center, Jilin Academy of Agricultural Sciences, Jilin, 130033, China
  
• ISSN：1432-0789

文摘

Particle-size soils were fractionated for evaluating changes in the composition of bacterial community and enzyme activity in response to 13?years of fertilization. This study focused on Mollisol and its particle-size fractions of 200-,000?μm (coarse sand sized), 63 to 200?μm (fine sand sized), 2 to 63?μm (silt sized), and 0.1 to 2 to?μm (clay-sized). Long-term chemical fertilization lowered the pH of all particle fractions, whereas organic fertilizer application mitigated soil acidification. Nutrient concentrations depended on both fertilizer treatment and particle fractions and enzymes were unevenly active throughout the soil. Generally, the highest enzyme activities were observed in the silt and clay fractions of control soil and the soil treated with chemical fertilizer (N, P, and K (NPK)) and in the sand-sized fraction of soil treated with manure and chemical fertilizer (MNPK). Except for acid phosphomonoesterase, the other tested enzyme activities in coarse-sized fractions of MNPK soil were significantly higher than those of the control and NPK soils. Fertilization and soil fraction interactively (p--.05) affected the enzyme activity. Denaturing gradient gel electrophoresis analysis showed that the bacterial community structure significantly differed in different particle sizes with a higher bacterial diversity in small-sized than in coarse-sized fractions. Dominant bands were excised and sequenced. We have found the following bacterial groups: Actinobacteria, γ-proteobacteria, and Acidobacteria. In addition, enrichment of organic matter in coarser fractions was related to greater bacterial diversity than any other treatment. Principal component analysis showed a smaller variability among fractions of the organic amended treatment. Redundancy analysis showed that the tested properties significantly affected the composition of bacterial community with the exception of C/N and available P. No significant correlation between enzyme activity and bacterial community composition was detected, whereas positive correlations between other soil properties and enzyme activities were observed to various extents. Probably, enzyme activities might be affected by specific functional bacterial communities rather than by the overall bacterial community. We concluded that the long-term application of organic manures contributed to the increase of soil organic matter content of particles higher than 200?mm, with higher bacterial diversity and increases in most of the enzyme activities.