Isolation and characterization of a cryptogein-like gene from drought and salt-treated Alternanthera philoxeroides roots

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• 作者：Jianming Gao ; Jinzhi Li ; Qiang Xiao ; Caibo Luo ; Shiqing Zhang…
• 关键词：Alligator weed ; Alternanthera philoxeroides ; Cryptogein ; Differential display ; Drought response ; Pichia pastoris ; Salt ; stress response
• 刊名：Biotechnology Letters
• 出版年：2014
• 出版时间：November 2014
• 年：2014
• 卷：36
• 期：11
• 页码：2351-2356
• 全文大小：532 KB
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• 作者单位：Jianming Gao (1)
  Jinzhi Li (2)
  Qiang Xiao (3)
  Caibo Luo (3)
  Shiqing Zhang (1)
  Helong Chen (1)
  Kexian Yi (1)
  
  1. Key Laboratory of Biology and Genetic Resources of Tropical Crops, Ministry of Agriculture, P.R China/Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou, 571101, China
  2. School of Life Science, Taizhou University, Taizhou, 318000, Zhejiang, China
  3. China-UK HUST-RRes Genetic Engineering and Genomics Joint Laboratory, Huazhong University of Science and Technology (HUST), Wuhan, 430074, China
  
• ISSN：1573-6776

文摘

Alligator weed, Alternanthera philoxeroides (Mart.) Grisb, is an amphibious plant with long thick fleshy roots that develop from adventitious roots under drought conditions. To clone differentially-expressed genes from the roots of A. philoxeroides we applied both mRNA differential display and rapid amplification of cDNA ends techniques. A cryptogein-like gene of A. philoxeroides, designated as ApCL, was cloned. On the basis of semi-quantitative RT-PCR analysis results, we demonstrated that the ApCL gene was upregulated under drought and salt stress conditions. After ApCL was transferred to Pichia pastoris GS115 and its resistance to drought and salt then increased by?>100?%. Therefore, the ApCL gene of A. philoxeroides was likely involved in drought and salt tolerance responses.