Upregulated expression of ubiquitin-conjugating enzyme E2Q1 (UBE2Q1) is associated with enhanced cell proliferation and poor prognosis in human hapatocellular carcinoma

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• 作者：Renan Chang (1) (4)
  Lixian Wei (2)
  Yuhua Lu (4)
  Xiaopeng Cui (4)
  Cuihua Lu (2)
  Luoliang Liu (2)
  Dawei Jiang (2)
  YiCheng Xiong (4)
  Gang Wang (4)
  Chunhua Wan (3)
  Haixin Qian (1)
  
• 关键词：Hepatocellular carcinoma ; Ubiquitin ; conjugating enzyme E2Q1 (UBE2Q1) ; Proliferation ; p53 ; Cell cycle
• 刊名：Journal of Molecular Histology
• 出版年：2015
• 出版时间：February 2015
• 年：2015
• 卷：46
• 期：1
• 页码：45-56
• 全文大小：1,744 KB
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• 作者单位：Renan Chang (1) (4)
  Lixian Wei (2)
  Yuhua Lu (4)
  Xiaopeng Cui (4)
  Cuihua Lu (2)
  Luoliang Liu (2)
  Dawei Jiang (2)
  YiCheng Xiong (4)
  Gang Wang (4)
  Chunhua Wan (3)
  Haixin Qian (1)
  
  1. Department of Hepatobiliary Surgery, The First Affiliated Hospital of Soochow University, Suzhou, 215006, China
  4. Department of General Surgery, Affiliated Hospital of Nantong University, Nantong, 226001, China
  2. Department of Digestion, Affiliated Hospital of Nantong University, Nantong, 226001, China
  3. Department of Nutrition and Food Hygiene, School of Public Health, Nantong University, Nantong, 226001, China
  
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Cell Biology
  Biomedicine
  Developmental Biology
  
• 出版者：Springer Netherlands
• ISSN：1567-2387

文摘

Hepatocellular carcinoma (HCC) is the fifth most common cancer in the world. Ubiquitin-proteasome system has been shown to play a pivotal role in the pathophysiology of HCC and other malignancies. UBE2Q1 is a putative E2 ubiquitin conjugating enzyme, and may be involved in the regulation of cancer-related proteins. In this study, we investigated the expression pattern of UBE2Q1 in HCC cell lines and human HCC specimens, and its potential clinical and biological significance in HCC. Western blot and immunohistochemical analyses revealed that UBE2Q1 was significantly upregulated in HCC tumorous tissues compared with the adjacent noncancerous ones. Next, univariate and multivariate survival analyses were performed to determine the prognostic significance of UBE2Q1 in HCC. The results showed that upregulated expression of UBE2Q1 was positively correlated with high histological grades of HCC and predicted poor prognosis. In addition, the expression of UBE2Q1 was progressively increased in serum-refed HCC cells. UBE2Q1 depletion by small interfering RNA inhibited cell proliferation and led to G1 phase arrest in HepG2 and BEL-7404 cells. Furthermore, we showed that cells transfected with UBE2Q1-targeting siRNA resulted in significant increase in the levels of p53, p21 in HepG2 and BEL-7404 cells. These data imply that UBE2Q1 is upregulated in liver cancer cell lines and tumorous samples and may play a role in the development of HCC.