Identification of TaWD40D, a wheat WD40 repeat-containing protein that is associated with plant tolerance to abiotic stresses

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• 作者：Dejing Kong ; Mengjun Li ; Zhanghui Dong ; Hongtao Ji ; Xia Li
• 关键词：Overexpression ; Stress response ; Stress tolerance ; Triticum aestivum ; Virus ; induced gene silencing ; WD40 repeat ; containing protein
• 刊名：Plant Cell Reports
• 出版年：2015
• 出版时间：March 2015
• 年：2015
• 卷：34
• 期：3
• 页码：395-410
• 全文大小：13,399 KB
• 参考文献：1. Adamec L (1984) The effect of plasmolysis and deplasmolysis on the permeability of plant membranes. Biol Plantarum 26:128-31 CrossRef
  2. Ananieva EA, Gillaspy GE, Ely A, Burnette RN, Erickson FL (2008) Interaction of the WD40 domain of a myoinositol polyphosphate 5-phosphatase with SnRK1 links inositol, sugar, and stress signaling. Plant Physiol 148:1868-882 CrossRef
  3. Bray EA (1997) Plant responses to water deficit. Trends Plant Sci 2:48-4 CrossRef
  4. Budak H, Kantar M, Kurtoglu KY (2013) Drought tolerance in modern and wild wheat. Sci World J 2013:548246 CrossRef
  5. Byrt CS, Munns R (2008) Living with salinity. New Phytol 179:903-05 CrossRef
  6. Chinnusamy V, Schumaker K, Zhu JK (2004) Molecular genetic perspectives on cross-talk and specificity in abiotic stress signalling in plants. J Exp Bot 55:225-36 CrossRef
  7. Clough SJ, Bent AF (1998) Floral dip: a simplified method for / Agrobacterium-mediated transformation of / Arabidopsis thaliana. Plant J 16:735-43 CrossRef
  8. Darley CP, van Wuytswinkel OC, van der Woude K, Mager WH, de Boer AH (2000) / Arabidopsis thaliana and / Saccharomyces cerevisiae NHX1 genes encode amiloride sensitive electroneutral Na⁺/H⁺ exchangers. Biochem J 351:241-49 CrossRef
  9. Ekanayake IJ, Dedatta SK, Steponkus PL (1993) Effect of water-deficit stress on diffusive resistance, transpiration, and spikelet desiccation of rice ( / Oryza- / Sativa L). Ann Bot 72:73-0 CrossRef
  10. Ergen NZ, Thimmapuram J, Bohnert HJ, Budak H (2009) Transcriptome pathways unique to dehydration tolerant relatives of modern wheat. Funct Integr Genomics 9:377-96 CrossRef
  11. Finkelstein RR, Somerville CR (1990) Three classes of abscisic acid (ABA)-insensitive mutations of / Arabidopsis define genes that control overlapping subsets of ABA responses. Plant Physiol 94:1172-179 CrossRef
  12. Fujita Y, Fujita M, Satoh R, Maruyama K, Parvez MM, Seki M, Hiratsu K, Ohme-Takagi M, Shinozaki K, Yamaguchi-Shinozaki K (2005) AREB1 is a transcription activator of novel ABRE-dependent ABA signaling that enhances drought stress tolerance in / Arabidopsis. Plant Cell 17:3470-488 CrossRef
  13. Gachomo EW, Jimenez-Lopez JC, Baptiste LJ, Kotchoni SO (2014) GIGANTUS1 (GTS1), a member of Transducin/WD40 protein superfamily, controls seed germination, growth and biomass accumulation through ribosome-biogenesis protein interactions in / Arabidopsis thaliana. BMC Plant Biol 14:37 CrossRef
  14. Haake V, Cook D, Riechmann JL, Pineda O, Thomashow MF, Zhang JZ (2002) Transcription factor CBF4 is a regulator of drought adaptation in / Arabidopsis. Plant Physiol 130:639-48 CrossRef
  15. Hall RA, Lefkowitz RJ (2002) Regulation of G protein-coupled receptor signaling by scaffold proteins. Circ Res 91:672-80 CrossRef
  16. Heinrich R, Neel BG, Rapoport TA (2002) Mathematical models of protein kinase signal transduction. Mol Cell 9:957-70 CrossRef
  17. Himmelbach A, Iten M, Grill E (1998)
• 作者单位：Dejing Kong (1) (2)
  Mengjun Li (1) (3)
  Zhanghui Dong (1) (3)
  Hongtao Ji (1)
  Xia Li (1)
  
  1. The State Key Laboratory of Plant Cell and Chromosome Engineering, Center of Agricultural Resources Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, 286 Huaizhong Road, Shijiazhuang, 050021, Hebei, China
  2. University of Chinese Academy of Sciences, 19A Yuquan Road, Beijing, 100049, China
  3. Shijiazhuang Academy of Agriculture and Forestry Sciences, 479 Shenglibei Street, Shijiazhuang, 050021, Hebei, China
  
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Cell Biology
  Plant Sciences
  Biotechnology
  Plant Biochemistry
  
• 出版者：Springer Berlin / Heidelberg
• ISSN：1432-203X

文摘

Key message TaWD40D that encodes a member of WD40 family proteins is a novel gene involved in the wheat response to abiotic stress. TaWD40D functions as a positive regulator of plant responses to salt stress and osmotic stress in plant. Abstract Abiotic stresses can severely affect plant growth and crop productivity. WD40 repeat-containing proteins play a key role in protein–protein or protein–DNA interactions by acting as scaffolding molecules and promoting protein activity. In this study, a stress-inducible gene, TaWD40D, was identified from Chinese spring wheat (Triticum aestivum L.). TaWD40D encodes a protein containing seven WD40 domains. Subcellular localization in Nicotiana benthamiana mesophyll cells and Arabidopsis root cells showed the presence of TaWD40D in the cytoplasm and nucleus. Heterologous overexpression of TaWD40D in Arabidopsis greatly increased plant tolerance to abscisic acid (ABA), salt stress, and osmotic stress during seed germination and seedling development. The expression patterns of two genes from the SOS pathway (SOS2 and SOS3) and three ABA genes (ABI2, RAB18 and DREB2A) functioning in ABA-dependent and ABA-independent pathways were altered in the transgenic lines overexpressing TaWD40D under the treatments. Notably, the basal level of the ABI2 expression was substantially increased in the TaWD40D overexpression lines. The down-regulation of TaWD40D in wheat by virus-induced gene silencing resulted in a decreased relative water content and less vigorous growth compared to non-silenced lines. Our results suggest that TaWD40D functions as a positive regulator of plant responses to salt stress and osmotic stress that could be utilized for the genetic improvement of stress tolerance in crop plants.