miRNAs play essential roles in the floral thermogenesis of Magnolia denudata (Magnoliaceae)

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• 作者：Xiangyu Liu ; Dechang Cao ; Xiangyu Ji ; Zhixiang Zhang ; Yanwei Wang ; Ruohan Wang
• 关键词：Basal angiosperm ; Floral stage ; Floral thermogenesis ; High ; throughput sequencing ; miRNA
• 刊名：Trees - Structure and Function
• 出版年：2015
• 出版时间：February 2015
• 年：2015
• 卷：29
• 期：1
• 页码：35-42
• 全文大小：1,255 KB
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• 作者单位：Xiangyu Liu (1) (2)
  Dechang Cao (1)
  Xiangyu Ji (1) (2)
  Zhixiang Zhang (2)
  Yanwei Wang (1)
  Ruohan Wang (1)
  
  1. National Engineering Laboratory for Tree Breeding, Key Laboratory for Genetics and Breeding of Forest Trees and Ornamental Plants, Ministry of Education, College of Biological Sciences and Biotechnology, Beijing Forestry University, No. 35 Qinghua East Road, Beijing, 100083, People’s Republic of China
  2. Lab of Systematic Evolution and Biogeography of Woody Plants, College of Nature Conservation, Beijing Forestry University, Beijing, 100083, People’s Republic of China
  
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Forestry
  Plant Sciences
  Agriculture
  Plant Anatomy and Development
  Plant Pathology
  Plant Physiology
  
• 出版者：Springer Berlin / Heidelberg
• ISSN：1432-2285

文摘

Key message High-throughput sequencing and subsequent analysis indicated that miRNAs play crucial roles in floral thermogenesis of Magnolia denudata flowers. Abstract Animal-like thermogenic activity in flowers has been reported in several families of seed plants. Many studies have explored the ecological significance of thermogenesis in pollination biology; however, the molecular mechanisms regulating floral thermogenesis remain unclear. To characterize the roles of miRNA in floral thermogenesis, we analyzed miRNA expression in Magnolia denudata flowers during thermogenic and non-thermogenic stages. High-throughput sequencing and subsequent analysis revealed 82 conserved and 32 novel miRNAs in M. denudata flowers. A total of 187 EST sequences were predicted to be targets of 63 miRNAs. The target genes fell into 15 KOG functional classes and were involved in 25 KEGG pathways, suggesting that miRNAs play extensive regulatory roles in biological processes of M. denudata flowers. Among the identified miRNAs, 17 were differentially expressed between thermogenic and non-thermogenic stages and thus were thought to play roles in regulating floral thermogenesis in M. denudata. GO enrichment analysis revealed that target genes of these thermogenesis-related miRNAs were enriched in the functional groups ‘polyprenyl transferase activity-and ‘photosynthetic electron transport- Considering the important roles of polyprenyl transferase in the respiratory chain and the fact that floral thermogenesis of M. denudata flowers is associated with sunlight, we can infer that miRNAs play crucial roles in floral thermogenesis of M. denudata flowers by regulating cellular respiration and light reactions.