Evaluation of a dansyl-based amino acid DNSBA as an imaging probe for apoptosis detection

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• 作者：Min Tang (1) (2) (3) (4)
  Jiaguo Huang (4) (5)
  Xinxian Weng (1) (2) (3) (4)
  Lifang Yang (1) (2) (3) (4)
  Meihui Liu (4) (5)
  Ming Zhou (4) (5)
  Xiaobo Wang (4) (5)
  Jinghe Gao (1) (2) (3) (4)
  Wei Yi (1) (2) (3) (4)
  Wenbin Zeng (4) (5)
  Lunquan Sun (4) (6)
  Ya Cao (1) (2) (3) (4)
  
• 关键词：Molecular imaging ; Cell apoptosis ; Small molecules probe ; Amino acid ; Detection
• 刊名：Apoptosis
• 出版年：2015
• 出版时间：March 2015
• 年：2015
• 卷：20
• 期：3
• 页码：410-420
• 全文大小：1,195 KB
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• 作者单位：Min Tang (1) (2) (3) (4)
  Jiaguo Huang (4) (5)
  Xinxian Weng (1) (2) (3) (4)
  Lifang Yang (1) (2) (3) (4)
  Meihui Liu (4) (5)
  Ming Zhou (4) (5)
  Xiaobo Wang (4) (5)
  Jinghe Gao (1) (2) (3) (4)
  Wei Yi (1) (2) (3) (4)
  Wenbin Zeng (4) (5)
  Lunquan Sun (4) (6)
  Ya Cao (1) (2) (3) (4)
  
  1. Cancer Research Institute, Xiangya School of Medicine, Central South University, Changsha, 410078, China
  2. Key Laboratory of Carcinogenesis and Invasion, Ministry of Education, Changsha, 410078, China
  3. Key Laboratory of Carcinogenesis, Ministry of Health, Changsha, 410078, China
  4. Molecular Imaging Research Center, Xiangya Hospital, Central South University, Changsha, 410078, China
  5. School of Pharmaceutical Sciences, Central South University, Changsha, 410013, China
  6. Center for Molecular Medicine, Xiangya Hospital, Central South University, Changsha, 410078, China
  
• 刊物类别：Medicine
• 刊物主题：Medicine & Public Health
  Oncology
  Cancer Research
  Cell Biology
  Biochemistry
  Virology
  
• 出版者：Springer Netherlands
• ISSN：1573-675X

文摘

Imaging agents that enable direct detection of apoptosis are highly desirable in the field of monitoring chemotherapeutic response as well as early diagnosis and disease monitoring. Previous work demonstrated that the dansyled amino acid DNSBA is used to specifically and selectively detect apoptotic cancer cells at the both early and late stages, but the mechanism remains unclear. In this work, we evaluated DNSBA as a tool for monitoring cell apoptosis in CNE1 tumor cell models both in vitro and ex vivo after its in vivo administration, which was confirmed by other assays. The ability of DNSBA to detect multiple pathways and different stages of apoptosis leading to cell death may be advantageous in the evaluation of cancer treatment indicative of a positive therapeutic outcome. The uptake change of molecular probes DNSBA in CNE1 cells represented the changes of apoptotic rate in a caspase-dependent manner. However, the accumulation of DNSBA in apoptotic cells did not increase with the enhanced membrane permeability. Furthermore, ex vivo study demonstrated DNSBA has a similar pattern as the TUNEL-positive cells. In conclusion, DNSBA cellular imaging is useful for the early assessment of treatment-induced apoptosis, and thus may act as a substitute for Annexin V for assessing treatment response.