Aerobic and nitrite-dependent methane-oxidizing microorganisms in sediments of freshwater lakes on the Yunnan Plateau

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• 作者：Yong Liu ; Jingxu Zhang ; Lei Zhao ; Yuzhao Li…
• 关键词：Methane ; oxidizing bacteria ; Nitrite ; dependent anaerobic methane oxidation ; High latitude ; Lake ; Sediment
• 刊名：Applied Microbiology and Biotechnology
• 出版年：2015
• 出版时间：March 2015
• 年：2015
• 卷：99
• 期：5
• 页码：2371-2381
• 全文大小：1,262 KB
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• 作者单位：Yong Liu (1)
  Jingxu Zhang (2)
  Lei Zhao (3)
  Yuzhao Li (1)
  Yuyin Yang (2)
  Shuguang Xie (2)
  
  1. Key Laboratory of Water and Sediment Sciences (Ministry of Education), College of Environmental Sciences and Engineering, Peking University, Beijing, 100871, China
  2. State Key Joint Laboratory of Environmental Simulation and Pollution Control, College of Environmental Sciences and Engineering, Peking University, Beijing, 100871, China
  3. Yunnan Key Laboratory of Pollution Process and Management of Plateau Lake-Watershed, Kunming, 650034, China
  
• 刊物类别：Chemistry and Materials Science
• 刊物主题：Chemistry
  Biotechnology
  Microbiology
  Microbial Genetics and Genomics
  
• 出版者：Springer Berlin / Heidelberg
• ISSN：1432-0614

文摘

Both aerobic methane-oxidizing bacteria (MOB) and nitrite-dependent anaerobic methane oxidation (n-damo) bacteria can play an important role in mitigating the methane emission produced in anoxic sediment layers to the atmosphere. However, the environmental factors regulating the distribution of these methane-oxidizing microorganisms in lacustrine ecosystems remain essentially unclear. The present study investigated the distribution of aerobic MOB and n-damo bacteria in sediments of various freshwater lakes on the Yunnan Plateau (China). Quantitative PCR assay and clone library analysis illustrated the spatial variations in the abundances and structures of aerobic MOB and n-damo bacterial communities. Type I MOB (Methylosoma and Methylobacter) and type II MOB (Methylocystis) were detected, while type I MOB was more abundant than type II MOB. Lake sediments n-damo bacterial communities were composed of novel Methylomirabilis oxyfera-like pmoA genes. Lake sediments in the same geographic region could share a relatively similar aerobic MOB community structure. Moreover, Pearson’s correlation analysis indicated that n-damo pmoA gene diversity showed a positive correlation with the ratio of organic matter to total nitrogen in lake sediment.