Simultaneous Detection of Four Common Oral Candida Species from Blood Samples by the Fluorescence Polarization Assay

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• 作者：Yuhong He ; Wei Geng ; Peihuan Wang ; Lanlan Xi…
• 关键词：Candida ; Fluorescence polarization ; Immunoassay ; Oral candidiasis
• 刊名：Cell Biochemistry and Biophysics
• 出版年：2015
• 出版时间：March 2015
• 年：2015
• 卷：71
• 期：2
• 页码：919-924
• 全文大小：218 KB
• 参考文献：1. Coronado-Castellote, L., & Jimenez-Soriano, Y. (2013). Clinical and microbiological diagnosis of oral candidiasis. / Journal of Clinical and Experimental Dentistry, / 5, e279–e286. CrossRef
  2. Neppelenbroek, K. H., Seo, R. S., Urban, V. M., et al. (2014). Identification of / Candida species in the clinical laboratory: A review of conventional, commercial, and molecular techniques. / Oral Diseases, / 20, 329-44. CrossRef
  3. Freydiere, A. M., Guinet, R., & Boiron, P. (2001). Yeast identification in the clinical microbiology laboratory: Phenotypical methods. / Medical Mycology, / 39, 9-3. CrossRef
  4. Guzel, A. B., Ilkit, M., Akar, T., Burgut, R., & Demir, S. C. (2011). Evaluation of risk factors in patients with vulvovaginal candidiasis and the value of chromID Candida agar versus CHROMagar Candida for recovery and presumptive identification of vaginal yeast species. / Medical Mycology, / 49, 16-5. CrossRef
  5. Karageorgopoulos, D. E., Vouloumanou, E. K., Ntziora, F., et al. (2011). Beta-d -glucan assay for the diagnosis of invasive fungal infections: A meta-analysis. / Clinical Infectious Diseases, / 52, 750-70. CrossRef
  6. Lau, A., Sorrell, T. C., Chen, S., et al. (2008). Multiplex tandem PCR: A novel platform for rapid detection and identification of fungal pathogens from blood culture specimens. / Journal of Clinical Microbiology, / 46, 3021-027. CrossRef
  7. Madhavan, P., Jamal, F., Chong, P. P., & Ng, K. P. (2011). Identification of local clinical / Candida isolates using CHROMagar Candida as a primary identification method for various / Candida species. / Tropical Biomedicine, / 28, 269-74.
  8. Sax, P. E., Komarow, L., Finkelman, M. A., et al. (2011). Blood (1,3)-beta-d -glucan as a diagnostic test for HIV-related / Pneumocystis jirovecii pneumonia. / Clinical Infectious Diseases, / 53, 197-02. CrossRef
  9. Spanu, T., Posteraro, B., Fiori, B., et al. (2012). Direct MALDI-TOF mass spectrometry assay of blood culture broths for rapid identification of / Candida species causing bloodstream infections: An observational study in two large microbiology laboratories. / Journal of Clinical Microbiology, / 50, 176-79. CrossRef
  10. Ohiso, I., Tsuruoka, M., Iida, T., Honda, T., & Karube, I. (2000). A fluorescence polarization assay using oligonucleotide probes for the rapid detection of verotoxin-producing / Escherichia coli. / Journal of Biotechnology, / 81, 15-5. CrossRef
  11. Ding, L., Rui, C., Zhansheng, J., et al. (2010). Detection of hepatitis B virus genotypes A to D by the fluorescence polarization assay based on asymmetric PCR. / Journal of Virological Methods, / 168, 31-7. CrossRef
  12. Ju, Z., Yane, G., Ding, L., et al. (2009). Asymmetric GP5+/6+ PCR and hybridization with fluorescence polarization assay of 15 human papillomavirus genotypes in clinical samples. / Journal of Clinical Virology, / 44, 106-10. CrossRef
  13. Meng, Z., Song, R., Chen, Y., et al. (2013). Rapid screening and identification of dominant B cell epitopes of HBV surface antigen by quantum dot-based fluorescence polarization assay. / Nanoscale Research Letters, / 8, 118. CrossRef
  14. Sheng, Y. J., Eremin, S., Mi, T. J., et al. (2014). The development of a fluorescence polarization immunoassay for aflatoxin detection. / Biomedical and Environmental Sciences, / 27, 126-29.
  15. Ying, L., & Xiao-dong, Z. (2013). Identification and susceptibility to antifungal dr
• 刊物主题：Biochemistry, general; Pharmacology/Toxicology; Biotechnology; Cell Biology; Biophysics and Biological Physics;
• 出版者：Springer US
• ISSN：1559-0283

文摘

The genus Candida is both the commensal microbe and the opportunistic pathogen, containing approximately 200 species inhabiting in oral cavity of 53?% of the general population. Candida species can cause the diseases from local mucosal infections to systemic mycoses, even life-threatening infections in immunocompromised individuals. The timely differentiation of Candida species is important for the guidance of clinical medication. Four common Candida species in Chinese population (Candida albicans, Candida tropicalis, Candida glabrata, Candida krusei) were chosen as the targets to develop the rapid screening method in this work. Combined with amplification by asymmetric PCR, this parallel fluorescence polarization (FP) immunoassay is carried out in homogeneous solution phase. The limit of detection of the assay was shown to be 50?copies/mL in blood samples. The evaluation in multicenter manner showed excellent reproducibility and stability. The comparison between DNA sequencing and the FP immunoassay indicated that there was no significant difference between these methods. This molecular strategy-based method is simple, rapid, and feasible for identifying common Candida species and thereby holding great potential in the application of clinical laboratories.