The implications and mechanisms of the extra-nuclear nucleolin in the esophageal squamous cell carcinomas

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• 作者：Jiafeng Qi (1) (2)
  Huiling Li (3)
  Nanbo Liu (1)
  Yutong Xing (2)
  Gang Zhou (2)
  Yao Wu (4)
  Yuanhang Liu (5)
  Wenxia Chen (6)
  Jie Yue (7)
  Bater Han (8)
  Shirong Kang (9)
  Xu Wu (10)
  
  1. Nan Fang Hospital ; Southern Medical University ; Guangzhou ; China
  2. Department of Cardiothoracic Surgery ; The First Affiliated Hospital of Jiamusi University ; Jiamusi ; China
  3. Department of Pediatrics ; The First Affiliated Hospital of Jiamusi University ; Jiamusi ; China
  4. Department of Orthopedics ; The First Hospital of Inner Mongolia Hohhot ; Hohhot ; China
  5. Department of Emergency ; The First Hospital of Inner Mongolia Hohhot ; Hohhot ; China
  6. Department of Infection ; The First Hospital of Inner Mongolia Hohhot ; Hohhot ; China
  7. Department of General Surgery ; Hainan General Hospital ; Hainan ; China
  8. Department of Thoracic Surgery ; People鈥檚 Hospital of Inner Mongolia Medical University ; Hohhot ; China
  9. Department of Thoracic Surgery ; The Affiliated Hospital of Inner Mongolia Medical University ; Hohhot ; China
  10. Department of Cardiothoracic Surgery ; Nan Fang Hospital ; Southern Medical University ; No. 1838 The North of The Guangzhou Road ; Baiyun District ; Guangzhou ; 510515 ; China
  
• 关键词：Nucleolin ; EGFR ; CXCR4 ; Ki67 ; ESCC
• 刊名：Medical Oncology
• 出版年：2015
• 出版时间：March 2015
• 年：2015
• 卷：32
• 期：3
• 全文大小：1,781 KB
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• 刊物主题：Oncology; Hematology; Pathology; Internal Medicine;
• 出版者：Springer US
• ISSN：1559-131X

文摘

In recent decades, the multi-functional protein nucleolin (NCL) has been reported to express outside the nucleus of many cancer cells. However, the expression and role of the extra-nuclear NCL in esophageal squamous cell carcinoma (ESCC) were not well characterized. Here, NCL was detected by immunohistochemistry and Western blotting in 60 ESCC tissues. Further, the associations of NCL, EGFR, CXCR4 and Ki67 were analyzed by in vitro assays. Our results showed that NCL expression in all 40 cases of ESCC tissues with metastasis was extensively located in the nucleus, cytoplasm and cell membrane (extra-nucleus), while NCL expression in all 20 cases of ESCC without metastasis was merely limited into the nucleus (intra-nucleus).The extra-nuclear NCL expression was positively correlated with the expression of EGFR, CXCR4 and Ki67 and serves as an independent prognostic factor for ESCC patients. In vitro, NCL siRNA (si-NCL) efficaciously affected the expression of EGF or SDF-1-induced p-AKT, p-ERK and Ki67. Also, NCL siRNA inhibited the capacity of migration and invasion of ECA109 cells. In conclusions, our study suggests that NCL is implicated in the initiation and transduction of EGFR and CXCR4 signaling and further up-regulates Ki67 expression to modulate the biological behaviors of ESCC. Clinically, the extra-nuclear NCL expression can be used as an important indicator to determine metastasis and predict the prognosis, which help develop new therapeutic strategies against ESCC.