The gene expression profile of porcine alveolar macrophages infected with a highly pathogenic porcine reproductive and respiratory syndrome virus indicates overstimulation of the innate immune system by the virus

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• 作者：Yan Xiao (1)
  Tong-Qing An (1)
  Zhi-Jun Tian (1)
  Tian-Chao Wei (1)
  Yi-Feng Jiang (1)
  Jin-Mei Peng (1)
  Yan-Jun Zhou (1)
  Xue-Hui Cai (1)
  Guang-Zhi Tong (1) (2)
  
  1. State Key Laboratory of Veterinary Biotechnology ; Harbin Veterinary Research Institute ; CAAS ; No. 427 Maduan Street ; Nangang District ; Harbin ; 150001 ; China
  2. Shanghai Veterinary Research Institute ; CAAS ; No. 518 Ziyue Road ; Minhang District ; Shanghai ; 200241 ; China
  
• 刊名：Archives of Virology
• 出版年：2015
• 出版时间：March 2015
• 年：2015
• 卷：160
• 期：3
• 页码：649-662
• 全文大小：3,836 KB
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• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Biomedicine
  Virology
  Medical Microbiology
  Infectious Diseases
  
• 出版者：Springer Wien
• ISSN：1432-8798

文摘

Since the highly pathogenic porcine reproductive and respiratory syndrome virus (HP-PRRSV) variant emerged in 2006, it has caused death in more than 20 million pigs in China and other Southeast Asian countries, making it the most destructive swine pathogen currently in existence. To characterize the cellular responses to HP-PRRSV infection, the gene expression profile of porcine alveolar macrophage (PAM) cells, the primary target cells of PRRSV, was analyzed in HP-PRRSV-infected and uninfected PAMs by suppression subtractive hybridization. After confirmation by Southern blot, genes that were differentially expressed in the HP-PRRSV-infected and uninfected PAMs were sequenced and annotated. Genes that were upregulated mainly in HP-PRRSV-infected PAM cells were related to immunity and cell signaling. Among the differentially expressed genes, Mx1 and HSP70 protein expression was confirmed by western blotting, and IL-8 expression was confirmed by ELISA. In PAM cells isolated from HP-PRRSV-infected piglets, the differential expression of 21 genes, including IL-16, TGF-beta type 1 receptor, epidermal growth factor, MHC-I SLA, Toll-like receptor, hepatoma-derived growth factor, FTH1, and MHC-II SLA-DRB1, was confirmed by real-time PCR. To our knowledge, this is the first study to demonstrate differential gene expression between HP-PRRSV-infected and uninfected PAMs in vivo. The results indicate that HP-PRRSV infection excessively stimulates genes involved in the innate immune response, including proinflammatory cytokines and chemokines.