Astragalus polysaccharide attenuates lipopolysaccharide-induced inflammatory responses in microglial cells: regulation of protein kinase B and nuclear factor-κB signaling

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• 作者：Tao Luo ; Jian Qin ; Min Liu ; Jun Luo ; Fang Ding ; Mingling Wang…
• 关键词：Microglia ; Neuroinflammation ; Astragalus polysaccharide ; Lipopolysaccharide
• 刊名：Inflammation Research
• 出版年：2015
• 出版时间：April 2015
• 年：2015
• 卷：64
• 期：3-4
• 页码：205-212
• 全文大小：861 KB
• 参考文献：1. Kettenmann, H, Hanisch, UK, Noda, M, Verkhratsky, A (2011) Physiology of microglia. Physiol Rev 91: pp. 461-553 ss="external" href="http://dx.doi.org/10.1152/physrev.00011.2010" target="_blank" title="It opens in new window">CrossRef
  2. Cunningham, C (2013) Microglia and neurodegeneration: the role of systemic inflammation. Glia 61: pp. 71-90 ss="external" href="http://dx.doi.org/10.1002/glia.22350" target="_blank" title="It opens in new window">CrossRef
  3. Graeber, MB, Li, W, Rodriguez, ML (2011) Role of microglia in CNS inflammation. FEBS Lett 585: pp. 3798-3805 ss="external" href="http://dx.doi.org/10.1016/j.febslet.2011.08.033" target="_blank" title="It opens in new window">CrossRef
  4. Zhang, Y, Chen, WA (2015) Biochanin A inhibits lipopolysaccharide-induced inflammatory cytokines and mediators production in BV2 microglia. Neurochem Res 40: pp. 165-171 ss="external" href="http://dx.doi.org/10.1007/s11064-014-1480-2" target="_blank" title="It opens in new window">CrossRef
  5. Lee, HJ, Kim, KW (2012) Anti-inflammatory effects of arbutin in lipopolysaccharide-stimulated BV2 microglial cells. Inflamm Res 61: pp. 817-825 ss="external" href="http://dx.doi.org/10.1007/s00011-012-0474-2" target="_blank" title="It opens in new window">CrossRef
  6. Depboylu, C, Stricker, S, Ghobril, JP, Oertel, WH, Priller, J, Hoglinger, GU (2012) Brain-resident microglia predominate over infiltrating myeloid cells in activation, phagocytosis and interaction with T-lymphocytes in the MPTP mouse model of Parkinson disease. Exp Neurol 238: pp. 183-191 ss="external" href="http://dx.doi.org/10.1016/j.expneurol.2012.08.020" target="_blank" title="It opens in new window">CrossRef
  7. Lee, M, McGeer, E, McGeer, PL (2015) Activated human microglia stimulate neuroblastoma cells to upregulate production of beta amyloid protein and tau: implications for Alzheimer’s disease pathogenesis. Neurobiol Aging 36: pp. 42-52 ss="external" href="http://dx.doi.org/10.1016/j.neurobiolaging.2014.07.024" target="_blank" title="It opens in new window">CrossRef
  8. Sanagi, T, Nakamura, Y, Suzuki, E, Uchino, S, Aoki, M, Warita, H (2012) Involvement of activated microglia in increased vulnerability of motoneurons after facial nerve avulsion in presymptomatic amyotrophic lateral sclerosis model rats. Glia 60: pp. 782-793 ss="external" href="http://dx.doi.org/10.1002/glia.22308" target="_blank" title="It opens in new window">CrossRef
  9. Fu, J, Wang, Z, Huang, L, Zheng, S, Wang, D, Chen, S (2014) Review of the botanical characteristics, phytochemistry, and pharmacology of Astragalus membranaceus (Huangqi). Phytother Res 28: pp. 1275-1283 ss="external" href="http://dx.doi.org/10.1002/ptr.5188" target="_blank" title="It opens in new window">CrossRef
  10. Ma, XQ, Shi, Q, Duan, JA, Dong, TT, Tsim, KW (2002) Chemical analysis of Radix astragali (Huangqi) in China: a comparison with its adulterants and seasonal variations. J Agric Food Chem 50: pp. 4861-4866 ss="external" href="http://dx.doi.org/10.1021/jf0202279" target="_blank" title="It opens in new window">CrossRef
  11. Yuan W, Wang J, Wu T. Chinese herbal medicine Huangqi type formulations for nephrotic syndrome. Cochrane Database Syst Rev 2008:CD006335.
  12. Sang, Z, Zhou, L, Fan, X, McCrimmon, RJ (2010) Radix astragali (Huangqi) as a treatment for defective hypoglycemia counter regulation in diabetes. Am J Chin Med 38: pp. 1027-1038 ss="external" href="http://dx.doi.org/10.1142/S0192415X10008445" target="_blank" title="It opens in new window">CrossRef
  13. Liu, C, Wang, G, Chen, G, Mu, Y, Zhang, L, Hu, X (2012) Huangqi decoction inhibits apoptosis and fibrosis, but promotes Kupffer cell activation in dimethylnitrosamine-induced rat liver fibrosis. BMC Complement Altern Med 12: pp. 51 ss="external" href="http://dx.doi.org/10.1186/1472-6882-12-51" target="_blank" title="It opens in new window">CrossRef
  14. Fu, S, Zhang, J, Menniti-Ippolito, F, Gao, X, Galeotti, F, Massari, M (2011) Huangqi injection (a traditional Chinese patent medicine) for chronic heart failure: a systematic review. PLoS One 6: pp. e19604 ss="external" href="http://dx.doi.org/10.1371/journal.pone.0019604" target="_blank" title="It opens in new window">CrossRef
  15. Cheng, XD, Hou, CH, Zhang, XJ, Xie, HY, Zhou, WY, Yang, L (2004) Effects of Huangqi (Hex) on inducing cell differentiation and cell death in K562 and HEL cells. Acta Biochim Biophys Sin (Shanghai) 36: pp. 211-217 ss="external" href="http://dx.doi.org/10.1093/abbs/36.3.211" target="_blank" title="It opens in new window">CrossRef
  16. Cao, J, Chen, Z, Zhu, Y, Li, Y, Guo, C, Gao, K (2014) Huangqi–Honghua combination and its main components ameliorate cerebral infarction with Qi deficiency and blood stasis syndrome by antioxidant action in rats. J Ethnopharmacol 155: pp. 1053-1060
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Biomedicine
  Immunology
  Pharmacology and Toxicology
  Allergology
  Dermatology
  Neurology
  Rheumatology
  
• 出版者：Birkh盲user Basel
• ISSN：1420-908X

文摘

Objectives and design Microglia play an important role in immune and inflammatory responses in the central nervous system. Astragalus polysaccharide (APS) has been reported as an immune stimulant for various inflammation-associated diseases in vivo. The present study investigated the effects of APS on lipopolysaccharide-stimulated inflammatory responses in microglial cells. Materials and methods Cultured BV2 microglial cells were pre-treated with APS (0-00?μg/ml) prior to lipopolysaccharide (50?ng/ml) stimulation. The production of proinflammatory mediators including inducible nitric oxide synthase (iNOS)/nitric oxide (NO), cyclooxygenase-2 (COX-2)/prostaglandin E (PGE2sub>), tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β) were evaluated. Results APS dose-dependently reduced lipopolysaccharide stimulated nitric oxide and PGE2sub> production, as well as iNOS and cyclooxygenase-2 gene expression. It also attenuated proinflammatory cytokines IL-1β and TNF-α generation. In addition, APS inhibited nuclear factor-κB translocation by blockade of IκB degradation and suppressed protein kinase B phosphorylation in lipopolysaccharide-stimulated cells. Conclusions The inhibitory effects of APS on lipopolysaccharide-stimulated inflammatory mediator production in microglia are associated with suppression of nuclear factor-κB and protein kinase B signaling pathways. APS may offer therapeutic potential for treating inflammatory and neurodegenerative diseases accompanied with microglial activation.