Antiviral activities of natural compounds derived from traditional chinese medicines against porcine circovirus type 2 (PCV2)

详细信息    查看全文

• 作者：Na Sun (1)
  Tong Yu (1)
  Jun-Xing Zhao (1)
  Yao-Gui Sun (1)
  Jun-Bing Jiang (1)
  Zhi-Bian Duan (1)
  Wen-Kui Wang (1)
  Yuan-Liang Hu (2)
  Hai-Min Lei (3)
  Hong-Quan Li (1)
  
  1. College of Animal Science and Veterinary Medicine ; Shanxi Agricultural University ; Taigu ; China
  2. Institute of Traditional Chinese Veterinary Medicine ; College of Veterinary Medicine ; Nanjing Agricultural University ; Nanjing ; China
  3. School of Chinese Pharmacy ; Beijing University of Chinese Medicine ; Beijing ; China
  
• 关键词：PCV2 ; matrine ; scutellarin ; antiviral ; in vitro
• 刊名：Biotechnology and Bioprocess Engineering
• 出版年：2015
• 出版时间：February 2015
• 年：2015
• 卷：20
• 期：1
• 页码：180-187
• 全文大小：433 KB
• 参考文献：1. Tischer, I., Gelderblom, H., Vettermann, W., Koch, M. (1982) A very small porcine virus with circular single-stranded DNA. Nature 295: pp. 64-66 CrossRef
  2. Allan, G. M., Ellis, J. A. (2000) Porcine circoviruses: A review. J. Vet. Diagn. Invest. 12: pp. 3-14 CrossRef
  3. Tischer, I., Rasch, R., Tochtermann, G. (1974) Characterization of papovavirus-and picornavirus-like particles in permanent pig kidney cell lines. Zentralbl. Bakteriol. Orig. A. 226: pp. 153-167
  4. Jacobsen, B., Krueger, L., Seeliger, F., Bruegmann, M., Segal茅s, J., Baumgaertner, W. (2009) Retrospective study on the occurrence of porcine circovirus 2 infection and associated entities in Northern Germany. Vet. Microbiol. 138: pp. 27-33 CrossRef
  5. Grau-Roma, L., Fraile, L., Segal茅s, J. (2011) Recent advances in the epidemiology, diagnosis and control of diseases caused by porcine circovirus type 2. Vet. J. 187: pp. 23-32 CrossRef
  6. Segal茅s, J., Kekarainen, T., Cortey, M. (2013) The natural history of porcine circovirus type 2: From an inoffensive virus to a devastating swine disease. Vet. Microbiol. 165: pp. 13-20 CrossRef
  7. Li, T., Peng, T. (2013) Traditional Chinese herbal medicine as a source of molecules with antiviral activity. Antiviral Res. 97: pp. 1-9 CrossRef
  8. Perera, C., Efferth, T. (2012) Antiviral medicinal herbs and phytochemicals. J. Pharmacogn. 3: pp. 45-48
  9. Karuppannan, A. K., Wu, K. X., Qiang, J., Chu, J. J. H., Kwang, J. (2012) Natural compounds inhibiting the replication of Porcine reproductive and respiratory syndrome virus. Antiviral Res. 94: pp. 188-194 CrossRef
  10. Sun, Y., Song, M., Niu, L., Bai, X. Y., Sun, N., Zhao, X., Jiang, J. B., He, J. P., Li, H. Q. (2013) Antiviral effects of the constituents derived from Chinese herb medicines on infectious bursal disease virus. Pharm. Biol. 51: pp. 1137-1143 CrossRef
  11. Wang, Z., Sun, N., Wu, C. H., Jiang, J. B., Bai, Y. S., Li, H. Q. (2013) In vitro antiviral activity and underlying molecular mechanisms of dipotassium glycyrrhetate against porcine reproductive and respiratory syndrome virus. Antivir.Ther. 18: pp. 997-1004 CrossRef
  12. Sun, Y.G., Niu, L., Song, M. Q., Zhao, X., Sun, N., He, J. P., Wu, C. H., Jiang, J. B., Bai, Y. S., Guo, J. H., Li, H. Q. (2014) Screening compounds of Chinese medicinal herbs anti-MDV. Pharm. Biol. 52: pp. 841-847 CrossRef
  13. Zhang, J. Q., Gu, Y. L., Wu, C. H., Ma, H. L., He, J. P., Bai, Y. S., Li, H. Q. (2013) Effects of sodium tanshinone IIA sulfonate against Marek's disease virus in experimentally infected chickens. Int. J. Biol. Macromol. 58: pp. 258-262 CrossRef
  14. Sun, N., Li, E., Wang, Z. W., Zhao, J. X., Wang, S. Y., He, J. P., Bai, Y. S., Li, H. Q. (2014) Sodium tanshinone IIA sulfonate inhibits porcine reproductive and respiratory syndrome virus via suppressing N gene expression and blocking virus induced apoptosis. Antivir. Ther. 19: pp. 89-95 CrossRef
  15. Cheng, J., Sun, N., Zhao, X., Song, M. Q., Jiang, J. B., Bai, Y. S., Li, H. Q. (2013) In vitro screening for compounds derived from traditional chinese medicines with antiviral activities against porcine reproductive and respiratory syndrome virus. J. Microbiol. Biotech. 23: pp. 1076-1083 CrossRef
  16. Sun, N., Wang, Z. W., Wu, C. H., Li, E., He, J. P., Wang, S. Y., Bai, Y. S., Li, H. Q. (2014) Antiviral activity and underlying molecular mechanisms of Matrine against porcine reproductive and respiratory syndrome virus in vitro. Res. Vet. Sci. 96: pp. 323-327 CrossRef
  17. Reed, L. J., Muench, H. (1938) A simple method of estimating fifty per cent endpoints. Am. J. Epidemiol. 27: pp. 493-497
  18. Mosmann, T. (1983) Rapid colorimetric assay for cellular growth and survival: Application to proliferation and cytotoxicity assays. J. Immunol. Methods 65: pp. 55-63 CrossRef
  19. Glatthaar-Saalm眉ller, B., Rauchhaus, U., Rode, S., Haunschild, J., Saalm眉ller, A. (2011) Antiviral activity in vitro of two preparations of the herbal medicinal product Sinupret庐 against viruses causing respiratory infections. Phytomed. 19: pp. 1-7 CrossRef
  20. Fan, Y., Liu, J., Wang, D., Hu, Y., Yang, S., Wang, J., Guo, L., Zhao, X., Wang, H., Jiang, Y. (2011) Epimedium polysaccharide and propolis flavone can synergistically inhibit the cellular infectivity of NDV and improve the curative effect of ND in chicken. Int. J. Biol. Macromol. 48: pp. 439-444 CrossRef
  21. Sun, N., Zhao, X., Bai, X. Y., Niu, L., Song, M. Q., Sun, Y. G., Jiang, J. B., Li, H. Q. (2012) Anti-PRRSV effect and mechanism of sodium tanshinone IIA sulfonate in vitro. J. Asian Nat. Prod. Res. 14: pp. 721-728 CrossRef
  22. Ch谩vez, J.H., Leal, P. C., Yunes, R. A., Nunes, R. J., Barardi, C. R., Pinto, A. R., Sim玫es, C. M., Zanetti, C. R. (2006) Evaluation of antiviral activity of phenolic compounds and derivatives against rabies virus. Vet. Microbiol. 116: pp. 53-59 CrossRef
  23. Tischer, I., Peters, D., Rasch, R., Pociuli, S. (1987) Replication of porcine circovirus: Induction by glucosamine and cell cycle dependence. Arch. Virol. 96: pp. 39-57 CrossRef
  24. Liu, M., Liu, X. Y., Cheng, J. F. (2003) Advance in the pharmacological research on matrine. Zhongguo Zhong Yao Za Zhi 28: pp. 801-804
  25. Ye, G., Zhu, H. Y., Li, Z. X., Ma, C. H., Fan, M. S., Sun, Z. L., Huang, C. G. (2007) LC-MS characterization of efficacy substances in serum of experimental animals treated with Sophora flavescens extracts. Biomed. Chromatogr. 21: pp. 655-660 CrossRef
  26. Cheung, A. K. (2003) Transcriptional analysis of porcine circovirus type 2. Virol. 305: pp. 168-180 CrossRef
  27. Wei, L., Zhu, Z., Wang, J., Liu, J. (2009) JNK and p38 mitogen-activated protein kinase pathways contribute to porcine circovirus type 2 infection. J. Virol. 83: pp. 6039-6047 CrossRef
  28. Zhang, G. H., Wang, Q., Chen, J. J., Zhang, X. M., Tam, S. C., Zheng, Y. T. (2005) The anti-HIV-1 effect of scutellarin. Biochem. Bioph. Res. Co. 334: pp. 812-816 CrossRef
  
• 刊物类别：Chemistry and Materials Science
• 刊物主题：Chemistry
  Biotechnology
  
• 出版者：The Korean Society for Biotechnology and Bioengineering
• ISSN：1976-3816

文摘

Twenty natural compounds isolated from traditional Chinese medicines were examined for their antiviral activities against PCV2 in vitro. Antiviral activity was analyzed by the assays of blocking of virus entry, inhibition of virus replication and inactivation of virus particles in the virus suspension. 50% cytotoxic concentration (CC50) and 50% effective concentration (EC50) for each compound were determined by using the MTT method and immunofluorescence assay (IFA), respectively. The results obtained from the inhibition of virus replication and inactivation of virus particles showed that the maximum inhibition rates for matrine and scutellarin were 57 and 72.69% and their SI were 29.87 and 18.66, respectively. However, the results from the assays of inhibition of virus entry and inhibition of virus replication demonstrated that both aesculetin and arecoline hydrochloride stimulated PCV2 replication, with the maximum stimulation rate of 387.3 and 219.4%. In summary, both matrine and scutellarin had antiviral activity against PCV2, while aesculetin and arecoline hydrochloride promoted PCV2 infection replication in vitro.