The fluorescence enhancement effect of the Tb-Gd-guanosine-5'-triphosphate-phen system and its analytical application

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• 作者：Zongshan Zhao ; Jinghe Yang ; Xia Wu ; Benyu Su ; Changxia Sun and Shufang Liu
• 关键词：Energy transfers ; Fluorescence enhancement ; Guanosine ; 5 ; triphosphate ; Determination ; Rare earth ion
• 刊名：Analytical and Bioanalytical Chemistry
• 出版年：2004
• 出版时间：September 2004
• 年：2004
• 卷：380
• 期：1
• 页码：104-107
• 全文大小：220 KB
• 刊物类别：Chemistry and Materials Science
• 刊物主题：Chemistry
  Analytical Chemistry
  Food Science
  Inorganic Chemistry
  Physical Chemistry
  Monitoring, Environmental Analysis and Environmental Ecotoxicology
  
• 出版者：Springer Berlin / Heidelberg
• ISSN：1618-2650

文摘

It has been found that Tb3+ can react with guanosine-5-triphosphate (GTP) and o-phenanthroline (phen), resulting in the intrinsic fluorescence of Tb3+. This fluorescence can be enhanced by adding La3+, Gd3+, Lu3+, Sc3+, Y3+, and so on, among which Gd3+ produces the greatest enhancement. These are new co-luminescence systems. The experiments indicate under optimum conditions, the fluorescence intensity of the Tb-Gd-GTP-phen system is proportional to the concentration of GTP over the range 1×10–9 to 3×10–5 mol/l. The detection limit is 3.1×10–10 mol/l. The proposed method provides the most sensitive fluorimetry of GTP so far. The mechanism of the Tb-Gd-GTP-phen system has also been studied. The data indicates that there is a large congeries of Tb-Gd-GTP-phen, and the fluorescence enhancement of the Tb-Gd-GTP-phen system is considered to originate from intramolecular and intermocular energy transfers, and the energy-insulating sheath of the Gd complex.