BAC and RNA sequencing reveal the brown planthopper resistance gene BPH15 in a recombination cold spot that mediates a unique defense mechanism

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• 作者：Wentang Lv ; Ba Du ; Xinxin Shangguan ; Yan Zhao ; Yufang Pan ; Lili Zhu…
• 关键词：Brown planthopper ; BPH15 ; Recombination coldspot ; Candidate gene ; RNA sequencing ; Transcriptome ; Defense mechanism
• 刊名：BMC Genomics
• 出版年：2014
• 出版时间：December 2014
• 年：2014
• 卷：15
• 期：1
• 全文大小：1,539 KB
• 参考文献：1. Cheng, X, Zhu, L, He, G (2013) Towards understanding of molecular interactions between rice and the brown planthopper. Mol Plant 6: pp. 621-634 CrossRef
  2. Wang, B, Huang, Z, Shu, L, Ren, X, Li, X, He, G (2001) Mapping of two new brown planthopper resistance genes from wild rice. Chin Sci Bull 46: pp. 1092-1095 CrossRef
  3. Hu, J, Li, X, Wu, C, Yang, C, Hua, H, Gao, G, Xiao, J, He, Y (2012) Pyramiding and evaluation of the brown planthopper resistance genes Bph14 and Bph15 in hybrid rice. Mol Breed 29: pp. 61-69 CrossRef
  4. Li, J, Xia, M, Qi, H, He, G, Wan, B, Zha, Z (2006) Marker-assisted selection for brown planthopper (Nilaparvata lugens St?l) resistance genes Bph14 and Bph15 in rice. Sci Agric Sin 10: pp. 028
  5. Du, B, Zhang, W, Liu, B, Hu, J, Wei, Z, Shi, Z, He, R, Zhu, L, Chen, R, Han, B, He, G (2009) Identification and characterization of Bph14, a gene conferring resistance to brown planthopper in rice. Proc Natl Acad Sci U S A 106: pp. 22163-22168 CrossRef
  6. Hu, J, Cheng, M, Gao, G, Zhang, Q, Xiao, J, He, Y (2013) Pyramiding and evaluation of three dominant brown planthopper resistance genes in the elite indica rice 9311 and its hybrids. Pest Manag Sci 69: pp. 802-808 CrossRef
  7. Yang, H, You, A, Yang, Z, Zhang, F, He, R, Zhu, L, He, G (2004) High-resolution genetic mapping at the Bph15 locus for brown planthopper resistance in rice (Oryza sativa L.). Theor Appl Genet 110: pp. 182-191 CrossRef
  8. Fujita, D, Kohli, A, Horgan, FG (2013) Rice resistance to planthoppers and leafhoppers. Crit Rev Plant Sci 32: pp. 162-191 CrossRef
  9. Walling, LL (2000) The myriad plant responses to herbivores. J Plant Growth Regul 19: pp. 195-216
  10. Walling, LL (2008) Avoiding effective defenses: strategies employed by phloem-feeding insects. Plant Physiol 146: pp. 859-866 CrossRef
  11. Wang, Y, Wang, X, Yuan, H, Chen, R, Zhu, L, He, R, He, G (2007) Responses of two contrasting genotypes of rice to brown planthopper. Mol Plant Microbe Interact 21: pp. 122-132 CrossRef
  12. Wei, Z, Hu, W, Lin, Q, Cheng, X, Tong, M, Zhu, L, Chen, R, He, G (2009) Understanding rice plant resistance to the brown planthopper (Nilaparvata lugens): a proteomic approach. Proteomics 9: pp. 2798-2808 CrossRef
  13. Liu, Y, He, J, Jiang, L, Wu, H, Xiao, Y, Liu, Y, Li, G, Du, Y, Liu, C, Wan, J (2011) Nitric oxide production is associated with response to brown planthopper infestation in rice. J Plant Physiol 168: pp. 739-745 CrossRef
  14. Hao, P, Liu, C, Wang, Y, Chen, R, Tang, M, Du, B, Zhu, L, He, G (2008) Herbivore-induced callose deposition on the sieve plates of rice: an important mechanism for host resistance. Plant Physiol 146: pp. 1810-1820 CrossRef
  15. Wang, Z, Gerstein, M, Snyder, M (2009) RNA-Seq: a revolutionary tool for transcriptomics. Nat Rev Genet 10: pp. 57-63 CrossRef
  16. Wu, J, Mizuno, H, Hayashi-Tsugane, M, Ito, Y, Chiden, Y, Fujisawa, M, Katagiri, S, Saji, S, Yoshiki, S, Karasawa, W, Yoshihara, R, Hayashi, A, Kobayashi, H, Ito, K, Hamada, M, Okamoto, M, Ikeno, M, Ichikawa, Y, Katayose, Y, Yano, M, Matsumoto, T, Sasaki, T (2003) Physical maps and recombination frequency of six rice chromosomes. Plant J 36: pp. 720-730 CrossRef
  17. Kanehisa, M, Goto, S, Kawashima, S, Nakaya, A (2002) The KEGG databases at GenomeNet.
• 刊物主题：Life Sciences, general; Microarrays; Proteomics; Animal Genetics and Genomics; Microbial Genetics and Genomics; Plant Genetics & Genomics;
• 出版者：BioMed Central
• ISSN：1471-2164

文摘

Background Brown planthopper (BPH, Nilaparvata lugens St?l), is the most destructive phloem-feeding insect pest of rice (Oryza sativa). The BPH-resistance gene BPH15 has been proved to be effective in controlling the pest and widely applied in rice breeding programs. Nevertheless, molecular mechanism of the resistance remain unclear. In this study, we narrowed down the position of BPH15 on chromosome 4 and investigated the transcriptome of BPH15 rice after BPH attacked. Results We analyzed 13,000 BC2F2 plants of cross between susceptible rice TN1 and the recombinant inbred line RI93 that carrying the BPH15 gene from original resistant donor B5. BPH15 was mapped to a 0.0269?cM region on chromosome 4, which is 210-kb in the reference genome of Nipponbare. Sequencing bacterial artificial chromosome (BAC) clones that span the BPH15 region revealed that the physical size of BPH15 region in resistant rice B5 is 580-kb, much bigger than the corresponding region in the reference genome of Nipponbare. There were 87 predicted genes in the BPH15 region in resistant rice. The expression profiles of predicted genes were analyzed. Four jacalin-related lectin proteins genes and one LRR protein gene were found constitutively expressed in resistant parent and considered the candidate genes of BPH15. The transcriptomes of resistant BPH15 introgression line and the susceptible recipient line were analyzed using high-throughput RNA sequencing. In total, 2,914 differentially expressed genes (DEGs) were identified. BPH-responsive transcript profiles were distinct between resistant and susceptible plants and between the early stage (6?h after infestation, HAI) and late stage (48 HAI). The key defense mechanism was related to jasmonate signaling, ethylene signaling, receptor kinase, MAPK cascades, Ca2+ signaling, PR genes, transcription factors, and protein posttranslational modifications. Conclusions Our work combined BAC and RNA sequencing to identify candidate genes of BPH15 and revealed the resistance mechanism that it mediated. These results increase our understanding of plant–insect interactions and can be used to protect against this destructive agricultural pest.