Downregulated lincRNA HOTAIR expression in ovarian cancer stem cells decreases its tumorgeniesis and metastasis by inhibiting epithelial-mesenchymal transition

详细信息    查看全文

• 作者：Jing Wang (1)
  Dengyu Chen (2) (3)
  Xiangfeng He (4)
  Yuxia Zhang (1) (2)
  Fangfang Shi (2) (5)
  Di Wu (1) (2)
  Junsong Chen (2)
  Ying Zhang (2)
  Fengsu Zhao (2)
  Jun Dou (2)
  
  1. Department of Gynecology & Obstetrics ; Zhongda Hospital ; School of Medicine ; Southeast University ; Nanjing ; 210009 ; China
  2. Department of Pathogenic Biology and Immunology of School of Medicine ; Southeast University ; Nanjing ; 210009 ; China
  3. Bengbu Medical School ; Department of Microbiology ; Bengbu ; 233030 ; China
  4. Department of Medical Oncology ; Affiliated Tumor Hospital of Nantong University ; Nantong ; 226361 ; China
  5. Department of Oncology ; Zhongda Hospital ; Southeast University ; Nanjing ; 210009 ; China
  
• 关键词：Epithelial ovarian cancer ; Cancer stem cells ; LincRNA HOTAIR ; Epithelial ; mesenchymal transition ; RNA interference
• 刊名：Cancer Cell International
• 出版年：2015
• 出版时间：December 2015
• 年：2015
• 卷：15
• 期：1
• 全文大小：2,900 KB
• 参考文献：1. Bendoraite, A, Knouf, EC, Garg, KS, Parkin, RK, Kroh, EM, O鈥橞riant, KC (2010) Regulation of miR-200 family microRNAs and ZEB transcription factors in ovarian cancer: Evidence supporting a mesothelial-to-epithelial transition. Gynecol Oncol 116: pp. 117-125 6/j.ygyno.2009.08.009" target="_blank" title="It opens in new window">CrossRef
  2. Ho, CM, Chang, SF, Hsiao, CC, Chien, TY, Shih, DT (2012) Isolation and characterization of stromal progenitor cells from ascites of patients with epithelial ovarian adenocarcinoma. J Biomed Sci 19: pp. 23 6/1423-0127-19-23" target="_blank" title="It opens in new window">CrossRef
  3. Cannistra, S-A (2004) Cancer of the ovary. N Eng J Med 351: pp. 2519-2529 6/NEJMra041842" target="_blank" title="It opens in new window">CrossRef
  4. Dou, J, Jiang, CL, Wang, J, Zhang, X, Zhao, F, Hu, W (2011) Using ABCG2- molecule-expressing side population cells to identify cancer stem-like cells in a human ovarian cell line. Cell Biol Int 35: pp. 227-234 CrossRef
  5. Vaibhav, S, Robert, HS (2010) Potential for therapeutic targeting of tumor stem cells. Cancer Sci 101: pp. 16-21 6.2009.01371.x" target="_blank" title="It opens in new window">CrossRef
  6. Chen, D, Zhang, Y, Wang, J, Chen, J, Yang, C, Cai, K (2013) MicroRNA-200c overexpression inhibits tumorigenicity and metastasis of CD117+CD44+ovarian cancer stem cells by regulating epithelial-mesenchymal transition. J Ovarian Res 6: pp. 50 6/1757-2215-6-50" target="_blank" title="It opens in new window">CrossRef
  7. Yang, C, Xiong, F, Wang, J, Dou, J, Chen, J, Chen, D (2014) Anti-ABCG2 monoclonal antibody in combination with paclitaxel-nanoparticles against cancer stem-like cell activity in multiple myeloma. Nanomedicine-(Lond) 9: pp. 45-60 6" target="_blank" title="It opens in new window">CrossRef
  8. John, LR, Kertesz, M, Wang, JK, Squazzo, SL, Xu, X, Brugmann, SA (2007) Functional demarcation of active and silent chromatin domains in human HOX loci by noncoding RNAs. Cell 129: pp. 1311-1323 6/j.cell.2007.05.022" target="_blank" title="It opens in new window">CrossRef
  9. Wilusz, JE, Sunwoo, H, Spector, DL (2009) Long noncoding RNAs: functional surprises from the RNA world. Genes Dev 23: pp. 1494-1504 CrossRef
  10. Gupta, RA, Shah, N, Wang, KC, Kim, J, Horlings, HM, Wong, DJ (2010) Long non-coding RNA HOTAIR reprograms chromatin state to promote cancer metastasis. Nature 464: pp. 1071-1076 CrossRef
  11. Zhang, H, Cai, K, Wang, J, Wang, X, Cheng, K, Shi, F (2014) MiR-7, inhibited by lincRNA HOTAIR, directly inhibites SETDB1 and reverses the EMT of breast cancer stem cells by downregulating the STAT3 pathway. Stem Cell 32: pp. 2858-2868 CrossRef
  12. Geng, YJ, Xie, SL, Li, Q, Ma, J, Wang, GY (2011) Large intervening non-coding RNA HOTAIR is associated with hepatocellular carcinoma progression. J Int Med Res 39: pp. 2119-2128 608" target="_blank" title="It opens in new window">CrossRef
  13. Ishibashi, M, Kogo, R, Shibata, K, Sawada, G, Takahashi, Y, Kurashige, J (2013) Clinical significance of the expression of long non-coding RNA HOTAIR in primary hepatocellular carcinoma. Oncol Rep 29: pp. 946-950
  14. Kogo, R, Shimamura, T, Mimori, K, Kawahara, K, Imoto, S, Sudo, T (2011) Long noncoding RNA HOTAIR regulates polycomb- dependent chromatin modification and is associated with poor prognosis in colorectal cancers. Cancer Res 71: pp. 6320-6326 CrossRef
  15. Kim, K, Jutooru, I, Chadalapaka, G, Johnson, G, Frank, J, Burghardt, R (2013) HOTAIR is a negative prognostic factor and exhibits pro-oncogenic activity in pancreatic cancer. Oncogene 32: pp. 1616-1625 CrossRef
  16. Niinuma, T, Suzuki, H, Nojima, M, Nosho, K, Yamamoto, H, Takamaru, H (2012) Upregulation of miR-196a and HOTAIR drive malignant character in gastrointestinal stromal tumors. Cancer Res 72: pp. 1126-1136 CrossRef
  17. Chen, DY (2013) Study of therapy of ovarian cancer and CSCs by targeted regulating miR-200c-ZEB1 feedback loop. Dissertation For the Academic Degree of Doctor of Medical Science, Southeast University, China
  18. Qiu, JJ, Lin, YY, Ye, LC, Ding, JX, Feng, WW, Jin, HY (2014) Overexpression of long non-coding RNA HOTAIR predicts poor patient prognosis and promotes tumor metastasis in epithelial ovarian cancer. Gynecol Oncol 134: pp. 121-128 6/j.ygyno.2014.03.556" target="_blank" title="It opens in new window">CrossRef
  19. Yang, G, Zhang, S, Gao, F, Liu, Z, Lu, M, Peng, S (1839) Osteopontin enhances the expression of HOTAIR in cancer cells via IRF1. Biochim Biophys Acta 2014: pp. 837-848
  20. Dou, J, Pan, M, Wen, P, Li, Y, Tang, Q, Chu, L (2007) Isolation and identification of cancer stem-like cells from murine melanoma cell lines. Cell Mol Immunol 4: pp. 467-472
  21. Zhang, S, Balch, C, Chan, MW, Lai, HC, Matei, D, Schilder, JM (2008) Identification and characterization of ovarian cancer-initiating cells from primary human tumors. Cancer Res 68: pp. 4311-4320 64" target="_blank" title="It opens in new window">CrossRef
  22. Chen, J, Wang, J, Chen, DY, Yang, J, Yang, C, Zhang, Y (2013) Evaluation of characteristics of CD117鈥?鈥塁D44+ ovarian cancer stem cells in three dimensional basement membrane extract scaffold versus two dimensional monocultures. BMC Cell Biol 14: pp. 7 6/1471-2121-14-7" target="_blank" title="It opens in new window">CrossRef
  23. Arnhold, S, Gl眉er, S, Hartmann, K, Raabe, O, Addicks, K, Wenisch, S (2011) Amniotic-fluid stem cells: growth dynamics and differentiation potential after a CD-117-based selection procedure. Stem Cells Int 23: pp. 715341
  24. Luo, L, Zeng, J, Liang, B, Zhao, Z, Sun, L, Cao, D (2011) Ovarian cancer cells with the CD117 phenotype are highly tumorigenic and are related to chemotherapy outcome. Exp Mol Pathol 91: pp. 596-602 6/j.yexmp.2011.06.005" target="_blank" title="It opens in new window">CrossRef
  25. Choi, YP, Shim, HS, Gao, MQ, Kang, S, Cho, NH (2011) Molecular portraits of intratumoral heterogeneity in human ovarian cancer. Cancer Lett 307: pp. 62-71 6/j.canlet.2011.03.018" target="_blank" title="It opens in new window">CrossRef
  26. Dou, J, He, XF, Liu, Y, Wang, Y, Zhao, F, Wang, X (2014) Effect of downregulation of ZEB1 on vimentin expression, tumour migration and tumourigenicity of melanoma B16F10 cells and CSCs. Cell Biol Int 38: pp. 452-461 CrossRef
  27. Sieh, S, Taubenberger, AV, Rizzi, SC, Sadowski, M, Lehman, ML, Rockstroh, A (2012) Phenotypic characterization of prostate cancer LNCaP cells cultured within a bioengineered microenvironment. PLoS One 7: pp. e40217 CrossRef
  28. He, X, Wang, J, Zhao, F, Yu, F, Chen, D, Cai, K (2012) Antitumor efficacy of viable tumor vaccine modified by heterogenetic ESAT-6 antigen and cytokine IL-21 in melanomatous mouse. Immunol Res 52: pp. 240-249 6-012-8332-4" target="_blank" title="It opens in new window">CrossRef
  29. Shen, B, Chu, ES, Zhao, G, Man, K, Wu, CW, Cheng, JT (2012) PPARgamma inhibits hepatocellular carcinoma metastases in vitro and in mice. Br J Cancer 106: pp. 1486-1494 CrossRef
  30. Ngora, H, Galli, UM, Miyazaki, K, Z枚ller, M (2012) Membrane-bound and exosomal metastasis- associated C44A promotes migration by associating with the 伪(6)尾(4) integrin and MT1-MMP. Neoplasia 14: pp. 95-107
  31. Hu, K, Dou, J, Yu, F, He, X, Yuan, X, Wang, Y (2011) An ocular mucosal administration of nanoparticles containing DNA vaccine pRSC-gD-IL-21 confers protection against mucosal challenge with herpes simplex virus type 1 in mice. Vaccine 29: pp. 1455-1462 6/j.vaccine.2010.12.031" target="_blank" title="It opens in new window">CrossRef
  32. Hu, W, Wang, J, Dou, J, He, X, Zhao, F, Jiang, C (2011) Augmenting therapy of ovarian cancer efficacy by secreting IL-21 human umbilical cord blood stem cells in nude mice. Cell Transplant 20: pp. 669-680 6368910X536509" target="_blank" title="It opens in new window">CrossRef
  33. Zhang, Y, Wang, J, Ren, M, Li, M, Chen, D, Chen, J (2014) Gene therapy of ovarian cancer using IL-21-secreting human umbilical cord mesenchymal stem cells in nude mice. J Ovarian Res 7: pp. 8 6/1757-2215-7-8" target="_blank" title="It opens in new window">CrossRef
  34. Wakahashi, S, Sudo, T, Oka, N, Ueno, S, Yamaguchi, S, Fujiwara, K (2013) VAV1 represses E-cadherin expression through the transactivation of snail and slug: a potential mechanism for aberrant epithelial to mesenchymal transition in human epithelial ovarian cancer. Transl Res 162: pp. 181-190 6/j.trsl.2013.06.005" target="_blank" title="It opens in new window">CrossRef
  35. Luo, X, Dong, Z, Chen, Y, Yang, L, Lai, D (2013) Enrichment of ovarian cancer stem-like cells is associated with epithelial to mesenchymal transition through an miRNA-activated AKT pathway. Cell Prolif 46: pp. 436-446 CrossRef
  36. Khatri, N, Rathi, M, Baradia, D, Trehan, S, Misra, A (2012) In vivo delivery aspects of miRNA, shRNA and siRNA. Crit Rev Ther Drug Carrier Syst 29: pp. 487-527 615/CritRevTherDrugCarrierSyst.v29.i6.20" target="_blank" title="It opens in new window">CrossRef
  
• 刊物主题：Cancer Research; Cell Biology;
• 出版者：BioMed Central
• ISSN：1475-2867

文摘

Background Emerging evidence indicates that dysregulated long intervening non-coding RNA (lincRNA) HOTAIR correlates highly with tumor invasion and metastasis but a link between the high expression of HOTAIR and the metastatic cascade of cancer stem cells (CSCs) needs to be further studied. The purpose of this study was to investigate the effect of down-regulated HOTAIR expression on tumorgeniesis and metastasis of epithelial ovarian cancer (EOC) CSCs. CD117+CD44+CSCs were isolated from human EOC SKOV3 cell line by using a magnetic-activated cell sorting system, and were then transfected with the expression vector-based small hairpin RNA targeting HOTAIR; the stably transfected cells were selected for the study. Colony-forming, wound-healing, cellular metastasis and tumorigenicity assays were performed. Results The results demonstrated that the HOTAIR expression in clinical EOC tissues and SKOV3 CD117+CD44+CSCs was higher than in SKOV3 tumor tissues and non-CD117+CD44+CSCs. The CD117+CD44+-shHOTAIR showed an inhibited HOTAIR expression, reduced cell migration and invasion than CD117+CD44+- scramble, suggesting the inhibition of an epithelial-mesenchymal transition. Moreover, the downregulated HOTAIR expression in CD117+CD44+ CSCs significantly decreased the tumor growth and lung metastasis in xenograft mice. Conclusion Our findings demonstrated the shHOTAIR-mediated down-regulation of the HOTAIR expression in CD117+CD44+ CSCs can be a promising new opportunity for future clinical trials.