Knockdown of a putative alanine aminotransferase gene affects amino acid content and flight capacity in the Colorado potato beetle Leptinotarsa decemlineata

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• 作者：Pin-Jun Wan ; Kai-Yun Fu ; Feng-Gong Lü ; Wen-Chao Guo ; Guo-Qing Li
• 关键词：Leptinotarsa decemlineata ; Alanine aminotransferase ; RNA interference ; Enzyme activity ; Flight ; Mortality
• 刊名：Amino Acids
• 出版年：2015
• 出版时间：July 2015
• 年：2015
• 卷：47
• 期：7
• 页码：1445-1454
• 全文大小：1,460 KB
• 参考文献：Auerswald L, G?de G (1999) The fate of proline in the African fruit beetle Pachnoda sinuata. Insect Biochem Mol Biol 29:687-00View Article
  Baker M, Ferro D, Porter A (2001) Invasions on large and small scales: management of a well-established crop pest, the Colorado potato beetle. Biol Invasions 3:295-06View Article
  Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein–dye binding. Anal Biochem 72:248-54PubMed View Article
  Bursell E (1981) The role of proline in energy metabolism. In: Downer RGH (ed) Energy metabolism in insects. Plenum Press, New York, pp 135-54View Article
  Candy D, Becker A, Wegener G (1997) Coordination and integration of metabolism in insect flight. Comp Biochem Physiol B Biochem Mol Biol 117:497-12View Article
  Chen P, Baker G (1976) l -alanine aminotransferase in the paragonial gland of Drosophila. Insect Biochem 6:441-47View Article
  Darriba D, Taboada GL, Doallo R, Posada D (2011) ProtTest 3: fast selection of best-fit models of protein evolution. Bioinformatics 27:1164-165PubMed View Article
  DiAntonio A (2006) Glutamate receptors at the Drosophila neuromuscular junction. Int Rev Neurobiol 75:165-79PubMed
  Etebari K, Mirhoseini SZ, Matindoost L (2005) A study on intraspecific biodiversity of eight groups of silkworm (Bombyx mori) by biochemical markers. Insect Sci 12:87-4View Article
  Fan J-M, Wan P-J, Fu Q, Li N, Mu L-L, Li G-Q (2014) Molecular cloning and characterization of two alanine aminotransferase genes in the white-backed planthopper Sogatella furcifera. J Asia Pac Entomol 17:355-61View Article
  Felig P (1973) The glucose–alanine cycle. Metabolism 22:179-07PubMed View Article
  G?de G (2004) Flight or fight—the need for adipokinetic hormones. Int Congr Ser 1275:134-40View Article
  G?de G, Auerswald L (2002) Beetles-choice—proline for energy output: control by AKHs. Comp Biochem Physiol Part B 132:117-29View Article
  Giulivi C, Ross-Inta C, Horton A, Luckhart S (2008) Metabolic pathways in Anopheles stephensi mitochondria. Biochem J 415:309-16PubMed View Article
  Inagaki Y, Matsumoto Y, Kataoka K, Matsuhashi N, Sekimizu K (2012) Evaluation of drug-induced tissue injury by measuring alanine aminotransferase (ALT) activity in silkworm hemolymph. BMC Pharmacol Toxicol 13:1-View Article
  Isoe J, Scaraffia PY (2013) Urea synthesis and excretion in Aedes aegypti mosquitoes are regulated by a unique cross-talk mechanism. PLoS One 8:e65393PubMed Central PubMed View Article
  Jadaho SB, Yang RZ, Lin Q, Hu H, Anania FA (2004) Murine alanine aminotransferase: cDNA cloning, functional expression, and differential gene regulation in mouse fatty liver. Hepatology 39:1297-302PubMed View Article
  Khan MA, De Kort CAD (1978) Further evidence for the significance of proline as a substrate for flight in the colorado potato beetle, Leptinotarsa decemlineata. Comp Biochem Physiol B 60:407-11
  Larkin MA, Blackshields G, Brown NP, Chenna R, McGettigan PA, McWilliam H, Valentin F, Wallace IM, Wilm A, Lopez R, Thompson JD, Gibson TJ, Higgins DG (2007) Clustal W and Clustal X version 2.0. Bioinformatics 23:2947-948PubMed View Article
  Liepman AH, Olsen LJ (2003) Alanine aminotransferase homologs catalyze the glutamate:glyoxylate aminotransferase reaction in peroxisomes of Arabidopsis. Plant Physiol 131:215-27PubMed Central PubMed View Article
  Mane SD, Mehrotra K (1977) Alanine aminotransferase: properties and distribution in the desert locust, Schistocerca gregaria. Insect Biochem 7:419-26View Article
  McAllister CH, Facette M, Holt A, Good AG (2013) Analysis of the enzymatic properties of a broad family of alanine aminotransferases. PLoS One 8:e55032PubMed Central PubMed View Article
  Mehta PK, Hale TI, Christen P (1993) Aminotransferases: demonstration of homology and division into evolutionary subgroups. Eur J Biochem 214:549-61PubMed View Article
  Miyashita Y, Dolferus R, Ismond KP, Good AG (2007) Alanine aminotransferase catalyses the breakdown of alanine after hypoxia in Arabidopsis thaliana. Plant J 49:1108-121PubMed View Article
  Mordue W, De Kort C (1978) Energy substrates for flight in the Colorado beetle, Leptinotarsa decemlineata Say. J Insect Physiol 24:221-24View Article
  Noronha C, Cloutier C (1999) Ground and aerial movement of adult Colorado potato beetle (Coleoptera: Chrysomelidae) in a univoltine population. Can Entomol 131:521-38View Article
  Peng L, Schousboe A, Hertz L (1991) Utilization of alpha-ketoglutarate as a precursor for transmitter glutamate in cultured cerebellar granule cells. Neurochem Res 16:29-4PubMed View Article
  Pfaffl MW (2001) A new mathematical model for relative quantification in real-time RT-PCR. Nucleic Acids Res 29:e45PubMed Central PubMed View Article
  Scaraffia P, Wells M (2003) Proline can be utilized as an energy substrate during flight of Aedes aegypti fem
• 作者单位：Pin-Jun Wan (1) (3)
  Kai-Yun Fu (1)
  Feng-Gong Lü (1)
  Wen-Chao Guo (2)
  Guo-Qing Li (1)
  
  1. Education Ministry Key Laboratory of Integrated Management of Crop Diseases and Pests, College of Plant Protection, Nanjing Agricultural University, Nanjing, 210095, China
  3. State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou, 310006, China
  2. Department of Plant Protection, Xinjiang Academy of Agricultural Sciences, ürümqi, 830091, China
  
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Biochemistry
  Analytical Chemistry
  Biochemical Engineering
  Life Sciences
  Proteomics
  Neurobiology
  
• 出版者：Springer Wien
• ISSN：1438-2199

文摘

Alanine aminotransferase (ALT) plays important physiological and biochemical roles in insect. In this study, a full-length Ldalt cDNA was cloned from Leptinotarsa decemlineata. It was ubiquitously expressed in the eggs, larvae, pupae and adults. In the adults, Ldalt mRNA was widely distributed in thorax muscles, fat body, midgut, foregut, hindgut, Malpighian tubules, ventral ganglion and epidermis, with the expression levels from the highest to the lowest. Two double-stranded RNAs (dsRNAs) (dsLdalt1 and dsLdalt2) targeting Ldalt were constructed and bacterially expressed. After adults fed on dsLdalt1- and dsLdalt2-immersed foliage for 3?day, Ldalt mRNA abundance was significantly decreased by 79.5 and 71.1?%, and ALT activities were significantly reduced by 64.5 and 67.6?%, respectively. Moreover, silencing Ldalt affected free amino acid contents. Lysine was decreased by 100.0 and 100.0?%, and arginine was reduced by 87.5 and 89.4?%, respectively, in the hemolymph from dsLdalt1- and dsLdalt2-ingested beetles, compared with control ones. In contrast, proline was increased by 88.7 and 96.4?%. Furthermore, ingestion of dsLdalt1 and dsLdalt2 significantly decreased flight speed, shortened flight duration time and flight distance. In addition, knocking down Ldalt significantly increased adult mortality. These data imply that LdALT plays important roles in amino acid metabolism and in flight in L. decemlineata.