Cytotoxic activities of chemical constituents from rhizomes of Anemarrhena asphodeloides and their analogues

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• 作者：Jing Guo ; Chenghui Xu ; Rui Xue ; Weixin Jiang ; Bin Wu…
• 关键词：Anemarrhena asphodeloides ; Timosaponins ; Xanthones ; Analogues ; Cytotoxic activity
• 刊名：Archives of Pharmacal Research
• 出版年：2015
• 出版时间：May 2015
• 年：2015
• 卷：38
• 期：5
• 页码：598-603
• 全文大小：411 KB
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• 作者单位：Jing Guo (1) (2)
  Chenghui Xu (1)
  Rui Xue (1)
  Weixin Jiang (2)
  Bin Wu (1)
  Chenggang Huang (1)
  
  1. Shanghai Institute of Materia Media, Chinese Academy of Sciences, 501 Haike Road, Zhangjiang, Pudong, Shanghai, 201203, China
  2. Harbin University of Commerce, Harbin, 150076, China
  
• 刊物主题：Pharmacy; Pharmacology/Toxicology;
• 出版者：Springer Netherlands
• ISSN：1976-3786

文摘

Seven steroidal saponins (1-strong class="EmphasisTypeBold">7) and two xanthones (8, 9) were isolated from the rhizomes of Anemarrhena asphodeloides. Then in order to discover more analogues, which may possess good biological activity, the structural modifications of 2 and 9 were performed by acid hydrolysis and acetylation. Consequently, one novel steroidal saponin (2d, timosaponin BII-d), three compounds (2c, 2e and 2f) which were also the new products prepared by the diluted acid hydrolysis of 3 by our group previously, and four known compounds (2a, 2b, 9a and 9b) were obtained. The structures were elucidated by analyses of NMR and MS data. All the compounds were evaluated for their cytotoxicities against BEL-7402, HT-29, HeLa and MDA-MB-468 cell lines in vitro by Sulforhodamine protein coloration method. Compounds 1, 2, 2b, 4-strong class="EmphasisTypeBold">6, 9a and 9b showed certain anti-proliferative activities against the four cell lines, in which compounds 2, 4 and 9b exhibited especially more potent activities. The structure–activity relationships of these compounds were simply discussed.