Recombinant pigment epithelium-derived factor PEDF binds vascular endothelial growth factor receptors 1 and 2

详细信息    查看全文

• 作者：Erin K. Johnston ; Mary K. Francis…
• 关键词：Cell migration ; Endothelial cell ; EPC ; 1 ; PEDF ; VEGF ; VEGFR
• 刊名：In Vitro Cellular & Developmental Biology - Animal
• 出版年：2015
• 出版时间：August 2015
• 年：2015
• 卷：51
• 期：7
• 页码：730-738
• 全文大小：3,546 KB
• 参考文献：Ablonczy Z, Prakasam A, Fant J, Fauq A, Crosson C, Sambamurti K (2009) Pigment epithelium-derived factor maintains retinal pigment epithelium function by inhibiting vascular endothelial growth factor-R2 signaling through gamma-secretase. J Biol Chem 284:30177鈥?0186PubMed Central View Article PubMed
  Anguissola S, McCormack WJ, Morrin MA, Higgins WJ, Fox DM, Worrall DM (2011) Pigment epithelium-derived factor (PEDF) interacts with transportin SR2, and active nuclear import is facilitated by a novel nuclear localization motif. PLoS One 6:e26234PubMed Central View Article PubMed
  Becerra SP, Notario V (2013) The effects of PEDF on cancer biology: mechanisms of action and therapeutic potential. Nat Rev Cancer 13:258鈥?71PubMed Central View Article PubMed
  Becerra SP, Perez-Mediavilla LA, Weldon JE, Locatelli-Hoops S, Senanayake P, Notari L, Notario V, Hollyfield JG (2008) Pigment epithelium-derived factor binds to hyaluronan. Mapping of a hyaluronan binding site. J Biol Chem 283:33310鈥?3320PubMed Central View Article PubMed
  Bernard A, Gao-Li J, Franco CA, Bouceba T, Huet A, Li Z (2009) Laminin receptor involvement in the anti-angiogenic activity of pigment epithelium-derived factor. J Biol Chem 284:10480鈥?0490PubMed Central View Article PubMed
  Cai J, Chen Z, Ruan Q, Han S, Liu L, Qi X, Boye SL, Hauswirth WW, Grant MB, Boulton ME (2011) {gamma}-secretase and presenilin mediate cleavage and phosphorylation of vascular endothelial growth factor receptor-1. J Biol Chem 286:42514鈥?3PubMed Central View Article PubMed
  Cai J, Jiang WG, Grant MB, Boulton M (2006) Pigment epithelium-derived factor inhibits angiogenesis via regulated intracellular proteolysis of vascular endothelial growth factor receptor 1. J Biol Chem 281:3604鈥?613View Article PubMed
  Chen L, Zhang SS, Barnstable CJ, Tombran-Tink J (2006) PEDF induces apoptosis in human endothelial cells by activating p38 MAP kinase dependent cleavage of multiple caspases. Biochem Biophys Res Commun 348:1288鈥?295View Article PubMed
  Chuderland D, Hasky N, Ben-Ami I, Kaplan-Kraicer R, Grossman H, Shalgi R (2013) A physiological approach for treating endometriosis by recombinant pigment epithelium-derived factor (PEDF). Hum Reprod 28:1626鈥?634View Article PubMed
  Craword SE, Fitchev P, Veliceasa D, Volpert OV (2013) The many facets of PEDF in drug discovery and disease: a diamond in the rough or split personality disorder? Expert Opin Drug Discovery 8:769鈥?92View Article
  Dawson DW, Volpert OV, Gillis P, Crawford SE, Xu H, Benedict W, Bouck NP (1999) Pigment epithelium-derived factor: a potent inhibitor of angiogenesis. Science 285:245鈥?48View Article PubMed
  Deshpande M, Notari L, Subramanian P, Notario V, Becerra SP (2012) Inhibition of tumor cell surface ATP synthesis by pigment epithelium-derived factor: implications for antitumor activity. Int J Oncol 41:219鈥?27PubMed Central PubMed
  Doggett DL, Rotenberg MO, Pignolo RJ, Phillips PD, Cristofalo VJ (1992) Differential gene expression between young and senescent, quiescent WI-38 cells. Mech Ageing Dev 65:239鈥?55View Article PubMed
  Duffy KE, Lamb RJ, Mateo LRS, Jordan JL, Canziam G, Brigham-Burke M, Korteweg J, Cunningham M, Beck HS, Carton J et al (2007) Down modulation of human TLR3 function by a monoclonal antibody. Cell Immunol 248:103鈥?14View Article PubMed
  Duh EJ, Yang HS, Suzuma I, Miyagi M, Youngman E, Mori K, Katai M, Yan L, Suzuma K, West K et al (2002) Pigment epithelium-derived factor suppresses ischemia-induced retinal neovascularization and VEGF-induced migration and growth. Invest Ophthalmol Vis Sci 43:821鈥?29PubMed
  Gimbrone MA Jr, Leapman SB, Cotran RS, Folkman J (1972) Tumor dormancy in vivo by prevention of neovascularization. J Exp Med 136:261鈥?76PubMed Central View Article PubMed
  Hanahan D, Folkman J (1996) Patterns and emerging mechanisms of the angiogenic switch during tumorigenesis. Cell 86:353鈥?64View Article PubMed
  Hirsch J, Johnson CL, Nelius T, Kennedy R, Riese W, Filleur S (2011) PEDF inhibits IL8 production in prostate cancer cells through PEDF receptor/phospholipase A2 and regulation of NFkappaB and PPARgamma. Cytokine 55:202鈥?10View Article PubMed
  Ho TC, Chen SL, Shih SC, Chang SJ, Yang SL, Hsieh JW, Cheng HC, Chen LJ, Tsao YP (2011) Pigment epithelium-derived factor (PEDF) promotes tumor cell death by inducing macrophage membrane tumor necrosis factor-related apoptosis-inducing ligand (TRAIL). J Biol Chem 286:35943鈥?5954PubMed Central View Article PubMed
  Ho TC, Chen SL, Yang YC, Liao CL, Cheng HC, Tsao YP (2007) PEDF induces p53-mediated apoptosis through PPAR gamma signaling in human umbilical vein endothelial cells. Cardiovasc Res 76:213鈥?23View Article PubMed
  Hong H, Zhou T, Fang S, Jia M, Xu Z, Dai Z, Li C, Li S, Li L, Zhang T et al (2014) Pigment epithelium-derived factor (PEDF) inhibits breast cancer metastasis by down-regulating fibronectin. Breast Cancer Res Treat 148:61鈥?2View Article PubMed
  Kanda S, Mochizuki Y, Nakamura T, Miyata Y, Matsuyama T, Kanetake H (2005) Pigment epithelium-derived factor inhibits fibroblast-growth-factor-2-induced capillary morphogenesis of endothelial cells through Fyn. J Cell Sci 118:961鈥?70View Article PubMed
  Matsui T, Higashimoto Y, Taira J, Yamagishi S (2013) Pigment epithelium-derived factor (PEDF) binds to caveolin-1 and inhibits the pro-inflammatory effects of caveolin-1 in endothelial cells. Biochem Biophys Res Commun 441:405鈥?10View Article PubMed
  Notari L, Arakaki N, Mueller D, Meier S, Amaral J, Becerra SP (2010) Pigment epithelium-derived factor binds to cell-surface F(1)-ATP synthase. FEBS J 277:2192鈥?205PubMed Central View Article PubMed
  Notari L, Baladron V, Aroca-Aguilar JD, Balko N, Heredia R, Meyer C, Notario PM, Saravanamuthu S, Nueda ML, Sanchez-Sanchez F et al (2006) Identification of a lipase-linked cell membrane receptor for pigment epithelium-derived factor. J Biol Chem 281:38022鈥?8037View Article PubMed
  Notari L, Miller A, Martinez A, Amaral J, Ju M, Robinson G, Smith LE, Becerra SP (2005) Pigment epithelium-derived factor is a substrate for matrix metalloproteinase type 2 and type 9: implications for downregulation in hypoxia. Invest Ophthalmol Vis Sci 46:2736鈥?747View Article PubMed
  Orgaz JL, Benguria A, Sanchez-Martinez C, Ladhani O, Volpert OV, Jimenez B (2011) Changes in the gene expression profile of A375 human melanoma cells induced by overexpression of multifunctional pigment epithelium-derived factor. Melanoma Res 21:285鈥?97PubMed Central View Article PubMed
  Park K, Lee K, Zhang B, Zhou T, He X, Gao G, Murray AR, Ma JX (2011) Identification of a novel inhibitor of the canonical Wnt pathway. Mol Cell Biol 31:3038鈥?051PubMed Central View Article PubMed
  Spranger J, Osterhoff M, Reimann M, Mohlig M, Ristow M, Francis MK, Cristofalo V, Hammes HP, Smith G, Boulton M et al (2001) Loss of the antiangiogenic pigment epithelium-derived factor in patients with angiogenic eye disease. Diabetes 50:2641鈥?645View Article PubMed
  Steele FR, Chader GJ, Johnson LV, Tombran-Tink J (1993) Pigment epithelium-derived factor: neurotrophic activity and identification as a member of the serine protease inhibitor gene family. Proc Natl Acad Sci U S A 90:1526鈥?530PubMed Central View Article PubMed
  Subramanian P, Deshpande M, Locatelli-Hoops S, Moghaddam-Taaheri S, Gutierrez D, Fitzgerald DP, Guerrier S, Rapp M, Notario V, Becerra SP (2012) Identification of pigment epithelium-derived factor protein forms with distinct activities on tumor cell lines. J Biomed Biotechnol 2012:425907PubMed Central PubMed
  Subramanian P, Locatelli-Hoops S, Kenealey J, Desjardin J, Notari L, Becerra SP (2013) Pigment epithelium-derived factor (PEDF) prevents retinal cell death via pigment epithelium-derived factor-R (PEDF-R): identification of a functional ligand binding site. J Biol Chem 288:23928鈥?2PubMed Central View Article PubMed
  Subramanian P, Notario PM, Becerra SP (2010) Pigment epithelium-derived factor receptor (PEDF-R): a plasma membrane-linked phospholipase with PEDF binding affinity. Adv Exp Med Biol 664:29鈥?7PubMed Central View Article PubMed
  Volpert OV, Zaichuk T, Zhou W, Reiher F, Ferguson TA, Stuart PM, Amin M, Bouck NP (2002) Inducer-stimulated Fas targets activated endothelium for destruction by anti-angiogenic thrombospondin-1 and pigment epithelium-derived factor. Nat Med 8:349鈥?57View Article PubMed
  Zhang SX, Wang JJ, Gao G, Parke K, Ma JX (2006) Pigment epithelium-derived factor downregulates vascular endothelial growth factor (VEGF) expression and inhibits VEGF-VEGF receptor 2 binding in diabetic retinopathy. J Mol Endocrinol 37:1鈥?2View Article PubMed
  Zhang Y, Han J, Yang X, Shao C, Xu Z, Cheng R, Cai W, Ma J, Yang Z, Gao G (2011) Pigment epithelium-derived factor inhibits angiogenesis and growth of gastric carcinoma by down-regulation of VEGF. Oncol Rep 26:681鈥?86PubMed
  Zheng Z, Chen H, Zhao H, Liu K, Luo D, Chen Y, Yang X, Gu Q, Xu X (2010) Inhibition of JAK2/STAT3-mediated VEGF upregulation under high glucose conditions by PEDF through a mitochondrial ROS pathway in vitro. Invest Ophthalmol Vis Sci 51:64鈥?1View Article PubMed
  
• 作者单位：Erin K. Johnston (1)
  Mary K. Francis (2)
  Janice E. Knepper (2)
  
  1. Janssen Research and Development, LLC, Pharma R&D Quality and Compliance, 1400 McKean Road, Building 32-12334, Spring House, PA, 19477, USA
  2. Department of Biology, G24A Mendel Science Center, Villanova University, 800 East Lancaster Avenue, Villanova, PA, 19085, USA
  
• 刊物主题：Cell Biology; Developmental Biology; Stem Cells; Cell Culture; Animal Genetics and Genomics;
• 出版者：Springer US
• ISSN：1543-706X

文摘

Angiogenesis, or the formation of new blood vessels, is stimulated by angiogenic factors such as vascular endothelial growth factor (VEGF). Pigment epithelium-derived factor (PEDF) is a potent inhibitor of angiogenesis. To explore the mechanism by which PEDF acts, recombinant PEDF was expressed with a 6x-His tag (for purification) and a green fluorescent protein (GFP) tag. The PEDF fusion protein was confirmed to be active in inhibition of endothelial cell proliferation and migration. Direct binding of PEDF to both vascular endothelial growth factor receptor-1 (VEGFR-1) and VEGFR-2 was demonstrated in an in vitro assay similar to an enzyme-linked immunosorbent assay (ELISA). PEDF was shown by immune-confocal microscopy to be localized within treated endothelial cells. When VEGF-stimulated endothelial cells were incubated with PEDF the VEGF receptors showed intracellular localization. These data suggest that the interaction between PEDF and VEGFR-1 or VEGFR-2 may be a possible mechanism for inhibiting angiogenesis. PEDF may be binding to the VEGF receptors to promote their internalization and/or degradation to limit VEGF responses in treated cells.