Inhibitory effect of totarol on exotoxin proteins hemolysin and enterotoxins secreted by Staphylococcus aureus

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• 作者：Ce Shi ; Xingchen Zhao ; Wenli Li ; Rizeng Meng…
• 关键词：Staphylococcus aureus ; Totarol ; Virulence factors ; Infection ; Staphylococcal enterotoxins
• 刊名：World Journal of Microbiology & Biotechnology
• 出版年：2015
• 出版时间：October 2015
• 年：2015
• 卷：31
• 期：10
• 页码：1565-1573
• 全文大小：1,263 KB
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• 作者单位：Ce Shi (1) <br> Xingchen Zhao (1) <br> Wenli Li (1) <br> Rizeng Meng (2) <br> Zonghui Liu (1) <br> Mingyuan Liu (3) <br> Na Guo (1) <br> Lu Yu (1) <br><br>1. Department of Food Quality and Safety, Jilin University, Changchun, 130062, People’s Republic of China <br> 2. Jilin Entry-Exit Inspection and Quarantine Bureau, Changchun, 130062, People’s Republic of China <br> 3. Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, 225009, People’s Republic of China <br>
• 刊物类别：Chemistry and Materials Science
• 刊物主题：Chemistry<br>Applied Microbiology<br>Biotechnology<br>Biochemistry<br>Environmental Biotechnology<br>Microbiology<br>
• 出版者：Springer Netherlands
• ISSN：1573-0972

文摘

Staphylococcus aureus (S. aureus) causes a wide variety of infections, which are of major concern worldwide. S. aureus produces multiple virulence factors, resulting in food infection and poisoning. These virulence factors include hyaluronidases, proteases, coagulases, lipases, deoxyribonucleases and enterotoxins. Among the extracellular proteins produced by S. aureus that contribute to pathogenicity, the exotoxins α-hemolysin, staphylococcal enterotoxin A (SEA) and staphylococcal enterotoxin B (SEB) are thought to be of major significance. Totarol, a plant extract, has been revealed to inhibit the proliferation of several pathogens effectively. However, there are no reports on the effects of totarol on the production of α-hemolysin, SEA or SEB secreted by S. aureus. The aim of this study was to evaluate the effects of totarol on these three exotoxins. Hemolysis assay, western blotting and real-time reverse transcriptase-PCR assay were performed to identify the influence of graded subinhibitory concentrations of totarol on the production of α-hemolysin and the two major enterotoxins, SEA and SEB, by S. aureus in a dose-dependent manner. Moreover, an enzyme linked immunosorbent assay showed that the TNF-α production of RAW264.7 cells stimulated by S. aureus supernatants was inhibited by subinhibitory concentrations of totarol. Form the data, we propose that totarol could potentially be used as a promising natural compound in the food and pharmaceutical industries. Keywords Staphylococcus aureus Totarol Virulence factors Infection Staphylococcal enterotoxins