Identification of Novel Inflammatory Cytokines and Contribution of Keratinocyte-Derived Chemokine to Inflammation in Response to Vibrio vulnificus Infection in Mice

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• 作者：Xiao-Fei Liu ; Jing Wu ; Ming-Yi Wang ; Ying-Jian Chen ; Yuan Cao ; Cheng-Jin Hu
• 关键词：Vibrio vulnificus infection ; inflammation ; cytokine ; keratinocyte ; derived chemokine
• 刊名：Inflammation
• 出版年：2015
• 出版时间：October 2015
• 年：2015
• 卷：38
• 期：5
• 页码：1864-1873
• 全文大小：1,714 KB
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• 作者单位：Xiao-Fei Liu (1)
  Jing Wu (1)
  Ming-Yi Wang (2)
  Ying-Jian Chen (1)
  Yuan Cao (1)
  Cheng-Jin Hu (1)
  
  1. Department of Laboratory Medicine, General Hospital of Ji’nan Military Region of PLA, Ji’nan, 250031, Shandong Province, People’s Republic of China
  2. Department of Clinical Laboratory, Weihai Municipal Hospital affiliated to Dalian Medical University, Weihai, 264200, Shandong Province, People’s Republic of China
  
• 刊物类别：Medicine
• 刊物主题：Medicine & Public Health
  Rheumatology
  Internal Medicine
  Pharmacology and Toxicology
  Pathology
  
• 出版者：Springer Netherlands
• ISSN：1573-2576

文摘

Currently, only tumor necrosis factor alpha (TNF-α) and interleukin family cytokines have been found to be elicited in Vibrio vulnificus (V. vulnificus)-infected animal models and humans. However, multiple other cytokines are also involved in the immune and inflammatory responses to foreign microorganism infection. Antibody array technology, unlike traditional enzyme-linked immunosorbent assay (ELISA), is able to detect multiple cytokines at one time. Therefore, in this study, we examined the proinflammatory cytokine profile in the serum and liver homogenate samples of bacterial-infected mice using antibody array technology. We identified nine novel cytokines in response to V. vulnificus infection in mice. We found that keratinocyte-derived chemokine (KC) was the most elevated cytokine and demonstrated that KC played a very important role in the V. vulnificus infection-elicited inflammatory response in mice, as evidenced by the fact that the blocking of KC by anti-KC antibody reduced hepatic injury in vivo and that KC induced by V. vulnificus infection in AML-12 cells chemoattracted neutrophils. Our findings implicate that KC may serve as a novel diagnostic biomarker and a possible therapeutic target for V. vulnificus infection. KEY WORDS Vibrio vulnificus infection inflammation cytokine keratinocyte-derived chemokine