Sphingobacterium rhinocerotis sp. nov., isolated from the faeces of Rhinoceros unicornis

详细信息    查看全文

• 作者：Gui-Ding Li ; Xiu Chen ; Qin-Yuan Li ; Fang-Ji Xu ; Shu-Mei Qiu…
• 关键词：Sphingobacterium rhinocerotis sp. nov. ; Faeces ; Rhinoceros unicornis
• 刊名：Antonie van Leeuwenhoek
• 出版年：2015
• 出版时间：November 2015
• 年：2015
• 卷：108
• 期：5
• 页码：1099-1105
• 全文大小：446 KB
• 参考文献：Cerny G (1978) Studies on aminopeptidase for the distinction of Gram-negative from Gram-positive bacteria. Appl Microbiol Biotechnol 5:113-22CrossRef
  Choi HA, Lee SS (2012) Sphingobacterium?kyonggiense?sp. nov., isolated from chloroethene-contaminated soil, and emended descriptions of sphingobacterium daejeonense and Sphingobacterium?mizutaii. Int J Syst Evol Microbiol 62:2559-564CrossRef PubMed
  Christensen H, Angen O, Mutters R, Olsen JE, Bisgaard M (2000) DNA–DNA hybridization determined in microwells using covalent attachment of DNA. Int J Syst Evol Microbiol 50:1095-102CrossRef PubMed
  Collins MD, Jones D (1980) Lipids in the classification and identification of coryneform bacteria containing peptidoglycans based on 2,4-diaminobutyric acid. J Appl Bacteriol 48:459-70CrossRef
  Collins MD, Pirouz T, Goodfellow M, Minnikin DE (1977) Distribution of menaquinones in actinomycetes and corynebacteria. J Gen Microbiol 100:221-30CrossRef PubMed
  Duan SW, Liu ZC, Feng XY, Zheng K, Cheng LF (2009) Sphingobacterium bambusae sp. nov., isolated from soil of bamboo plantation. J Microbiol 47:693-98CrossRef PubMed
  Ezaki T, Hashimoto Y, Yabuuchi E (1989) Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39:224-29CrossRef
  Felsenstein J (1981) Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17:368-76CrossRef PubMed
  Felsenstein J (1985) Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783-91CrossRef
  Fitch WM (1971) Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 20:406-16CrossRef
  He L, Li W, Huang Y, Wang LM, Liu ZH, Lanoot BJ, Vancanneyt M, Swings J (2005) Streptomyces jietaisiensis sp. nov., isolated from soil in northern China. Int J Syst Evol Microbiol 55:1939-944CrossRef PubMed
  He X, Xiao T, Kuang H, Lan X, Tudahong M, Osman G, Fang C, Rahman E (2010) Sphingobacterium?shayense?sp. nov., isolated from forest soil. Int J Syst Evol Microbiol 60:2377-381CrossRef PubMed
  Holmes B, Owen RJ, Hollis DG (1982) Flavobacterium spiritivorum, a new species isolated from human clinical specimens. Int J Syst Bacteriol 32:157-65CrossRef
  Hsu SC, Lockwood JL (1975) Powdered chitin agar as selective medium for enumeration of actinomycetes in water and soil. Appl Microbiol 29:422-26PubMed Central PubMed
  Kim OS, Cho YJ, Lee K, Yoon SH, Kim M, Na H, Park SC, Jeon YS, Lee JH, Yi H, Won S, Chun J (2012) Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 62:716-21CrossRef PubMed
  Kimura M (1980) A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16:111-20CrossRef PubMed
  Kroppenstedt RM (1982) Separation of bacterial menaquinones by HPLC using reverse phase (RP18) and a silver loaded ion exchanger as stationary phases. J Liq Chromatogr 5:2359-387CrossRef
  Lee DH, Hur JS, Kahng HY (2013) Sphingobacterium?cladoniae sp. nov., isolated from lichen, Cladonia sp., and emended description of Sphingobacterium?siyangense. Int J Syst Evol Microbiol 63:755-60CrossRef PubMed
  Leifson E (1960) Atlas of bacterial flagellation. Academic Press, LondonCrossRef
  Li WJ, Xu P, Schumann P, Zhang YQ, Pukall R, Xu LH, Stackebrandt E, Jiang CL (2007) Georgenia?ruanii sp. nov., a novel actinobacterium isolated from forest soil in Yunnan (China) and emended description of the genus Georgenia. Int J Syst Evol Microbiol 57:1424-428CrossRef PubMed
  Liu J, Yang LL, Xu CK, Xi JQ, Yang FX, Zhou F, Zhou Y, Mo MH, Li WJ (2012) Sphingobacterium?nematocida sp. nov., a nematicidal endophytic bacterium isolated from tobacco. Int J Syst Evol Microbiol 62:1809-813CrossRef PubMed
  Matsuyama H, Katoh H, Ohkushi T, Satoh A, Kawahara K, Yumoto I (2008) Sphingobacterium?kitahiroshimense?sp. nov., isolated from soil. Int J Syst Evol Microbiol 58:1576-579CrossRef PubMed
  Mehnaz S, Weselowski B, Lazarovits G (2007) Sphingobacterium?canadense sp. nov., an isolate from corn roots. Syst Appl Microbiol 30:519-24CrossRef PubMed
  Mesbah M, Premachandran U, Whitman WB (1989) Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39:159-67CrossRef
  Minnikin DE, Collins MD, Goodfellow M (1979) Fatty acid and polar lipid composition in the classification of Cellulomonas, Oerskovia and related taxa. J Appl Bacteriol 47:87-5CrossRef
  Saitou N, Nei M (1987) The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406-25PubMed
  Sasser M (1990) Identification of bacteria by gas chromatography o
• 作者单位：Gui-Ding Li (1)
  Xiu Chen (2)
  Qin-Yuan Li (1) (3)
  Fang-Ji Xu (1)
  Shu-Mei Qiu (4)
  Yi Jiang (1)
  Cheng-Lin Jiang (1)
  
  1. Yunnan Institute of Microbiology, School of Life Sciences, Yunnan University, Kunming, 650091, People’s Republic of China
  2. Institute of Microbial Pharmaceuticals, College of Life and Health Sciences, Northeastern University, Shenyang, 110819, People’s Republic of China
  3. Life Sciences Lab Center, School of Life Sciences, Yunnan University, Kunming, 650091, People’s Republic of China
  4. Yunnan Wild Animal Park, Kunming, 650218, People’s Republic of China
  
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Microbiology
  Medical Microbiology
  Plant Sciences
  Soil Science and Conservation
  
• 出版者：Springer Netherlands
• ISSN：1572-9699

文摘

A novel Gram-negative, strictly aerobic, short rod-shaped, non-motile bacterium, designated YIM 101302T, was isolated from the faeces of Rhinoceros unicornis dwelling in the Yunnan Wild Animal Park, Yunnan province, South-West China. The 16S rRNA gene sequence analysis revealed a clear affiliation of strain YIM 101302T to the genus Sphingobacterium. The newly isolated bacterium was found to be closely related to Sphingobacterium composti T5-12T (97.1 % 16S rRNA sequence identity) and Sphingobacterium alimentarium WCC 4521T (95.6 % 16S rRNA sequence identity) forming a distinct clade with these two species. Polar lipids of strain YIM 101302T were identified as phosphatidylethanolamine, phosphatidylmethylethanolamine, phosphatidylglycerol, phosphatidylinositol, an unidentified aminophospholipid, and three unidentified polar lipids; the predominant menaquinone as MK-7 and the major fatty as iso-C_15:0. The genomic DNA G+C content was determined to be 38.9 mol%. The DNA–DNA hybridization values between strain YIM 101302T and S. composti T5-12T, was 53.6 ± 5.8 %. These results indicates that strain YIM 101302T represents a novel species of the genus Sphingobacterium, for which the name Sphingobacterium rhinocerotis sp. nov. is proposed. The type strain is YIM 101302T (=CCTCC AB 2013218T = KCTC 42533T). Keywords Sphingobacterium rhinocerotis sp. nov. Faeces Rhinoceros unicornis