Novosphingobium aquaticum sp. nov., isolated from lake water in Suwon, Republic of Korea

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• 作者：Hina Singh ; Juan Du ; Jung-Eun Yang ; ChangShik Yin ; MooChang Kook…
• 关键词：Novosphingobium aquaticum ; Ubiquinone Q ; 10 ; Gram ; stain negative ; 16S rRNA
• 刊名：Antonie van Leeuwenhoek
• 出版年：2015
• 出版时间：October 2015
• 年：2015
• 卷：108
• 期：4
• 页码：851-858
• 全文大小：447 KB
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• 作者单位：Hina Singh (1)
  Juan Du (1)
  Jung-Eun Yang (1)
  ChangShik Yin (2)
  MooChang Kook (3)
  Tae-Hoo Yi (1)
  
  1. Department of Oriental Medicine Biotechnology, College of Life Science, Kyung Hee University Global Campus, 1732 Deokyoungdaero, Giheung-gu, Yongin-si, Gyeonggi-do, 446-701, Republic of Korea
  2. Acupunture Merdian Science Research Center, College of Korean Medicine, Kyung Hee University Global Campus, 1732 Deokyoungdaero, Giheung-gu, Yongin-si, Gyeonggi-do, 446-701, Republic of Korea
  3. Department of Marine Biotechnology, Anyang University, Incheon, 417-833, Republic of Korea
  
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Microbiology
  Medical Microbiology
  Plant Sciences
  Soil Science and Conservation
  
• 出版者：Springer Netherlands
• ISSN：1572-9699

文摘

A novel Gram-stain negative, yellow coloured, strictly aerobic, rod-shaped, non-motile bacterium designated as THW-SA1T, was isolated from lake water near Samsung apartment, Suwon, Republic of Korea. The phylogenetic analysis based on 16S rRNA gene sequences showed that strain THW-SA1T belongs to the genus Novosphingobium and is closely related to Novosphingobium taihuense (97.8 %) and Novosphingobium subterraneum (97.1 %). The DNA–DNA relatedness values between strain THW-SA1T and the most closely related type strains were found to be less than 30.0 %. The DNA G+C content was determined to be 67.5 mol%. The strain grows optimally at 25-8 °C, at pH 7.0, and in the presence of 0.5 % NaCl. The predominant isoprenoid quinone was identified as ubiquinone Q-10. The polar lipid profile comprises diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidyldimethylethanolamine, sphingoglycolipid, phosphatidylcholine, some unidentified phospholipids and some unidentified polar lipids. Fatty acids characteristic for this genus, such as C_16:1, C_14:0 2-OH, C_16:1 ω6c and/or C_16:1 ω7c (summed feature 3) and C_18:1 ω6c and/or C_18:1 ω7c (summed feature 8) were also detected. On the basis of the phenotypic and genotypic analysis, the strain THW-SA1T is considered to represent a novel species of the genus Novosphingobium, for which the name Novosphingobium aquaticum sp. nov. is proposed. The type strain is THW-SA1T (=KCTC 42608T=CCTCC AB 2015114T). Keywords Novosphingobium aquaticum Ubiquinone Q-10 Gram-stain negative 16S rRNA