Protective Roles of Selenium on Nitric Oxide and the Gene Expression of Inflammatory Cytokines Induced by Cadmium in Chicken Splenic Lymphocytes

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• 作者：Shuang Liu ; Fengping Xu ; Jing Fu ; Shu Li
• 关键词：Cadmium ; Selenium ; Inflammation ; Cytokines ; Nitric oxide ; Chicken splenic lymphocytes
• 刊名：Biological Trace Element Research
• 出版年：2015
• 出版时间：November 2015
• 年：2015
• 卷：168
• 期：1
• 页码：252-260
• 全文大小：889 KB
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• 作者单位：Shuang Liu (1)
  Fengping Xu (1)
  Jing Fu (2)
  Shu Li (1)
  
  1. College of Veterinary Medicine, Northeast Agricultural University, Harbin, 150030, People’s Republic of China
  2. College of Animal Science and Technology, Northeast Agricultural University, Harbin, 150030, People’s Republic of China
  
• 刊物主题：Biochemistry, general; Biotechnology; Nutrition; Oncology;
• 出版者：Springer US
• ISSN：1559-0720

文摘

Cadmium (Cd) is an environmental toxicant and an inflammation-related xenobiotic. Selenium (Se) is a well-known nutritional trace element and a potent chemopreventive agent. The present study aimed to investigate the effect of Se on the cytotoxicity of Cd in bird immunocytes in vitro. Chicken splenic lymphocytes exposed to CdCl₂ (10? mol/L), Na₂SeO₃ (10? mol/L), or a mixture of the two (10? mol/L Na₂SeO₃ and 10? mol/L CdCI₂) were incubated for 12, 24, 36, 48, or 60 h. Cd significantly increased (P-lt;-.05 or P-lt;-.01) the messenger RNA (mRNA) expression levels of nuclear factor kappaB (NF-κB), inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), tumor necrosis factor (TNF-α), and prostaglandin E₂ (PGE₂), and similar results were observed in the protein expression levels of NF-κB and COX-2. In addition, the nitric oxide (NO) content and the inducible iNOS activity were increased in the Cd-treated group compared to the control group. Furthermore, the protective effects of Se against Cd toxicity in chicken splenic lymphocytes were illustrated by the increase in select cytokines (NF-κB, iNOS, COX-2, TNF-α, and PGE₂), NO content and iNOS activity. The biochemical parameters exhibited sensitivity to Se and Cd, suggesting that they may act as potential biomarkers for assessing the effects of Se and Cd risk on chicken splenic lymphocytes. Keywords Cadmium Selenium Inflammation Cytokines Nitric oxide Chicken splenic lymphocytes