Enhanced viability and neural differential potential in poor post-thaw hADSCs by agarose multi-well dishes and spheroid culture

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• 作者：Xiaoling Guo ; Shanyi Li ; Qingshan Ji ; Ruiling Lian ; Jiansu Chen
• 关键词：Human adipose derived stem cells (hADSCs) ; Post ; thaw hADSCs ; Viability ; Spheroid formation ; Differentiation
• 刊名：Human Cell
• 出版年：2015
• 出版时间：October 2015
• 年：2015
• 卷：28
• 期：4
• 页码：175-189
• 全文大小：4,082 KB
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• 作者单位：Xiaoling Guo (1)
  Shanyi Li (1)
  Qingshan Ji (4)
  Ruiling Lian (3)
  Jiansu Chen (1) (2) (3)
  
  1. Key Laboratory for Regenerative Medicine, Ministry of Education, Jinan University, Guangzhou, China
  4. Department of Ophthalmology, Affiliated Anhui Provincial Hospital of Anhui Medical University, Hefei, China
  3. Department of Ophthalmology, The First Clinical Medical College of Jinan University, Jinan University, Guangzhou, 510632, China
  2. Eye Institute, Medical College of Jinan University, Jinan University, Guangzhou, China
  
• 刊物主题：Cell Biology; Embryology; Oncology; Stem Cells; Reproductive Medicine; Cell Culture;
• 出版者：Springer Japan
• ISSN：1749-0774

文摘

Human adipose-derived stem cells (hADSCs) are potential adult stem cells source for cell therapy. But hADSCs with multi-passage or cryopreservation often revealed poor growth performance. The aim of our work was to improve the activity of poor post-thaw hADSCs by simple and effective means. We describe here a simple method based on commercially available silicone micro-wells for creating hADSCs spheroids to improve viability and neural differentiation potential on poor post-thaw hADSCs. The isolated hADSCs positively expresse d CD29, CD44, CD105, and negatively expressed CD34, CD45, HLA-DR by flow cytometry. Meanwhile, they had adipogenic and osteogenic differentiation capacity. The post-thaw and post-spheroid hADSCs from poor growth status hADSCs showed a marked increase in cell proliferation by CKK-8 analysis, cell cycle analysis and Ki67/P27 quantitative polymerase chain reaction (qPCR) analysis. They also displayed an increase viability of anti-apoptosis by annexin v and propidium iodide assays and mitochondrial membrane potential assays. After 3 days of neural induction, the neural differentiation potential of post-thaw and post-spheroid hADSCs could be enhanced by qPCR analysis and western blotting analysis. These results suggested that the spheroid formation could improve the viability and neural differentiation potential of bad growth status hADSCs, which is conducive to ADSCs research and cell therapy. Keywords Human adipose derived stem cells (hADSCs) Post-thaw hADSCs Viability Spheroid formation Differentiation