Genetic diversity of Ralstonia solanacearum phylotype II sequevar 4 strains associated with Moko disease of banana (Musa spp.) in Peninsular Malaysia

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• 作者：Dzarifah Zulperi ; Kamaruzaman Sijam…
• 关键词：Moko disease ; Banana ; Ralstonia solanacearum strains ; Phylotyping ; Sequevar ; Rep ; PCR ; Genetic diversity
• 刊名：European Journal of Plant Pathology
• 出版年：2016
• 出版时间：February 2016
• 年：2016
• 卷：144
• 期：2
• 页码：257-270
• 全文大小：1,715 KB
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• 作者单位：Dzarifah Zulperi (1)
  Kamaruzaman Sijam (1)
  Zainal Abidin Mior Ahmad (1)
  Yahya Awang (2)
  Siti Izera Ismail (1)
  Norhayu Asib (1)
  Erneeza Mohd Hata (1)
  
  1. Department of Plant Protection, Faculty of Agriculture, Universiti Putra Malaysia, Serdang, Selangor, Malaysia
  2. Department of Crop Science, Faculty of Agriculture, Universiti Putra Malaysia, Serdang, Selangor, Malaysia
  
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Plant Pathology
  Plant Sciences
  Ecology
  
• 出版者：Springer Netherlands
• ISSN：1573-8469

文摘

Moko disease caused by Ralstonia solanacearum (R. solanacearum) is a major disease affecting banana (Musa spp.) production. Although local reports suggested that this disease is widespread in Malaysia, molecular characterization of R. solanacearum strains associated with Moko disease in this country has not yet been done. During March 2011 to June 2012, 170 banana plants associated with Moko disease and the adjacent soil samples were collected in 12 different locations of five outbreak states in Peninsular Malaysia comprising Kedah, Selangor, Pahang, Negeri Sembilan and Johor, with disease incidence exceeding 80 % in some severely affected plantations. A total of 197 strains were identified as R. solanacearum-like colonies since they produced fluidal colonies that were white to pink coloration after incubation at 24 to 48 h at 29 掳C on Kelman鈥檚 TZC agar medium, appeared as Gram-negative rods, and positive for potassium hydroxide (KOH), Kovacs oxidase, catalase and lipase activity on Tween 80 solution tests. Biovar tests disclosed that only 30 strains displayed characteristics of biovar 1 R. solanacearum associated with Moko disease, which was negative for utilization of disaccharides and hexose alcohols. Pathogenicity assay showed that these 30 strains were virulence towards Musa paradisiaca cv. Nipah explants with diverse degrees of virulence. Phylotype-specific multiplex PCR (Pmx-PCR) revealed all strains belonged to phylotype II displaying a 372 bp amplicon. Phylogenetic analyses of endoglucanase (egl) sequences clustered all 30 strains into phylotype II/4, together with the reference sequences strains from Peru (UW129, UW162 and UW163) and Colombia (UW070). Pooled rep-PCR fingerprinting method defined two major groups; cluster 1 (sub-group A and B) and cluster 2 (sub-group C), with 35 % average similarity coefficient within these two clusters. The sub-groups in cluster 1 were represented by strains from Kedah, Selangor, Negeri Sembilan and Johor; while cluster 2 sub-group was represented exclusively by strains of Pahang. To our knowledge, this is the first description of R. solanacearum phylotype II/4 in Malaysia and the Asian region. Our findings may expand constructive documentation and describe a better understanding on diversity of Malaysian R. solanacearum Moko-causing strains populations, thus will be useful for designing disease control strategies. Keywords Moko disease Banana Ralstonia solanacearum strains Phylotyping Sequevar Rep-PCR Genetic diversity