Unraveling the in vitro and in vivo metabolism of diacetoxyscirpenol in various animal species and human using ultrahigh-performance liquid chromatography-quadrupole/time-of-flight hybrid mass spectrometry

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• 作者：Shupeng Yang ; Marthe De Boevre ; Huiyan Zhang…
• 关键词：Diacetoxyscirpenol ; Metabolism ; Phase I and II metabolites ; Animal ; Human ; UHPLC ; Q/TOF
• 刊名：Analytical and Bioanalytical Chemistry
• 出版年：2015
• 出版时间：November 2015
• 年：2015
• 卷：407
• 期：28
• 页码：8571-8583
• 全文大小：1,822 KB
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• 作者单位：Shupeng Yang (1)
  Marthe De Boevre (2)
  Huiyan Zhang (1)
  Karl De Ruyck (2)
  Feifei Sun (1)
  Zhanhui Wang (1)
  Xingyuan Cao (1)
  Jianzhong Shen (1)
  Sarah De Saeger (2)
  Suxia Zhang (1)
  
  1. College of Veterinary Medicine, China Agricultural University, Beijing Laboratory for Food Quality and Safety, Beijing Key Laboratory of Detection Technology for Animal-Derived Food Safety, National Reference Laboratory of Veterinary Drug Residues, Beijing, 100193, China
  2. Laboratory of Food Analysis, Faculty of Pharmaceutical Sciences, Ghent University, Ottergemsesteenweg 460, 9000, Ghent, Belgium
  
• 刊物类别：Chemistry and Materials Science
• 刊物主题：Chemistry
  Analytical Chemistry
  Food Science
  Inorganic Chemistry
  Physical Chemistry
  Monitoring, Environmental Analysis and Environmental Ecotoxicology
  
• 出版者：Springer Berlin / Heidelberg
• ISSN：1618-2650

文摘

Diacetoxyscirpenol (DAS), a Fusarium mycotoxin belonging to the trichothecene type A mycotoxins, is able to contaminate food and feed worldwide. Only limited information is available regarding the metabolism of DAS. The present study used ultrahigh-performance liquid chromatography-quadrupole/time-of-flight hybrid mass spectrometry (UHPLC-Q/TOF) to investigate the in vitro phase I and II metabolism of DAS by rat, chicken, swine, goat, cow, and human liver microsomes. An extensive metabolization profile of DAS has been observed. A total of seven phase I and three phase II metabolites of DAS were detected. Among the identified molecules, four phase I metabolites (8β-hydroxy-DAS, neosolaniol, 7-hydroxy-DAS, and its epimer) and two phase II metabolites (4-deacetyl-DAS-3-glucuronic acid and 4-deacetyl-DAS-4-glucuronic acid) were identified for the first time. These results indicate that the major metabolic pathways of DAS in vitro were hydrolyzation (M1–M3), hydroxylation (M4–M7), and conjugation (M8–M10). Qualitative differences in phase I and II metabolic profiles of DAS between the five animal species and human were observed. 4-Deacetyl-DAS was the primary metabolite from liver microsomes of all species, especially human. The in vivo metabolism of DAS in rats and chickens after oral administration of DAS was also investigated and compared. The major metabolites for rats and chickens were 4-deacetyl-DAS and 7-hydroxy-DAS. These results will help to gain a more detailed insight into the metabolism and toxicity of DAS among different animal species and human.