Dietary supplementation with l-glutamate and l-aspartate alleviates oxidative stress in weaned piglets challenged with hydrogen peroxide

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• 作者：Jielin Duan ; Jie Yin ; Wenkai Ren ; Ting Liu ; Zhijie Cui ; Xingguo Huang ; Li Wu…
• 关键词：l ; Glutamate ; l ; Aspartate ; Oxidative stress ; Hydrogen peroxide ; Performance ; Pigs
• 刊名：Amino Acids
• 出版年：2016
• 出版时间：January 2016
• 年：2016
• 卷：48
• 期：1
• 页码：53-64
• 全文大小：614 KB
• 参考文献：Assaad H, Zhou L, Carroll RJ et al (2014) Rapid publication-ready MS-Word tables for one-way ANOVA. SpringerPlus 3:474PubMed PubMedCentral CrossRef
  Batterham ES, Andersen LM, Baigent DR et al (1990) Utilization of ileal digestible amino acids by pigs: lysine. Br J Nutr 64:679–690PubMed CrossRef
  Blachier F, Boutry C, Bos C et al (2009) Metabolism and functions of L-glutamate in the epithelial cells of the small and large intestines. Am J Clin Nutr 90:814S–821SPubMed CrossRef
  Boutry C, Matsumoto H, Bos C et al (2012) Decreased glutamate, glutamine and citrulline concentrations in plasma and muscle in endotoxemia cannot be reversed by glutamate or glutamine supplementation: a primary intestinal defect? Amino Acids 43:1485–1498PubMed CrossRef
  Brosnan JT, Brosnan ME (2013) Glutamate: a truly functional amino acid. Amino Acids 45:413–418PubMed CrossRef
  Chen G, Zhang J, Zhang YZ et al (2014) Oral MSG administration alters hepatic expression of genes for lipid and nitrogen metabolism in suckling piglets. Amino Acids 46:245–250PubMed CrossRef
  Dai ZL, Li XL, Xi PB et al (2012) Regulatory role for l -arginine in the utilization of amino acids by pig small-intestinal bacteria. Amino Acids 43:233–244PubMed CrossRef
  Dai ZL, Li XL, Xi PB et al (2013) l -Glutamine regulates amino acid utilization by intestinal bacteria. Amino Acids 45:501–512PubMed CrossRef
  Dai ZL, Wu ZL, Jia SC et al (2014) Analysis of amino acid composition in proteins of animal tissues and foods as pre-column o-phthaldialdehyde derivatives by HPLC with fluorescence detection. J Chromatogr B 964:116–127CrossRef
  Dai ZL, Wu ZL, Hang SQ et al (2015) Amino acid metabolism in intestinal bacteria and its potential implications for mammalian reproduction. Mol Hum Reprod 21:389–409PubMed CrossRef
  Duan J, Yin J, Wu M et al (2014) Dietary glutamate supplementation ameliorates mycotoxin-induced abnormalities in the intestinal structure and expression of amino acid transporters in young pigs. PLoS One 9:e112357PubMed PubMedCentral CrossRef
  Duan JL, Yin J, Ren WK et al (2015) Pyrrolidine dithiocarbamate restores gastric damages and suppressive autophagy induced by hydrogen peroxide. Free Radic Res 49:210–218PubMed CrossRef
  El Assar M, Angulo J, Rodriguez-Manas L (2013) Oxidative stress and vascular inflammation in aging. Free Radic Biol Med 65:380–401PubMed CrossRef
  Feng ZM, Zhou XL, Wu F et al (2014) Both dietary supplementation with monosodium l -glutamate and fat modify circulating and tissue amino acid pools in growing pigs, but with little lnteractive effect. PLoS One 9:e84533PubMed PubMedCentral CrossRef
  Feng Z, Li T, Wu C et al (2015) Monosodium l -glutamate and dietary fat exert opposite effects on the proximal and distal intestinal health in growing pigs. Appl Physiol Nutr Metab 40:353–363PubMed CrossRef
  Fukui A, Naito Y, Handa O et al (2012) Acetyl salicylic acid induces damage to intestinal epithelial cells by oxidation-related modifications of ZO-1. Am J Physiol Gastrointest Liver Physiol 303:G927–G936PubMed CrossRef
  Haynes TE, Li P, Li XL et al (2009) l -Glutamine or l -alanyl-l -glutamine prevents oxidant- or endotoxin-induced death of neonatal enterocytes. Amino Acids 37:131–142PubMed CrossRef
  Hou YQ, Yin YL, Wu G (2015) Dietary essentiality of “nutritionally nonessential amino acids” for animals and humans. Exp Biol Med. doi:10.​1177/​1535370215587913​
  Hu SD, Li XL, Rezaei R et al (2015) Safety of long-term dietary supplementation with l -arginine in pigs. Amino Acids 47:925–936PubMed CrossRef
  Ito T, Schaffer SW, Azuma J (2012) The potential usefulness of taurine on diabetes mellitus and its complications. Amino Acids 42:1529–1539PubMed PubMedCentral CrossRef
  Jin Y, Wang L, Qu S et al (2015) STAMP2 increases oxidative stress and is critical for prostate cancer. EMBO Mol Med 7:315–331PubMed PubMedCentral CrossRef
  Kang P, Liu Y, Zhu H et al (2015) The effect of aspartate on the energy metabolism in the liver of weanling pigs challenged with lipopolysaccharide. Eur J Nutr 54:581–588PubMed CrossRef
  Kim SW, Wu G (2004) Dietary arginine supplementation enhances the growth of milk-fed young pigs. J Nutr 134:625–630PubMed
  Lackeyram D, Mine Y, Widowski T et al (2012) The in vivo infusion of hydrogen peroxide induces oxidative stress and differentially affects the activities of small intestinal carbohydrate digestive enzymes in the neonatal pig. J Anim Sci 90(Suppl 4):418–420PubMed CrossRef
  Li P, Yin YL, Li DF et al (2007) Amino acids and immune function. Br J Nutr 98:237–252PubMed CrossRef
  Mandalari G, Bisignano C, Genovese T et al (2011) Natural almond skin reduced oxidative stress and inflammation in an experimental model of inflammatory bowel disease. Int Immunopharmacol 11:915–924PubMed CrossRef
  Minamoto Y, Otoni CC, Steelman SM et al (2015) Alteration of the fecal microbiota and serum metabolite profiles in dogs with idiopathic inflammatory bowel disease. Gut Microbes 6:33–47PubMed PubMedCentral CrossRef
  Oba M, Baldwin RL, Bequette BJ (2004) Oxidation of glucose, glutamate, and glutamine by isolated ovine enterocytes in vitro is decreased by the presence of other metabolic fuels. J Anim Sci 82:479–486PubMed
  Pi D, Liu Y, Shi H et al (2014) Dietary supplementation of aspartate enhances intestinal integrity and energy status in weanling piglets after lipopolysaccharide challenge. J Nutr Biochem 25:456–462PubMed CrossRef
  Ren W, Luo W, Wu M et al (2013a) Dietary l -glutamine supplementation improves pregnancy outcome in mice infected with type-2 porcine circovirus. Amino Acids 45:479–488PubMed CrossRef
  Ren W, Zou L, Li N et al (2013b) Dietary arginine supplementation enhances immune responses to inactivated Pasteurella multocida vaccination in mice. Br J Nutr 109:867–872PubMed CrossRef
  Ren W, Liu S, Chen S et al (2013c) Dietary l -glutamine supplementation increases Pasteurella multocida burden and the expression of its major virulence factors in mice. Amino Acids 45:947–955PubMed CrossRef
  Ren W, Chen S, Yin J et al (2014a) Dietary arginine supplementation of mice alters the microbial population and activates intestinal innate immunity. J Nutr 144:988–995PubMed CrossRef
  Ren W, Duan J, Yin J et al (2014b) Dietary l -glutamine supplementation modulates microbial community and activates innate immunity in the mouse intestine. Amino Acids 46:2403–2413PubMed CrossRef
  Ren WK, Yin J, Wu MM et al (2014d) Serum amino acids profile and the beneficial effects of l -arginine or l -glutamine supplementation in dextran sulfate sodium solitis. Plos One 9:e88335PubMed PubMedCentral CrossRef
  Rezaei R, Wang W, Wu Z et al (2013a) Biochemical and physiological bases for utilization of dietary amino acids by young Pigs. J Anim Sci Biotechnol 4:7PubMed PubMedCentral CrossRef
  Rezaei R, Knabe DA, Tekwe CD et al (2013b) Dietary supplementation with monosodium glutamate is safe and improves growth performance in postweaning pigs. Amino Acids 44:911–923PubMed CrossRef
  Russell RR, Taegtmeyer H (1991) Changes in citric acid cycle flux and anaplerosis antedate the functional decline in isolated rat hearts utilizing acetoacetate. J Clin Invest 87:384–390PubMed PubMedCentral CrossRef
  San Gabriel A, Uneyama H (2013) Amino acid sensing in the gastrointestinal tract. Amino Acids 45:451–461PubMed CrossRef
  Singsen EP, Nagel J, Patrick SG et al (1965) The effect of a lysine deficiency on growth characteristics, age at sexual maturity, and reproductive performance of meat-type pullets. Poult Sci 44:1467–1473PubMed CrossRef
  Sivakumar R, Babu PV, Shyamaladevi CS (2011) Aspartate and glutamate prevents isoproterenol-induced cardiac toxicity by alleviating oxidative stress in rats. Exp Toxicol Pathol 63:137–142PubMed CrossRef
  Stoll B, Burrin DG, Henry J et al (1999) Substrate oxidation by the portal drained viscera of fed piglets. Am J Physiol 277:E168–E175PubMed
  Sun YL, Wu ZL, Li W et al (2015) Dietary l -leucine supplementation enhances intestinal development in suckling piglets. Amino Acids 47:1517–1525PubMed CrossRef
  Togliatto G, Trombetta A, Dentelli P et al (2015) Unacylated ghrelin (UnAG) induces oxidative stress resistance in a glucose intolerance mouse model and peripheral artery disease by restoring endothelial cell miR-126 expression. Diabetes 64:1370–1382PubMed CrossRef
  Wang JJ, Chen LX, Li P et al (2008a) Gene expression is altered in piglet small intestine by weaning and dietary glutamine supplementation. J Nutr 138:1025–1032PubMed CrossRef
  Wang JJ, Chen LX, Li DF et al (2008b) Intrauterine growth restriction affects the proteomes of the small intestine, liver and skeletal muscle in newborn pigs. J Nutr 138:60–66PubMed CrossRef
  Wang JJ, Wu ZL, Li DF et al (2012) Nutrition, epigenetics, and metabolic syndrome. Antioxid Redox Signal 17:282–301PubMed PubMedCentral CrossRef
  Wang WW, Wu ZL, Dai ZL et al (2013) Glycine metabolism in animals and humans: implications for nutrition and health. Amino Acids 45:463–477PubMed CrossRef
  Wang WW, Dai ZL, Wu ZL et al (2014a) Glycine is a nutritionally essential amino acid for maximal growth of milk-fed young pigs. Amino Acids 46:2037–2045PubMed CrossRef
  Wang WW, Wu ZL, Lin G et al (2014b) Glycine stimulates protein synthesis and inhibits oxidative stress in pig small-intestinal epithelial cells. J Nutr 144:1540–1548PubMed CrossRef
  Wang H, Zhang C, Wu G et al (2015a) Glutamine enhances tight-junction protein expression and modulates CRF signaling in the jejunum of weanling piglets. J Nutr 145:25–31PubMed CrossRef
  Wang H, Ji Y, Wu G et al (2015b) L-Tryptophan activates mammalian target of rapamycin and enhances expression of tight junction proteins in intestinal porcine epithelial cells. J Nutr 145:1156–1162PubMed CrossRef
  Wei JW, Carroll RJ, Harden KK et al (2012) Comparisons of treatment means when factors do not interact in two-factorial studies. Amino Acids 42:2031–2035PubMed PubMedCentral CrossRef
  Wu G (1998) Intestinal mucosal amino acid catabolism. J Nutr 8:1249–1252
  Wu G (2009) Amino acids: metabolism, functions, and nutrition. Amino Acids 37:1–17PubMed CrossRef
  Wu G (2010) Functional amino acids in growth, reproduction, and health. Adv Nutr 1:31–37PubMed PubMedCentral CrossRef
  Wu G (2013a) Functional amino acids in nutrition and health. Amino Acids 45:407–411PubMed CrossRef
  Wu G (2013b) Amino acids: biochemistry and nutrition. CRC Press, Boca Raton, FloridaCrossRef
  Wu G (2014) Dietary requirements of synthesizable amino acids by animals: a paradigm shift in protein nutrition. J Anim Sci Biotechnol 5:34PubMed PubMedCentral CrossRef
  Wu G, Fang YZ, Yang S et al (2004) Glutathione metabolism and its implications for health. J Nutr 134:489–492PubMed
  Wu X, Yin YL, Li TJ, Wang L, Ruan Z, Liu ZQ, Hou YQ (2010) Dietary protein, energy and arginine affect LAT1 expression in forebrain white matter differently. Animal 4:1518–1521PubMed CrossRef
  Wu X, Zhang Y, Liu Z et al (2012) Effects of oral supplementation with glutamate or combination of glutamate and N-carbamylglutamate on intestinal mucosa morphology and epithelium cell proliferation in weanling piglets. J Anim Sci 90(Suppl 4):337–339PubMed CrossRef
  Wu X, Xie C, Yin YL, Li FN, Li TJ, Huang RL, Deng ZY (2013a) Effect of l -arginine on HSP70 expression in liver in weanling piglets. BMC Veterinary Research 9:63PubMed PubMedCentral CrossRef
  Wu X, Shu XG, Xie CY, Li TJ, Hu J, Yin YL, Deng ZY (2013b) The acute and chronic effects of monosodium l -Glutamate on Serum Iron and Total Iron-Binding Capacity in the Jugular Artery and Vein of Pigs. Biol Trace Elem Res 153:191–195CrossRef
  Wu G, Wu ZL, Dai ZL et al (2013c) Dietary requirements of “nutritionally non-essential amino acids” by animals and humans. Amino Acids 44:1107–1113PubMed CrossRef
  Wu M, Xiao H, Ren W et al (2014a) Therapeutic effects of glutamic acid in piglets challenged with deoxynivalenol. PLoS One 9:e100591PubMed PubMedCentral CrossRef
  Wu M, Xiao H, Ren W et al (2014b) An NMR-based metabolomic approach to investigate the effects of supplementation with glutamic acid in piglets challenged with deoxynivalenol. PLoS One 9:e113687PubMed PubMedCentral CrossRef
  Wu G, Bazer FW, Dai ZL et al (2014c) Amino acid nutrition in animals: protein synthesis and beyond. Annu Rev Anim Biosci 2:387–417PubMed CrossRef
  Wu G, Fanzo J, Miller DD et al (2014d) Production and supply of high-quality food protein for human consumption: sustainability, challenges and innovations. Ann NY Acad Sci 1321:1–19PubMed CrossRef
  Wu G, Bazer FW, Cross HR (2014e) Land-based production of animal protein: impacts, efficiency, and sustainability. Ann NY Acad Sci 1328:18–28PubMed CrossRef
  Wu X, Xie CY, Zhang YZ, Fan ZY, Yin YL, Blachier F (2015) Glutamate–glutamine cycle and exchange in the placenta–fetus unit during late pregnancy. Amino Acids 47:45–53
  Wu L, Liao P, He L et al (2015b) Dietary l -arginine supplementation protects weanling pigs from deoxynivalenol-induced toxicity. Toxins (Basel) 7:1341–1354CrossRef
  Wu X, Xie C, Zhang Y et al (2015c) Glutamate-glutamine cycle and exchange in the placenta-fetus unit during late pregnancy. Amino Acids 47:45–53PubMed CrossRef
  Yin J, Ren W, Liu G et al (2013a) Birth oxidative stress and the development of an antioxidant system in newborn piglets. Free Radical Res 47:1027–1035CrossRef
  Yin J, Ren WK, Wu XS et al (2013b) Oxidative stress-mediated signaling pathways: a review. J Food Agric Environ 11:132–139
  Yin J, Ren WK, Duan JL et al (2014a) Dietary arginine supplementation enhances intestinal expression of SLC7A7 and SLC7A1 and ameliorates growth depression in mycotoxin-challenged pigs. Amino Acids 46:883–892PubMed CrossRef
  Yin J, Wu MM, Xiao H et al (2014b) Development of an antioxidant system after early weaning in piglets. J Anim Sci 92:612–619PubMed CrossRef
  Yin J, Duan JL, Cui ZJ et al (2015a) Hydrogen peroxide-induced oxidative stress activates NF-kappa B and Nrf2/Keap1 signals and triggers autophagy in piglets. Rsc Advances 5:15479–15486CrossRef
  Yin J, Liu M, Ren W et al (2015b) Effects of dietary supplementation with glutamate and aspartate on diquat-induced oxidative stress in piglets. PLoS One 10:e0122893PubMed PubMedCentral CrossRef
  Yin J, Ren W, Yang G et al (2015c) l -Cysteine metabolism and its nutritional implications. Mol Nutr Food Res. doi:10.​1002/​mnfr.​201500031
  Young D, Fan MZ, Mine Y (2010) Egg yolk peptides up-regulate glutathione synthesis and antioxidant enzyme activities in a porcine model of intestinal oxidative stress. J Agric Food Chem 58:7624–7633PubMed CrossRef
  Zhang J, Yin YL, Shu XG et al (2013) Oral administration of MSG increases expression of glutamate receptors and transporters in the gastrointestinal tract of young piglets. Amino Acids 45:1169–1177PubMed CrossRef
  Zhu LH, Xu JX, Zhu SW et al (2014) Gene expression profiling analysis reveals weaning-induced cell cycle arrest and apoptosis in the small intestine of pigs. J Anim Sci 92:996–1006PubMed CrossRef
  
• 作者单位：Jielin Duan (1)
  Jie Yin (1)
  Wenkai Ren (1)
  Ting Liu (3)
  Zhijie Cui (1)
  Xingguo Huang (3)
  Li Wu (1)
  Sung Woo Kim (4)
  Gang Liu (1)
  Xi Wu (1)
  Guoyao Wu (5)
  Tiejun Li (1) (6) (7) (8)
  Yulong Yin (1) (2)
  
  1. Scientific Observing and Experimental Station of Animal Nutrition and Feed Science in South-Central, Ministry of Agriculture, Hunan Provincial Engineering Research Center of Healthy Livestock, Key Laboratory of Agro-ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha, 410125, Hunan, China
  3. College of Animal Science and Technology, Hunan Agricultural University, Changsha, 410128, Hunan, China
  4. Department of Animal Science, North Carolina State University, Raleigh, 27695, USA
  5. Department of Animal Science, Texas A&M University, College Station, TX, 77843, USA
  6. Changsha Lvye Biotechnology Limited Company Academician Expert Workstation, Changsha, 410128, Hunan, China
  7. Guangdong Wangda Group Academician Workstation for Clean Feed Technology Research and Development in Swine, Guangzhou, 510663, Guangdong, China
  8. Guangdong Hinapharm Group Academician Workstation for Biological Feed and Feed Additives and Animal Intestinal Health, Guangzhou, 511400, Guangdong, China
  2. School of Biology, Hunan Normal Univesity, Changsha, 410018, Hunan, China
  
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Biochemistry
  Analytical Chemistry
  Biochemical Engineering
  Life Sciences
  Proteomics
  Neurobiology
  
• 出版者：Springer Wien
• ISSN：1438-2199

文摘

This study was to evaluate the protective roles of l-glutamate (Glu) and l-aspartate (Asp) in weaned piglets challenged with H₂O₂. Forty weaned piglets were assigned randomly into one of five groups (8 piglets/group): (1) control group (NC) in which pigs were fed a corn- and soybean meal-based diet and received intraperitoneal administration of saline; (2) H₂O₂ group (PC) in which pigs were fed the basal diet and received intraperitoneal administration of 10 % H₂O₂ (1 ml/kg body weight once on days 8 and repeated on day 11); (3) PC + Glu group (PG) in which pigs were fed the basal diet supplemented with 2.0 % Glu before intraperitoneal administration of 10 % H₂O₂; (4) PC + Asp group (PA) in which pigs were fed the basal diet supplemented with 1.0 % Asp before intraperitoneal administration of 10 % H₂O₂; (5) PC + Glu + Asp group (PGA) in which pigs were fed the basal diet supplemented with 2.0 % Glu plus 1.0 % Asp before intraperitoneal administration of 10 % H₂O₂. Measured parameters included daily feed intake (DFI), average daily gain (ADG), feed conversion rate (FCR), and serum anti-oxidative enzyme activities (catalase, superoxide dismutase, glutathione peroxidase-1), serum malondialdehyde and H₂O₂ concentrations, serum amino acid (AA) profiles, and intestinal expression of AA transporters. Dietary supplementation with Glu, Asp or their combination attenuated the decreases in DFI, ADG and feed efficiency, the increase in oxidative stress, the alterations of serum AA concentrations, and the changed expression of intestinal AA transporters in H₂O₂-challenged piglets. Thus, dietary supplementation with Glu or Asp alleviates growth suppression and oxidative stress, while restoring serum the amino acid pool in H₂O₂-challenged piglets. Keywords l-Glutamate l-Aspartate Oxidative stress Hydrogen peroxide Performance Pigs