Protective Effects of Coenzyme Q10 Against Hydrogen Peroxide-Induced Oxidative Stress in PC12 Cell: The Role of Nrf2 and Antioxidant Enzymes

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• 作者：Li Li ; Jikun Du ; Yaru Lian ; Yun Zhang ; Xingren Li…
• 关键词：Coenzyme Q10 ; Antioxidant enzyme ; Nrf2 ; Neurodegeneration
• 刊名：Cellular and Molecular Neurobiology
• 出版年：2016
• 出版时间：January 2016
• 年：2016
• 卷：36
• 期：1
• 页码：103-111
• 全文大小：642 KB
• 参考文献：Beal MF (2003) Bioenergetic approaches for neuroprotection in Parkinson’s disease. Ann Neurol 53(3):39–47CrossRef
  Bhat V, Weiner WJ (2005) Parkinson’s disease. diagnosis and the initiation of therapy. Minerva Med 96:145–154PubMed
  Dinis TC, Maderia VM, Almeida L (1994) Action of phenolic derivatives (acetaminophen, salicylate, and 5-aminosalicylate) as inhibitors of membrane lipid peroxidation and as peroxyl radical scavengers. Arch Biochem Biophys 315:161–169PubMed CrossRef
  Feigin A, Kieburtz K, Como P, Hickey C, Claude K, Abwender D, Zimmerman C, Steinberg K, Shoulson I (1996) Assessment of coenzyme Q10 tolerability in Huntington’s disease. Mov Disord 11:321–323PubMed CrossRef
  Ferrante RJ, Andreassen OA, Dedeoglu A, Ferrante KL, Jenkins BG, Hersch SM, Beal MF (2002) Therapeutic effects of coenzyme Q10 and remacemide in transgenic mouse models of Huntington’s disease. J Neurosci 22(5):1592–1599PubMed
  Ferrante KL, Shefner J, Zhang H, Betensky R, O’Brien M, Yu H, Fantasia M, Taft J, Beal MF, Traynor B, Newhall K, Donofrio P, Caress J, Ashburn C, Freiberg B, O’Neill C, Paladenech C, Walker T, Pestronk A, Abrams B, Florence J, Renna R, Schierbecker J, Malkus B, Cudkowicz M (2005) Tolerance of high-dose (3,000 mg/day) coenzyme Q10 in ALS. Neurology 65(11):1834–1836PubMed CrossRef
  Gazdík F, Piják MR, Borová A, Gazdíková K (2003) Biological properties of coenzyme Q10 and its effects on immunity. Cas Lek Cesk 142(7):390–393PubMed
  Glass CK, Saijo K, Winner B, Marchetto MC, Gage FH (2010) Mechanisms underlying inflammation in neurodegeneration. Cell 140(6):918–934PubMed PubMedCentral CrossRef
  Guroff G (1985) PC12 cells as a model of neuronal differentiation. Bottenstein JE Cell culture in neurosciences. Plemun Press, New York, pp 245–272CrossRef
  Itoh K, Ishii T, Wakabayashi N, Yamamoto M (1999) Regulatory mechanisms of cellular response to oxidative stress. Free Radical Res 31(4):319–324CrossRef
  Itoh K, Wakabayashi N, Katoh Y, Ishii T, O’Connor T, Yamamoto M (2003) Keap1 regulates both cytoplasmic–nuclearshuttling and degradation of Nrf2 in response to electrophiles. Genes Cells 8(4):379–391PubMed CrossRef
  Johnson JA, Johnson DA, Kraft AD, Calkins MJ, Jakel RJ, Vargas MR, Chen PC (2008) The Nrf2–ARE pathway: an indicator and modulator of oxidative stress in neurodegeneration. Ann N Y Acad Sci 1147:61–69PubMed PubMedCentral CrossRef
  Kensler TW, Wakabayashi N, Biswal S (2007) Cell survival responses to environmental stresses via the Keap1–Nrf2–-ARE pathway. Annu Rev Pharmacol Toxicol 47:89–116PubMed CrossRef
  Khandhar SM, Marks WJ (2007) Epidemiology of Parkinson’s disease. Dis Mon 53(4):200–205PubMed CrossRef
  LaFerla FM, Green KN, Oddo S (2007) Intracellular amyloid-beta in Alzheimer’s disease. Nat Rev Neurosci 8:499–509PubMed CrossRef
  Lee JM, Shih AY, Murphy TH, Johnson JA (2003) NF–E2–related factor–2 mediates neuroprotection against mitochondrial complex I inhibitors and increased concentrations of intracellular calcium in primary cortical neurons. J Biol Chem 278:37948–37956PubMed CrossRef
  Li L, Du JK, Zou LY, Wu T, Lee YW, Kim YH (2013) Decursin isolated from Angelica gigas Nakai rescues PC12 Cells from amyloid β–protein–induced neurotoxicity through Nrf2-Mediated upregulation of heme oxygenase-1: potential roles of MAPK. Evid-Based Complement Alternate Med. doi:10.​1155/​2013/​467245
  Matthews RT, Yang L, Browne S, Baik M, Beal MF (1998) Coenzyme Q10 administration increases brain mitochondrial concentrations and exerts neuroprotective effects. Proc Natl Acad Sci USA 95(15):8892–8897PubMed PubMedCentral CrossRef
  Mukai FH, Goldstein BD (1976) Mutagenicity of malondialdehyde, a decomposition product of peroxidised polyunsaturated fatty acids. Science 191:868–869PubMed CrossRef
  Nguyen T, Nioi P, Pickett CB (2009) The Nrf2-antioxidant response element signaling pathway and its activation by oxidative stress. J Biol Chem 284:13291–13295PubMed PubMedCentral CrossRef
  Pi J, Zhang Q, Woods CG, Wong V, Collins S, Andersen ME (2008) Activation of Nrf2-mediated oxidative stress response in macrophages by hypochlorous acid. Toxicol Appl Pharmacol 226:236–243PubMed CrossRef
  Pratico D, Clark CM, Liun F, Rokach J, Lee VY, Trojanowski JQ (2002) Increase of brain oxidative stress in mild cognitive impairment: a possible predictor of Alzheimer disease. Arch Neurol 59(6):972–976PubMed CrossRef
  Reeve K, Krishnan KJ, Turnbull D (2008) Mitochondrial DNA mutations in disease, aging, and neurodegeneration. Ann N Y Acad Sci 1147:21–29PubMed CrossRef
  Saeidnia S, Abdollahi M (2013) Toxicological and pharmacological concerns on oxidative stress and related diseases. Toxicol Appl Pharmacol 273:442–455PubMed CrossRef
  Sen CK, Packer L (1996) Antioxidant and redox regulation of gene transcription. FASEB J 10:709–720PubMed
  Shih AY, Imbeault S, Barakauskas V, Erb H, Jiang L, Li P, Murphy TH (2005) Induction of the Nrf2–driven antioxidant response confers neuroprotection during mitochondrial stress in vivo. J Biol Chem 280(24):22925–22936PubMed CrossRef
  Shults CW, Haas R (2005) Clinical trials of coenzyme Q10 in neurological disorders. BioFactors 25(1–4):117–126PubMed CrossRef
  Shults CW, Oakes D, Kieburtz K, Beal MF, Haas R, Plumb S, Juncos JL, Nutt J, Shoulson I, Carter J, Kompoliti K, Perlmutter JS, Reich S, Stern M, Watts RL, Kurlan R, Molho E, Harrison M, Lew M (2002) Effects of coenzyme Q10 in early Parkinson disease: evidence of slowing of the functional decline. Arch Neurol 59(10):1541–1550PubMed CrossRef
  
• 作者单位：Li Li (1)
  Jikun Du (2)
  Yaru Lian (3)
  Yun Zhang (3)
  Xingren Li (3)
  Ying Liu (3)
  Liyi Zou (3)
  Tie Wu (3)
  
  1. Dongguan Scientific Research Center, Guangdong Medical University, Dongguan, 523-808, China
  2. Department of Clinical Laboratory, Shenzhen Shajing Affiliated Hospital of Guangzhou Medical University, Shenzhen, 518-104, China
  3. Department of Pharmacology, Guangdong Medical University, Dongguan, 523-808, China
  
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Biomedicine
  Neurosciences
  Animal Anatomy, Morphology and Histology
  
• 出版者：Springer Netherlands
• ISSN：1573-6830

文摘

Oxidative stress is a major component of harmful cascades activated in neurodegenerative disorders. Coenzyme Q10 (CoQ10), an essential component in the mitochondrial respiratory chain, has recently gained attention for its potential role in the treatment of neurodegenerative disease. Here, we investigated the possible protective effects of CoQ10 on H₂O₂-induced neurotoxicity in PC12 cells and the underlying mechanism. CoQ10 showed high free radical-scavenging activity as measured by a DPPH and TEAC. Pre-treatment of cells with CoQ10 diminished intracellular generation of ROS in response to H₂O₂. H₂O₂ decreased viability of PC12 cells which was reversed by pretreatment with CoQ10 according to MTT assay. H₂O₂-induced lipid peroxidation was attenuated by CoQ10 as shown by inhibition of MDA formation. Furthermore, pre-incubation of the cells with CoQ10 also restored the activity of cellular antioxidant enzymes which had been altered by H₂O₂. Moreover, CoQ10 induced Nrf2 nuclear translocation, the upstream of antioxidant enzymes. These findings suggest CoQ10 augments cellular antioxidant defense capacity through both intrinsic free radical-scavenging activity and activation of Nrf2 and subsequently antioxidant enzymes induction, thereby protecting the PC12 cells from H₂O₂-induced oxidative cytotoxicity. Keywords Coenzyme Q10 Antioxidant enzyme Nrf2 Neurodegeneration