Characterization of a protocatechuate catabolic gene cluster in Rhodococcus ruber OA1 involved in naphthalene degradation

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• 作者：Chao Li ; Chunyang Zhang ; Guanling Song ; Hong Liu ; Guihua Sheng…
• 关键词：Biodegradation ; Naphthalene ; Rhodococcus ruber ; Gene cluster ; Protocatechuate 3 ; 4 ; dioxygenase
• 刊名：Annals of Microbiology
• 出版年：2016
• 出版时间：March 2016
• 年：2016
• 卷：66
• 期：1
• 页码：469-478
• 全文大小：887 KB
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• 作者单位：Chao Li (1)
  Chunyang Zhang (1)
  Guanling Song (2)
  Hong Liu (1)
  Guihua Sheng (1)
  Zhongfeng Ding (1)
  Zhenglong Wang (1)
  Ying Sun (1)
  Yue Xu (1)
  Jing Chen (1)
  
  1. School of Life Science, Shandong University of Technology, NO.12, Zhangzhou Road, Zhangdian district, 255049, Zibo, China
  2. School of Medicine, Shihezi University, 832002, Shihezi, China
  
• 刊物主题：Microbiology; Microbial Genetics and Genomics; Microbial Ecology; Fungus Genetics; Medical Microbiology; Applied Microbiology;
• 出版者：Springer Berlin Heidelberg
• ISSN：1869-2044

文摘

In this study, we investigated a protocatechuate catabolic gene cluster involved in naphthalene degradation in Rhodococcus ruber OA1. Rhodococcus ruber OA1 was isolated from the pharmaceutical wastewater treatment plant of Xinhua Pharmaceutical Co., Ltd. (Zibo, China). Substrate utilization tests showed that OA1 utilizes naphthalene, phenol, benzoate, salicylate, and protocatechuate as the sole carbon and energy sources for growth. A degradation assay revealed that phthalate is an intermediate in naphthalene degradation and that the protocatechuate pathway plays an important role in naphthalene degradation. To determine the genetic basis and regulation of protocatechuate catabolism in OA1, a fosmid genomic library was constructed and a positive clone carrying the protocatechuate degradation gene cluster was isolated. Sequencing and a bioinformatics analysis identified the complete gene cluster, pcaJIGHBARC, responsible for protocatechuate degradation. Based on this gene cluster, the genes pcaGH (encoding the α and β subunits of protocatechuate 3,4-dioxygenase, 3,4-PCD) were coexpressed and the expressed products showed 3,4-PCD activity. This study illustrates a potential pathway of naphthalene degradation and identifies a protocatechuate pathway in Rhodococcus ruber OA1 for the first time, thus extending our understanding of polycyclic aromatic hydrocarbon degradation and the related aromatic compounds degraded in the process. Keywords Biodegradation Naphthalene Rhodococcus ruber Gene cluster Protocatechuate 3,4-dioxygenase