Identification of miRNAs and their targets by high-throughput sequencing and degradome analysis in cytoplasmic male-sterile line NJCMS1A and its maintainer NJCMS1B of soybean

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• 作者：Xianlong Ding ; Jiajia Li ; Hao Zhang ; Tingting He ; Shaohuai Han ; Yanwei Li…
• 关键词：Soybean (Glycine max (L.) Merr.) ; Cytoplasmic male sterility ; MicroRNA ; High ; throughput sequencing ; Degradome analysis
• 刊名：BMC Genomics
• 出版年：2016
• 出版时间：December 2016
• 年：2016
• 卷：17
• 期：1
• 全文大小：1,501 KB
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• 作者单位：Xianlong Ding (1)
  Jiajia Li (1)
  Hao Zhang (1)
  Tingting He (1)
  Shaohuai Han (1)
  Yanwei Li (1)
  Shouping Yang (1)
  Junyi Gai (1)
  
  1. Soybean Research Institute, National Center for Soybean Improvement, MOA Key Laboratory of Biology and Genetic Improvement of Soybean (General), State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing, Jiangsu, 210095, China
  
• 刊物主题：Life Sciences, general; Microarrays; Proteomics; Animal Genetics and Genomics; Microbial Genetics and Genomics; Plant Genetics & Genomics;
• 出版者：BioMed Central
• ISSN：1471-2164

文摘

Background Cytoplasmic male sterility (CMS) provides crucial breeding materials that facilitate hybrid seed production in various crops, and thus plays an important role in the study of hybrid vigor (heterosis), in plants. However, the CMS regulatory network in soybean remains unclear. MicroRNAs (miRNAs) play crucial roles in flower and pollen development by targeting genes that regulate their expression in plants. To identify the miRNAs and their targets that exist in the soybean CMS line NJCMS1A and its maintainer NJCMS1B, high-throughput sequencing and degradome analysis were conducted in this study.