Semiquantitation of diphenidine in tissue sections obtained from a human cadaver in a poisoning case by direct MALDI-QTOF mass spectrometry

详细信息    查看全文

• 作者：Kayoko Minakata ; Itaru Yamagishi ; Hideki Nozawa ; Koutaro Hasegawa…
• 关键词：Diphenidine ; Frozen section ; MALDI ; QTOF mass spectrometry ; Adipose tissue ; Brain ; Standard addition method
• 刊名：Forensic Toxicology
• 出版年：2016
• 出版时间：January 2016
• 年：2016
• 卷：34
• 期：1
• 页码：151-157
• 全文大小：851 KB
• 参考文献：1.Hasegawa K, Wurita A, Minakata K, Gonmori K, Nozawa H, Yamagishi I, Watanabe K, Suzuki O (2015) Postmortem distribution of AB-CHMINACA, 5-fluoro-AMB and diphenidine in body fluids and solid tissues in a fatal poisoning case: usefulness of the adipose tissue for detection of drugs in the unchanged forms. Forensic Toxicol 33:45–53CrossRef
  2.Minakata K, Yamagishi I, Nozawa H, Hasegawa K, Wurita A, Gonmori K, Suzuki M, Watanabe K, Suzuki O (2015) Diphenidine and its metabolites in blood and urine analyzed by MALDI-Q-TOF mass spectrometry. Forensic Toxicol 33:402–408CrossRef
  3.Reyzer ML, Hsieh Y, Ng K, Korfmacher WA, Caprioli RM (2003) Direct analysis of drug candidates in tissues by matrix-assisted laser desorption/ionization mass spectrometry. J Mass Spectrom 38:1081–1092PubMed CrossRef
  4.Wang HYJ, Jackson SN, McEuen J, Woods AS (2005) Localization and analyses of small drug melecules in rat brain tissue sections. Anal Chem 77:6682–6686PubMed CrossRef
  5.Hsieh Y, Casale R, Fukuda E, Chen J, Knemeyer I, Wingate J, Morrison R, Korfmacher W (2006) Matrix-assisted laser desorption/ionization imaging mass spectrometry for direct measurement of clozapine in rat brain tissue. Rapid Commun Mass Spectrom 20:965–972PubMed CrossRef
  6.Kuwayama K, Tsujikawa K, Miyaguchi H, Kanamori T, Iwata YT, Inoue H (2011) Distribution measurement of amphetamine-type stimulants in organs using micropulverized extraction and liquid chromatography/tandem mass spectrometry to complement drug distribution using mass spectrometry imaging. Rapid Commun Mass Spectrom 25:2397–2406PubMed CrossRef
  7.Kuwayama K, Tsujikawa K, Miyaguchi H, Kanamori T, Iwata YT, Inoue H (2012) Distribution measurements of 3,4-methylenedioxymethamphetamine and its metabolites in organs by matrix-assisted laser desorption/ionization imaging mass spectrometry using an automatic matrix spraying system with an air brush and a turntable. Anal Bioanal Chem 404:1823–1830PubMed CrossRef
  8.Koeniger SL, Talaty N, Luo Y, Ready D, Voorbach M, Seifert T, Cepa S, Fagerland JA, Bouska J, Buck W, Johnson RW, Spanton S (2011) A quantitation method for mass spectrometry imaging. Rapid Commun Mass Spectrom 25:503–510PubMed CrossRef
  9.Landgraf RR, Garrett TJ, Prieto Conaway MC, Calcutt NA, Stacpoole PW, Yost RA (2011) Considerations for quantification of lipids in nerve tissue using MALDI mass spectrometric imaging. Rapid Commun Mass Spectrom 25:3178–3184PubMed PubMedCentral CrossRef
  10.Wurita A, Hasegawa K, Minakata K, Gonmori K, Nozawa H, Yamagishi I, Suzuki O, Watanabe K (2014) Postmortem distribution of α-pyrrolidinobutiophenone in body fluids and solid tissues of a human cadaver. Leg Med 16:241–246CrossRef
  
• 作者单位：Kayoko Minakata (1)
  Itaru Yamagishi (1)
  Hideki Nozawa (1)
  Koutaro Hasegawa (1)
  Kunio Gonmori (1)
  Masako Suzuki (2)
  Amin Wurita (1)
  Osamu Suzuki (1)
  Kanako Watanabe (1)
  
  1. Department of Legal Medicine, Hamamatsu University School of Medicine, 1-20-1 Handayama, Higashi-ku, Hamamatsu, 431-3192, Japan
  2. Research Equipment Center, Hamamatsu University School of Medicine, 1-20-1 Handayama, Higashi-ku, Hamamatsu, 431-3192, Japan
  
• 刊物主题：Forensic Medicine; Pharmacology/Toxicology; Medicinal Chemistry; Medicine/Public Health, general; Medical Law;
• 出版者：Springer Japan
• ISSN：1860-8973

文摘

Diphenidine [1-(1,2-diphenyl-ethyl)piperidine] in the tissue sections of the adipose, brain, heart, kidney, and liver obtained from a human cadaver in a poisoning case was directly analyzed as the first trial by matrix-assisted laser desorption ionization (MALDI)-quadrupole time-of-flight mass spectrometry (QTOF-MS). The frozen tissue block of approximately 5 × 5 × 5 mm3 was sectioned to a thickness of 10 or 20 μm at −20 or −35 °C. For quantitation, the reference standard solutions of diphenidine were placed at 0, 0.1, 1, and 10 ng/well on the wells of a MALDI sample plate firstly, and about 20 tissue sections (ca. 5 × 5 mm2 each) were placed on the sample plate secondly, followed by detection by MALDI-MS/MS after desiccation of the tissue sections and spraying α-cyano-4-hydroxycinnamic acid as the matrix. The quantitation was performed by the standard addition method without the use of blank human tissues. The correlation coefficients between the concentrations of diphenidine and the relative signal intensities were from 0.937 to 0.994 for five kinds of tissue. The suppression of signals by tissue was increased in the order of adipose tissue < brain < heart, kidney, and liver; hence, the adipose tissue was most suitable tissue among them for the detection of diphenidine. In the present method, the concentration linearity and repeatability were not satisfactory for quantitative analysis, but allowable for semiquantitation. Therefore, the present method can be used for a semiquantitative analysis of diphenidine in tissue sections because of the simplicity of the method. Keywords Diphenidine Frozen section MALDI-QTOF mass spectrometry Adipose tissue Brain Standard addition method