Human islet cells are killed by BID-independent mechanisms in response to FAS ligand

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• 作者：Mugdha V. Joglekar ; Prerak M. Trivedi ; Thomas W. Kay ; Wayne J. Hawthorne…
• 关键词：Human islets ; BID ; FAS ligand ; Type 1 diabetes ; Progenitor cells ; siRNA
• 刊名：Apoptosis
• 出版年：2016
• 出版时间：April 2016
• 年：2016
• 卷：21
• 期：4
• 页码：379-389
• 全文大小：6,217 KB
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• 作者单位：Mugdha V. Joglekar (1)
  Prerak M. Trivedi (2) (3)
  Thomas W. Kay (2) (3)
  Wayne J. Hawthorne (4)
  Philip J. O’Connell (4)
  Alicia J. Jenkins (1) (3)
  Anandwardhan A. Hardikar (1)
  Helen E. Thomas (2) (3)
  
  1. Diabetes and Islet Biology Group, NHMRC-Clinical Trials Centre, University of Sydney, Camperdown, Australia
  2. St. Vincent’s Institute of Medical Research, 41 Victoria Parade, Fitzroy, Melbourne, VIC, 3065, Australia
  3. Department of Medicine, St. Vincent’s Hospital, The University of Melbourne, Melbourne, Australia
  4. The Centre for Transplant and Renal Research, Westmead Millennium Research Institute, University of Sydney, Westmead, Australia
  
• 刊物类别：Medicine
• 刊物主题：Medicine & Public Health
  Oncology
  Cancer Research
  Cell Biology
  Biochemistry
  Virology
  
• 出版者：Springer Netherlands
• ISSN：1573-675X

文摘

Cell death via FAS/CD95 can occur either by activation of caspases alone (extrinsic) or by activation of mitochondrial death signalling (intrinsic) depending on the cell type. The BH3-only protein BID is activated in the BCL-2-regulated or mitochondrial apoptosis pathway and acts as a switch between the extrinsic and intrinsic cell death pathways. We have previously demonstrated that islets from BID-deficient mice are protected from FAS ligand-mediated apoptosis in vitro. However, it is not yet known if BID plays a similar role in human beta cell death. We therefore aimed to test the role of BID in human islet cell apoptosis immediately after isolation from human cadaver donors, as well as after de-differentiation in vitro. Freshly isolated human islets or 10–12 day cultured human islet cells exhibited BID transcript knockdown after BID siRNA transfection, however they were not protected from FAS ligand-mediated cell death in vitro as determined by DNA fragmentation analysis using flow cytometry. On the other hand, the same cells transfected with siRNA for FAS-associated via death domain (FADD), a molecule in the extrinsic cell death pathway upstream of BID, showed significant reduction in cell death. De-differentiated islets (human islet-derived progenitor cells) also demonstrated similar results with no difference in cell death after BID knockdown as compared to scramble siRNA transfections. Our results indicate that BID-independent pathways are responsible for FAS-dependent human islet cell death. These results are different from those observed in mouse islets and therefore demonstrate potentially alternate pathways of FAS ligand-induced cell death in human and mouse islet cells.